Due to their high nutritional value, richness in carbohydrates and good source of protein, legumes are considered essential for human diet. Legumes flours were used as fermentable material for lactic acid production. Hence, previously isolated lactobacilli strains were used in this study. The strains showed strong microbial growth and their survival in glucose-containing MRS medium and were described using a modified Gompertz equation. Lactobacilli exhibited the shortest latency phase in MRS-glucose medium. While the highest lactic acid produced was 15.40g/l by Lpb. pentosus U1 isolate after 48hours. Furthermore, these strains were evaluated in flours samples for acidification capacity. The results showed a decrease in pH values after 12hours of fermentation, indicating the fastest acidification with maximum lactic acid generation of 31.66, 25.33, 31.00 and 31.66g/l for black gram flour by Lpb. plantarum M1, Lpb. pentosus U1, Lpb. plantarum VIP1 and Lev. brevis TIP1 isolates. Black gram flour was considered the best among the used substrates for enhancing lactic acid production. Overall, this study can be termed as a preliminary step for fermentation using the desired organism in preparing legume-based food at the industrial level and benefiting consumers.
The purpose of this preliminary study is to verify the possibility of using Iraqi Zahdi date palm biomass as a resource for biogas production, methane in particular using thermophilic anaerobic digestion with waste water treatment activated sludge. Moreover, is to investigate the influence of extra nutrients addition to the digestion mixture. Biogas was captured in sealed jars with remote sensing modules connected to computer with integrated program to record the gas pressure continuously. A total gas pressure with 67% Methane was produced from date pulp waste fermentation with a yield of 0.57 Lit for each gram volatile solid of substrate. Addition of 1% yeast extract solution as nutrient increased Methane yield in liters by 5.9%. This i
... Show MoreBackground: P. aeruginosa remains a important cause of life threatening bloodstream infection in immunocompromised patients, particularly those with hematologic malignancies complicated by neutropeni.
This study investigates the digestion of cow dung (CD) for biogas production at laboratory scales. The study was carried out through anaerobic fermentation using cow dung as substrate. The digester was operated at ambient temperatures of 39.5 °C for a period of 10 days. The effect of iron powder in controlling the production of hydrogen sulfide (H2S) has been tested. The optimum concentration of iron powder was 4g/L with the highest biogas production. A Q – swatch Nd:YAG laser has been used to mix and homogenize the components of one of the six digesters and accelerate digestion. At the end of digestion, all digestions effluent was subjected to 5 laser pulses with 250mJ/pules to dispose waste biomass.
In this study, detection of uricase production from Pseudomonas aeruginosa
isolates was done by applying colorimetric method, Uricase was purified from the
most potent isolate by precipitation using ammonium sulphate (80% saturation) then
purification was achieved using DEAE –Cellulose ion exchange and Sepharose 6B
gel filtration chromatography column, 16.4% of total enzyme was recovered with
specific activity 2337.5U/mg and 22.21folds of purification. Characterization of
uricase involved detection of optimal conditions for uricase activity, the maximal
activity was obtained at temperature 45ºC,while uricase appeared to be stable at
40ºC. Uricase showed optimal activity at pH 9 while pH stability was in the
The present study aimed to try to find natural substances stimulate the production of bacteriocin, as well as "for detection of bacteriocin producing isolates. Two hundred and eighty ( 280) bacterial isolates, gram negative only, were collected from 760 different pathogenic samples, consist: (Urinary tract infection, septicemia, Vaginal inflammation and diarrhea). The isolated bacteria are: Escherichia coli, Klebsiella pneumonia Pseudomonas aeruginosa,, Salmonella typhi, Enterobacter cloacae, Acinetobacter baumannii, Serratia liquefaciens, Citrobacter freundii, Proteus mirabilis and Serrattia odorifera. Cup assay method was used to detect bacteriocin production. Loc
... Show MoreSuccinic acid is an essential base ingredient for manufacturing various industrial chemicals. Succinic acid has been acknowledged as one of the most significant bio based building block chemicals. Rapid demand for succinic acid has been noticed in the last 10 years. The production methods and mechanisms developed. Hence, these techniques and operations need to be revised. Recently, an omnibus rule for developing succinic acid is to find renewable carbohydrate Feedstocks. The sustainability of the resource is crucial to disintegrate the massive use of petroleum based-production. Accordingly, systematically reviewing the latest findings of bacterial production and related fermentation methods is critical. Therefore, this paper aims to stud
... Show MoreThe genus Bacillus bacterium isolated from soil and tests their ability to produce glutamic acid, the bacteria are diagnosed by traditional methods and VITEK-2 system. And we studied the optimal conditions for production and try to improve the productivity of the isolation by manipulating the components of the production medium and of the circumstances environmental of production medium. There is 43 isolates of the 70 bacterial isolates belonging to the genus Bacillus depending on phenotypic characteristics of the colonies and microscopic characteristics and found that 13 isolated revert to species B.subtilis depending on physiological and biochemical characteristics. As well as on microscopic and biochemical tests, 13 isolates were subj
... Show MoreMicroorganisms have an active role in biotechnology for example yeasts, especially in some genus like Saccharomyces, Pichia, and Candida. C.tropicalis one of the most important species of Candida and despite it is one of the causative agents of candidiasis but it has a major role in the production of many chemical compounds. C.tropicalis in the previous study was isolated from sheep dung and morphologically and molecularly classified the result of sequencing was elucidate 100% similarity between the studied isolate and other isolates inserted in DNA Data Bank of Japan DDBJ, physiologically this isolate tolerated 6% ethanol concentration in broth media with the ability to the pro
... Show MoreIn this study some generic commercial products of Atorvastatin tablets were evaluated by dissolution test in acid medium by comparing with that of parent drug Lipitor of Pfizer Company. Some of solubilizing agents were studied in formulation of Atorvastatin tablet including; surface active agent and PEG 6000 .The most effective factor was the use of PEG6000 in formulation of Atorvastatin tablet which improved the dissolution and the results of dissolution profile of formulated tablet in this work was bioequivalent to that of Lipitor .The quantitative analysis of this work was performed by using reversed phase liquid chromatography and a proper mixture of  
... Show MoreThe research aims to derive the efficient industrial plans for Al – shaheed public company under risk by using Target MOTAD as a linear alternative model for the quadratic programming models.
The results showed that there had been a sort of (trade- off) between risk and the expected gross margins. And if the studied company strives to get high gross margin, it should tolerate risk and vice versa. So the management of Al- Shaheed Company to be invited to apply the suitable procedures in the production process, in order to get efficient plans that improves it's performance .