A widespread variety of toxicants modify miRNAs profiles in target tissues. Five rat treatment groups were selected for this study, each consisting of five animals and an additional one left untreated used as a control. Administration of the carcinogen 4-Nitroquinoline (4-NQO) lasted for 5 months with four weeks intervals separating the successive groups. When the carcinogen intake period ended, the animals were euthanized and renal tissue was collected for both histopathological and molecular investigations. The results showed no significant difference (p=0.65) between the animal groups that showed kidney tissue toxicity and those that did not. Conversely, a statistically significant difference emerged if the mean ΔCt of the gene of interest (miRNA-21) in the treated group (6.95±1.23) was compared to that of the control group (4.42±0.34) (p=0.000). A significant difference was also observed when the comparison involved miRNA-122 and miRNA-221 whose ΔCt values for the treated and control groups were 2.28±0.2, 1.19±0.51 (p=0.04);0.39±0.08,1.52±0.06 (p=0.02), respectively. In conclusion: The findings of the present work revealed variable degrees of renal tissue injury despite non-significant; but it emphasizes the significant alterations of the miRNA tissue levels, the latter can indicate the adoption of specific miRNAs as vital markers denoting the prediction and/or the diagnosis of tissue injury after exposure to certain chemicals.
NiTi (also called Nitinol) transforms from cubic (austenite) to monoclinic (martensite), and vice versa, owing to the shape memory effect and superelasticity. Nitinol has a large number of biomedical applications because of its low elastic modulus close to that of natural bone material and good resistance to corrosion and fatigue, in addition to the transformation temperatures of nitinol that are close to body temperature. It has many other important applications, such as in the aircraft industry. In all these important applications, especially medical applications, Nitinol stability is an important factor for safety. Our goal is to study the stability of NiTi by calculating the phonon dispersion relation to obtain an accurate u
... Show MoreNuclear medicine is important for both diagnosis and treatment. The most common treatment for diseases is radiation therapy used against cancer. The radiation intensity of the treatment is often less than its ability to cause damage, so radiation must be carefully controlled. The interactions of alpha particle with matter were studied and the stopping powers of alpha particle with ovary tissue were calculated using Beth-Bloch equation, Zeigler’s formula and SRIM Software also the range and Liner Energy Transfer (LET) and ovary thickness as well as dose and dose equivalent for this particle were calculated by using Matlab language for (0.01-200) MeV alpha energy.
As modern radiotherapy technology advances, radiation dose and dose distribution have improved significantly. As part of a natural evolution, there has recently been renewed interest in therapy, particularly in the use of heavy charged particles, because these types of radiation serve theoretical advantages in all biological and physical aspects. The interactions of alpha particle with matter were studied and the stopping powers of alpha particle with Breast Tissue were calculated by using Beth-Bloch equation, Zeigler's formula and SRIM software, also the Range and Liner Energy Transfer (LET) and Breast Thickness As well as Dose and Dose equivalent for this particle were calculated by using Mat lab language for (0.01-200) MeV alpha ene
... Show MoreJoining tissue is a growing problem in surgery with the advancement of the technology and more precise and difficult surgeries are done. Tissue welding using laser is a promising technique that might help in more advancement of the surgical practice. Objectives: To study the ability of laser in joining tissues and the optimum parameters for yielding good welding of tissues. Methods: An in-vitro study, done at the Institute of Laser, Baghdad University during the period from October 2008 to February 2009. Diode and Nd-YAG lasers were applied, using different sessions, on sheep small intestine with or without solder to obtain welding of a 2-mm length full thickness incision. Different powers and energies were used to get maximum effect. Re
... Show MoreThe present study was designed to show the potential role of Matricaria chamomilla flower extract against toxicity of Entamoeba histolytica . The study used 20 adult albino male rats that divide randomly to four groups (each group consist 5 rats); control group received ad libidium, group of rats administrated with 1X103 cyct/ml suspension for seven days, group of rats administrated with 1X103 cyct/ml suspension and treated with 50mg/kg extract for month, group of rats administrated with 1X103 cyct/ml suspension and treated with 100mg/kg extract for month. The results show high significant increased (P < 0.05) in levels of Alanine
... Show MoreBackground: The Epstein-Barr virus (EBV) relates to the torch virus family and is believed to have a substantial impact on mortality and perinatal events, as shown by epidemiological and viral studies. Moreover, there have been documented cases of EBV transmission occurring via the placenta. Nevertheless, the specific location of the EBV infection inside the placenta remains uncertain. Methods: The genomic sequences connected to the latent EBV gene and the levels of lytic EBV gene expression in placental chorionic villous cells are examined in this work. A total of 86 placentas from patients who had miscarriage and 54 placentas from individuals who had successful births were obtained for analysis. Results: The research employed QPCR to dete
... Show MoreTissue culture of Catharanthus roseus was established under many parameters to insure good results for detection of the alkaloids present in this plant . It was found that NItsch and Nitsch medium containing 8µM Benzyladeninpurine plus Naphalene acetic acid were the best and the callus of C.roseus left to grow in the dark and had much better influence for the production of Alkloids. The precursor phenylalanine showed a better result than other precursor( tryptophan ) . Abscisic acid has an inhibitory effect on the production of Alkaloid
To explore the potential for in vitro rapid regeneration of Spanish dagger (Yucca gloriosa 'Variegata'), different concentrations of 6-Benzyladenine (BA), 1-naphthaleneacetic acid (NAA) and combinations of both were evaluated for callus induction initiated on leaf and bud (terminal and axillary buds) explants using Murashige and Skoog (MS) medium. Callus response induction percentage, fresh weight, color and texture of the callus were assessed after 1.5 and 6.0 months in culture. The appropriate medium for callus initiation on leaf explants was MS medium supplemented with 6.0 mg/L NAA. A combination of 0.2 mg/L BA and 1.5 mg/L NAA also exhibited a remarkable callus induction on bud explants. Effect of thidiazuron (TDZ) addition to the cultu
... Show MoreThe objective of this research was to estimate the dose distribution delivered by radioactive gold nanoparticles (198 AuNPs or 199 AuNPs) to the tumor inside the human prostate as well as to normal tissues surrounding the tumor using the Monte-Carlo N-Particle code (MCNP-6.1. 1 code). Background Radioactive gold nanoparticles are emerging as promising agents for cancer therapy and are being investigated to treat prostate cancer in animals. In order to use them as a new therapeutic modality to treat human prostate cancer, accurate radiation dosimetry simulations are required to estimate the energy deposition in the tumor and surrounding tissue and to establish the course of therapy for the patient. Materials and methods A simple geometrical
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