Poliomyelitis is a viral disease caused by an enterovirus known as poliovirus and is well known for its role in causing paralysis in children, the virus is only infectious in humans and does pass into the central nervous system and cause various degrees of paralysis, poliovirus passes newcomer disabuse of suppliant to alms-man thumb the fecal-oral route infected persons still shed the virus in their stool allowing the virus to infect others. The main aim of this study was isolating and differentiation of poliovirus strains (Sabin virus) from the stool samples of children received polio vaccine _TOPV and suffering from acute flaccid paralysis.

In this study use the cell culture system as the gold-standard method for Culture and isolation of Sabin polioviruses newcomer disabuse of the clinical moderate samples of sharp-witted flaccid paralysis AFP cases.     

Total of two hundred and fifty (250) stool test was taken from vaccinated children with _TOPV who suffering from acute paralysis in Iraq.The samples were collected during the period of the study from (March to November in the  year  2017) Were transferred under cold situation the children were stratified according  to provinces .Stool test  taken within 48 hours were each patient  before the fourteenth days after the onset of  AFP .            

The study that 35 (14%) samples were positive for Sabin for  poliowere isolated from AFP cases. Showed CPE effect on L20B cells only all the samples were from patient vaccinated with the type of  OPV in  varying number  of orally  _TOPV dose.

The current study which was done in different Iraqi provinces shows that (Sabin for polio of type 3) were isolated from AFP cases had the highest prevalence affecting 18 cases (51. 42 %) out of 35 Sabin of polioviruses AFP. The present study  showed that the maximum frequency of  (Sabin for  polioviruses typo 3) virus isolate was reported  in Baghdad 7 ( 38.8%) and  both provinces Anbar  and Salahdeen shows 3  (16.6%) followed by Wasit  and Dyiala  2 case (11.11%),  then Duhok  1  case  (5.5%).

Ten 10 cases (28.57%) of the Sabin AFP polio  were mixing  isolated (Sabin of  polioviruses typo 3 with   type 1) these positive cases found in Anbar province in high prevalence 4  cases (40%) followed by Baghdad, Erbil 2 case (20 %)  for each then , Duhok and Tamim 1 case ( 10 %) .

Seven 7 (20 %) stool samples were positive for Sabin for  polio  type 1  serotype were isolated from AFP cases the large  number of the diagnosis was obtained from a patient  in the Baghdad  isolate 3 cases  (42.85 %) ) followed by Sulymania 2positive  cases (28.57 %) then  Qadysia and Basrah  1 case (14.28% .