This study aimed to evaluate the relationship between the microbiota number of some common bacterial species in children's gut with Entamoeba histolytica infection. To achieve that,49 samples of stool were collected, 29 from children with acute amebiasis and 20 from healthy children, children's ages ranging from 1 month to 12 years from both genders. Counting three types of bacteria Lactobacillus spp., Bifidobacterium spp. and Escherichia coli, using the standard curve obtained from Real-Time PCR was accomplished. The standard curve was made from decimal serial dilution of samples containing an unknown number of bacteria. The results showed that E. histolytica infection creates considerable changes in the intestinal microbiota numbers. Lactobacillus spp., Escherichia coli and Bifidobacterium spp. numbers were affected. The mean values of Lactobacillus spp. and Escherichia coli in the patient's group (children with amebiasis) were increased compared to the control group (healthy children). The mean value in the patient groups were (1947.4) and (430657.9), while in the control groups were (1400.16) and (193927.7) for Lactobacillus spp. and Escherichia coli, respectively. Whereas the mean value of Bifidobacterium spp. showed a significant decrease in the patient group (103.875) compared with the control group (166.75). In conclusion, this study show alteration in predominant gut bacteria in E. histolytica infected children.
The aim of present study is to investigate the antagonistic activity of Spirogyra micropunctata against multidrug resistant human pathogens. The test organisms include 3 Gram negative bacteria such as Echerichia coli, Klebsiella pneumoniae, Salmonella typhi and 2 Gram positive bacteria such as Staphylococcus aureus and Staphylococcus epidermidis. The algal cell mass was extracted in 90% Methanol and 90% Ethanol and further concentrations of 0.5, 1, 3, 5, 10, 20 mg/ml were made for each extract. Antagonistic effect was tested by using agar well-diffusion method. Methanolic crude extract showed strong antibacterial activity against all tested bacteria, while ethanolic crude extract showed moderate activity. These findings suggest the possi
... Show MoreLactiplantibacillus plantarum, one of lactic acid bacteria (LAB), is found in various foods, including dairy products, meat, and vegetables, and most of these bacteria offer beneficial effects to humans and animals as potential probiotics with broad-spectrum antimicrobial activities. The aim of this study was evaluating the antibacterial efficacy of L. plantarum against some foodborne bacteria isolated from dairy products. This research involved 34 dairy products, including local and imported milk, cheese, and yogurt sold locally in Baghdad province, Iraq, during May 2022. For the isolation of L. plantarum, a special medium called MRS (de Man Rogosa and Sharpe) was applied. Colonie
... Show MoreAdhrt all fungal biological control ability Tdhadah less than 2 repel Alaftran Almamradan showed leaky mushroom Biological control is thermally laboratories and different concentrations of 5, 10 and 20% inhibition in the growth of fungus colonies amounted to 3.8 cm and 3.1 and 2.4 respectively in comparison with control 9 cm
The Leishmania donovani parasite causes visceral leishmaniasis (VL), an acute and fatal form of leishmaniasis. Because traditional therapy alternatives, such as glucantime and other pentavalent medicines, are toxic and have side effects, new treatments with fewer negative effects are needed. Only a handful of drugs are clinically beneficial to treatments of the disease, but considerable limitations threaten their very usage. Novel, safe, and efficient drugs, including those against antimalaria and leishmaniasis co-infections, are so essential. Artemether (ATM) is an Artemisinin derivative that has been demonstrated to be useful in the treatment of malaria and, more recently, leishmaniasis. The current research was carried out to evaluate th
... Show MoreBackground: Cerebral palsy is non-progressive disorder of posture or movement due to a lesion of the developing brain. It is the commonest physical disability in childhood. Objective: To study the clinical, neurological abnormalities, prevalence of convulsion (epilepsy) & to asses the value of CT scans of brain in patients with cerebral palsy.
Background: Cytokines have long been implicated in the pathogenesis of autoimmune diabetes in a number of studies, and playing a role in the initiation of β-cell damaging process. The objective of this study is to gain more understanding about the role of cytokines in initiation of T1DM, through assessment of IFN-γ, IL-10 and IL-6 in diabetic patients.
Patients and methods: A total of 60 patients who were newly diagnosed as having T1DM (diagnosed less than five months) were included in the present study. Fifty apparently healthy control subjects were underwent the measurement of serum IFN-γ, IL-10 and IL-6 by ELISA.
Results: Higher serum levels of IFN-γ, IL-10, and Il-6 were observed in the investigated
A field experiment was conducted at botanical garden of Department of Biology, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, during the growth winter season of 2016-2017 to study the effect of different concentrations (0, 10, 20) mg.L-1 of abscisic acid and (0, 50, 100, 150) mg.L-1 of vitamin C and their interaction on some plant hormones of pea plant (Pisum sativum L.). The results showed that ABA 20 mg.L-1 decreased IAA about 27.44%, GA3 about 19.73% and Kinetin 15.37% while vitamin C with 150 mg.L-1 increased IAA 27.43%, GA3 45.31% and Kinetin 58.53%, but ABA increased about 23.01% for ABA and 34.93% for vitamin C compared with
... Show Moreكانت وسائل الاعلام- التقليدية والحديثة – وستبقى وسيلة مهمة لتدفق المعلومات والتعبير عن المشاعر ووصف الاحداث وتشكيل الرأي العام المحلي والدولي عن مختلف القضايا التي تهم الافراد والمجتمعات وفي مقدمتها قضايا العنف بمستوياته كافة ،والتطرف الديني والارهاب بكل مسمياته وستحاول هذه الدراسة ان تقدم اجابات عن اسئلة محددة تمثل الاطار النظري لدراسات مسحية ترصد النظريات التي ترسم الاطر النظرية الت
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