Cutaneous Leishmaniasis (CL) is an endemic disease and one of the major health problems in Iraq. Leishmania tropica is known as the causative agent of Cutaneous Leishmaniasis in Baghdad.The classical serological methods of diagnosing leishmaniasis is a poor sensitivity especially for the sub genus and time consuming Here we have investigated two primer pairs, one specific for Leishmania as genus and the primer specific for the species of L. tropica to be detected by polymerase chain reaction (PCR).Samples were collected from (AL-karama Teaching Hospital) and whole genomic DNA was extracted from axenic promastigotes.The extracted DNA was amplified by PCRwith two KDNA primer pairs, for genus specific (13A/13B) and (Lmj4/Uni21) to identify the specific species of cutaneous leishmaniasis. Amplified PCR products then run on gel electrophoresis and two visible bands of the two primers were seen, 120 and 800 bp, respectively. Our results indicate that the PCR technique is sensitive and specific for the detection and differentiation of cutaneous leishmaniasis agents and can be recommended to applied in hospitals and research centers.
The current study was conducted on 100 females who were divided into two main groups; 60 with breast cancer and 40 healthy controls. Blood samples were collected from both premenopausal and postmenopausal breast cancer and healthy women. The samples were appropriately processed for the analysis of trace elements (zinc, copper, and lead) by using flame atomic absorption spectrophotometry (FAAS). The results showed a highly significant decrease (p< 0.01) in the mean serum level of zinc of in both pre- and postmenopausal breast cancer women (71.7 + 5.1 and 70.4 + 5.4 µg/dL, respectively) compared with healthy controls (89.7 + 10.2 and 97.5 + 13.2 µg/dL, respectively) . Also, a highly significant
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The aim of this research is to measure the effect of Adey- Shire model in the achievement and critical thinking of first intermediate female students in mathematics. The researcher adopted the experimental method with a post-test, the research of sample consists of (60) female students, divided into two groups with (30) students in the experimental group, that studied with Adey- Shire model, and (30) students in the control group who studied in the usual way. The two groups are equivalent in many variables. The researcher makes two tests of multiple choices, the first one is an achievement test consists (30) items and another test was for a critical thinking test with (25) items. The statistical analysis make to both tests is made with s
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In this research (100* 40* 4 cm) solar cell panel was used in Baghdad at autumn season (2010), to get best solar cell panel angles experimentally, and then a mirror (40*50 cm) is use to concentrate incident sunlight intensity on a panel. At first case we get (Tilt angle ?P =60°and Surface Azimuth angle ?P =36°E) is the best angles and other case, we add a mirror at angle = 120° at bottom of panel, then we get output power (27.48watt) is bigger than without using a mirror (25.16watt). We can benefit from these cases in variety applications.
Twenty four samples of various granites and marble used common as flooring materials were collected from many factories and market in Baghdad Province, Iraq. Natural radioactivity of 226Ra, 232Th and 40K in the samples was measured by using high purity germanium detectors with an efficiency of 40%. The Specific activity concentration in granite samples ranged from 9.20 to 79.64 Bq/kg, 10.50 to 229.40 Bq/kg and 185.30 to 1391.20 Bq/kg for 226Ra, 232Th and 40K, respectively. The estimated of radiological parameters: radium equivalent activity (Req), activity concentration Index (I) and hazard indices (internal (Hin) and External (H
... Show MoreBrain tissues segmentation is usually concerned with the delineation of three types of brain matters Grey Matter (GM), White Matter (WM) and Cerebrospinal Fluid (CSF). Because most brain structures are anatomically defined by boundaries of these tissue classes, accurate segmentation of brain tissues into one of these categories is an important step in quantitative morphological study of the brain. As well as the abnormalities regions like tumors are needed to be delineated. The extra-cortical voxels in MR brain images are often removed in order to facilitate accurate analysis of cortical structures. Brain extraction is necessary to avoid the misclassifications of surrounding tissues, skull and scalp as WM, GM or tumor when implementing s
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