According to the prevalence of multidrug resistance bacteria, especially Pseudomonas aeruginosa, in which the essential mechanism of drug resistance is the ability to possess an efflux pump by which extrusion of antimicrobial agents usually occurs, this study aims to detect the presence of mexB multidrug efflux gene in some local isolates of this bacteria that show resistance towards three antibiotics, out of five. Sensitivity test to antibiotics was performed on all isolates by using meropenem (10µg/disc), imipenem (10µg/disc), amikacin (30 μg/disc), ciprofloxacin (5µg/disc) and ceftazidime (30 µg/disc). Conventional PCR results showed the presence of mexB gene (244bp) in four isolates out of ten (40%). In addition,25, 50μg/ml of curcumin was used to detect its efficacy with the antibiotics that the bacteria showed resistance towards. Results showed the highest resistance for ciprofloxacin (80%), while all of them were sensitive to imipenem. In addition, the present results show that both concentrations of curcumin (25, 50μg/ml) were effective in increasing the zone of inhibition from zero to 10 mm for isolates towards amikacin. Same result was obtained towards ciprofloxacin, except for an increase of inhibition zone from zero to 7 mm to one isolate (38T) when treated with 50 μg/ml, and finally an increase in sensitivity to ceftazidime was found and inhibition zone was increased from 8 to 11 for the second isolate (42E), which revealed that curcumin potentiates antibiotics activity by inhibition of efflux pump mechanisms that can be related to the synergetic activity between antibiotics and curcumin.
Abstract
urgent surgical intervention and appendectomy until now represent standard treatment for uncomplicated cases of appendicitis. Nausea, vomiting and pain after surgery are the most frequent issues facing patients and affecting patient quality of life and responsible for many cases of readmission after surgery. Ketorolac and diclofenac represent the most commonly prescribed non-steroidal anti-inflammatory used in postoperative setting and they cause many side effects as gastrointestinal, kidney and cardiac adverse effect in addition to increased risk of bleeding. Paracetamol is currently among the most frequently prescribed medication worldwide and it can be used safely for all age groups. This study aimed
... Show MoreE-learning is a necessity imposed by the Corona pandemic, which has disrupted various educational institutions in the world, but some of these institutions have not been affected and education has continued with them, due to their flexible educational system that was able to employ technology in the continuity of the educational process in the so-called e-learning, because It has characteristics that make it the most suitable alternative to avoid the consequences of the Corona pandemic and its damage to the educational process, as e-learning is one of the modern methods that contribute to enhancing the effectiveness of the learner, and enabling him to assume greater responsibility compared to traditional education, so the learner becomes
... Show MoreBackground: Gasoline constituents and its derivatives had many hazardous effects on the general health of humans. Thus, gasoline stations workers may be affected by different types of related diseases.This study was conducted to assess selected salivary elements and their relation with dental caries, oral hygiene status and periodontal diseases among gasoline stations workers in comparison with individuals have no regular exposure to gasoline. Materials and methods: The study group consists of thirty male subjects with an age range (33-39) years who worked in different gasoline stations in different areas of Baghdad city and thirty persons that matching in age and gender and not exposed to gasoline were selected as a control group. Dental c
... Show MoreSufism has aesthetic signs linked to the human spirit and adopts a set of spiritual, motor and tonal practices, let alone the philosophical aspects that have been tackled in the epics, novels and theatrical texts, the theatrical show can recall the Sufi image and its aesthetics within the operating and image system of the theatrical show.. The research problem resides in how to employ the Sufi image in the theatrical show. The research consists introduction of the research subject, the problem, the importance, and objectives represented by uncovering the aesthetic and intellectual representations of the Sufi image in the theatrical show. the theoretical foundation within two sections: the first highlighted the concept of (Sufism a
... Show MoreMishrif Formation regards one of the most important reservoirs in Iraq. Well logging represents one of the most important tool in the formation evaluation. According to the Petrophysical properties that have been gotten from well logging, Mishrif Formation in terms of reservoirs units, consist of several reservoirs units. Major reservoirs units divided into three reservoir units,MA,MB&MC. Each of these major units divided into minor reservoirs units (MB11,MB12,MC2&MC3).MB major reservoir units represent the best reservoir unit. These reservoirs units separated by cap rocks(mainly tight limestone)(CR1,CR2,CR3,CR4,CR5,CR6,and CR7).CPI were demonstrated for all wells.Hydrocarbon saturation vs.
... Show Morethe first part of the research involves investigate the aspect of the radiation superposed on the one bright soliton pulse propagated on ideal single mode
The magnetic field effect on the current-voltage characteristic curves of glow discharge in argon at low pressures has been experimentally investigated. The electrical discharge was ignited in a stainless steel tapered chamber of a nominal volume of 0.5m3 immersed inside a water-cooled coil capable of delivering a magnetic field of strength B of up to 0.42T. Three water-cooled electrodes were inserted into the chamber up to a point where their tips were 20cm away from the surface of the central column of the chamber. An enhancement of the electric field configuration within the region of the electrode assembly was performed by threading one of the electrodes with stainless circular discs(80mm and 140mm in diameter) in various forms(attac
... Show MoreA simple and highly sensitive cloud point extraction process was suggested for preconcentration of micrograms amount of isoxsuprine hydrochloride (ISX) in pure and pharmaceutical samples. After diazotization coupling of ISX with diazotized sulfadimidine in alkaline medium, the azo-dye product quantitatively extracted into the Triton X-114 rich phase, dissolved in ethanol and determined spectrophotometrically at 490 nm. The suggested reaction was studied with and without extraction and simple comparison between the batch and CPE methods was achieved. Analytical variables including concentrations of reagent, Triton X-114 and base, incubated temperature, and time were carefully studied. Under the selected opti
... Show MoreNitroso-R-salt is proposed as a sensitive spectrophotometric reagent for the determination of paracetamol in aqueous solution. The method is based on the reaction of paracetamol with iron(III) and subsequent reaction with nitroso-R-salt to yield a green colored complex with maximum absorption at 720 nm. Optimization of the experimental conditions was described. The calibration graph was linear in the concentration range of 0.1 – 2.0 ?g mL-1 paracetamol with a molar absorptivity of 6.9 × 104 L mol-1 cm-1. The method was successfully applied to the determination of paracetamol in pharmaceutical preparations without any interference from common excipients. The method has been statistically evaluated with British Pharmacopoeia method a
... Show MoreContemporary management is interested in the process of performance assessment because of its significance in the field of planning and controlling the multiactiveties to attain its goals and uncovering digression of virtual performance after comparing it with the plan or equitable performance.
Digression is analyzed to enable management control centers of authority.
Assessment process significance is closely related by setting definite categories to evaluate the economic activity to know the ability to achieves aims.
This study concentrated on the most important categories that used to evaluate company understudy with ather categories suggested to complete assessment process.
This study is in
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