Four molecular imprinted polymer (MIP) membranes for Mebeverine.HCl (MBV.HCl) were prepared based on PVC matrix. The imprinted polymers were prepared by polymerization of 2-acrylamido-2-methyl-1-propane sulphonic acid (AMPS) as monomer, pentaerythritoltriacrylate (PETRA) as a cross linker ,benzoyl peroxide (BPO) as an initiator and mebeverine as a template. Four different types of plasticizers of different viscosities were used and the electrodes were fully characterized in terms of plasticizer type, response time, lifetime, pH and detection limit.
The MBV-MIP electrodes exhibited Nernstian response in concentration range from 1.0×10-6 to1.0×10-1 M with slopes of 13.98, 19.60, -20.43 and 19.01 mV/ decade. The detection limit and quantification are found to be 4×10-6, 3× 10-6, 2× 10-5and 1.2× 10-6 M ,respectively. Interference study for inorganic cations and amino acids was also studied. The best electrode based tris (2-ethyl hexyl) phosphate plasticizer was applied successfully for potentiometric determination of mebeverine in commercial tablets. Excellent average recovery obtained by potentiometric methods was about 102.26% which is in good agreements with British Pharmacopoeia.
To determine the relationship between Helicobacter pylori infection and skin disorders, sixty six patients who suffering from skin diseases include chronic urticarial (CU) and atopic dermatitis (AD) who attended at Dermatological Clinic/ Al-Numan Teaching Hospital from the beginning of October 2015 to the end of January 2016 with age (6-62) have been investigated and compared to twenty two samples of apparently healthy individuals were studied as control group. All the studied groups were subjected to measurement of antiHelicobacter pylori IgG antibodies by enzyme linked immuno sorbent assay (ELISA) and detection of 16S rRNA and CagA genes by using singleplex and multiplex PCR methods. The results of current study revealed that there was a
... Show MoreA total of sixty raw milk samples were collected from (street vendors and shops) from Baghdad city, Iraq. The samples were inoculated into peptone water and, then, subcultured onto MacConkey agar and Blood agar. Identification of isolates was confirmed by microscopic examination, cultural characteristic, biochemical tests, Vitek (VITEK®2 system), and Biolog GN substrate reactions followed by 16S rRNA and specific genes sequencing. Of 60 raw cow’s milk samples, Providencia spp. were identified only in 4 samples (6.67%) and P. rettgeri was the most common, 2/4 (50%), followed by P. stuartii and P. vermicola, 1/4 (25%). Antimicrobial susceptibility tests were conducted against ten antibiotics by the disc diffusion method. All Provid
... Show MoreIn this study, out of 50 isolates of some nosocomial infections from some Baghdad hospitals, only 13 (26%) were identified as Escherichia coli. Depending on selective media, morphological and biochemical tests the species was then confirmed by molecular methods. Later on antimicrobial resistance test was performed by the Kirby-Bauer method. The molecular characterization of blaTEM and blaCTX-M genes in different clinical isolates of E. coli was done through polymerase chain reaction (PCR) by utilizing special primers. These genes were positive to only 4 (30.7%) isolates. The sequence of nucleotides of positive genes was carried out for four isolates. The results showed that there was no vari
... Show MoreMultiple sclerosis (MS) is a neuro-inflammatory disorder in which the Epstein-Barr virus (EBV) is proposed to have a pathogenic role. Therefore, a case-control study was performed (93 patients with relapsing-remitting MS and 113 healthy controls (HC) to analyze the prevalence and viral load of EBV infection using real time-polymerase chain reaction. Prevalence of EBV infection was lower in patients compared to HC but the difference was not significant (12.9 vs. 21.2%; probability [p] = 0.187). EBV-positive MS cases were more common in females than in males (83.3 vs. 16.7%), while an opposite distribution was observed in HC (37.5 vs. 62.5%), and the difference was significant (p = 0.041). Blood group O frequency was higher in EBV-p
... Show MoreIn this study, the development of an indirect spectrophotometric method for the determination of folic acid in pure and pharmaceutical preparations is described. The method is based on the oxidation of pyrocatechol with iron (III) in an acidic medium, followed by the reaction with folic acid (FA) to produce a stable, water-soluble orange compound with maximum absorption at 350 nm versus the blank reagent. The complex of charge transfer was studied under optimal conditions; the titration graph was linear over the range of 0.5-25 μg/mL with a relative error of 1.2-2.8 and a relative standard deviation of 2.43-1.45 depending on the concentration level.
Lymphoma is a cancer arising from B or T lymphocytes that are central immune system components. It is one of the three most common cancers encountered in the canine; lymphoma affects middle-aged to older dogs and usually stems from lymphatic tissues, such as lymph nodes, lymphoid tissue, or spleen. Despite the advance in the management of canine lymphoma, a better understanding of the subtype and tumor aggressiveness is still crucial for improved clinical diagnosis to differentiate malignancy from hyperplastic conditions and to improve decision-making around treating and what treatment type to use. This study aimed to evaluate a potential novel biomarker related to iron metabolism,
... Show MoreEnterococci are usually encountered and predominate in oral infections, especially those associated with dental root canal infections of necrotic pulp and periodontitis. This study aimed to detect and identify Enterococcus faecium isolated from infected root canals, using polymerase chain reaction ( PCR). Thirty samples were collected from patients with necrotic pulp, infected root canals, and endodontic treatment failure, attending the Conservative Treatment Department, College of Dentistry, Mosul University, Dental Teaching Hospital. The samples were obtained by inserting sterile paper points into the root canals and transferred in brain heart infusion broth vials to be inoculated in a selective M-Enterococcus Agar Base . T
... Show MoreNanocrystalline ZnO/Zeolite type A composite was prepared by simple method of operation by . the precipitation of zinc oxide and loading on zeolite 5A in one step. Characterization was made by X-ray diffraction (XRD), X-ray fluorescence(XRF), N2 adsorption- desorption for BET surface area, and Atomic force microscopy (AFM). Results showed that zinc oxide was loaded on zeolite as noticed by the characteristic peaks and was of nano scale having an average diameter of 88.57nm. The percentage loading of ZnO on zeolite A was 28.37% and the surface area was 222m2/g. The activity of the prepared catalyst was examined in the desulfurization of double hydrogenated diesel fuel. The process was investigated in a
... Show MoreBackground: The temporal fascia has not been studied properly yet.