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Bacterial Biodegradation of Congo Red Dye Using Local Bacterial Isolates

     The current study aims to get local bacterial isolates isolated from wastewater samples, w the highest ability to decolourize one selected Azo dye (Congo red as a model), and then test its ability to decompose Congo red dye, to obtain the most efficient bacterial isolate

Four samples of wastewater collected from sewage transport pipes were used for the bacterial isolation. Forty-two bacterial isolates were obtained after inoculating these samples in the liquid MS medium, pH 7 with 1% glucose, and then on solid MS medium supplemented with50 ppm of Congo red dye. Results from primary tests showed that only eighteen bacterial isolates own varying abilities to decolourize Congo red dye and the isolates WR7, WR18 and WR30 give high clear zones (20 mm).

       The secondary screening was achieved to determine the most efficient isolates to degrade the Congo red dye. The results indicated that the isolates WR7, WR18, and WR30 appeared to have the highest ability to decolorize Congo red dye reaching 88.6%, 83.9%, and 92.8%, respectively.

The results from the optimum conditions experiment revealed that the isolates WR7, WR18 and WR30 appeared to have the highest ability to degrade dye reached 88.7%, 83.9%, and 92.9% respectively when 100 ppm of Congo red dye was used after 3 days of incubation as compared with other studied concentrations. While the second step in this experiment confirmed that the isolate WR30 has the highest percentage to degrade the Congo red dye during all incubation periods, which reached 14.6%, 42.7%, 92.9%, 92.4% and 91.8% after 1, 2, 3, 4, and 5 days of incubation when 100 ppm of Congo red dye was used respectively as compared with WR7 and WR18 isolates which showed lower values of degradation ​​for all studied periods.

The three isolates which have the highest capability to decolorize Congo red dye were identified according to morphological, physiological and biochemical tests. The results indicated that the isolates WR7, WR18 and WR30 were Bacillus subtilis, Pseudomonas aeruginosa, and Bacillus cereus respectively.

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Publication Date
Tue Dec 11 2018
Journal Name
Iraqi Journal Of Physics
Effect of annealing temperature on structural and optical properties of Cr2O3 thin films by PLD

In the present work, pulsed laser deposition (PLD) technique was applied to a pellet of Chromium Oxide (99.999% pure) with 2.5 cm diameter and 3 mm thickness at a pressure of 5 Tons using a Hydraulic piston. The films were deposited using Nd: YAG laser λ= (4664) nm at 600 mJ and 400 number of shot on a glass substrate, The thickness of the film was (107 nm). Structural and morphological analysis showed that the films started to crystallize at annealing temperature greater than 400 oC. Absorbance and transmittance spectra were recorded in the wavelength range (300-
4400) nm before and after annealing. The effects of annealing temperature on absorption coefficient, refractive index, extinction coefficient, real and imaginary parts of d

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Publication Date
Tue Jun 01 2021
Journal Name
Journal Of Planner And Development
Spatial analysis of rural services in the district of Fallujah, Zgharit village, as a model

          The study of services in villages is one of the imperative matters that must   be focused on, because it leads to increased attention, which reduces the differences between the countryside and the urban. The extent of its.

  It is well known that community services need to be reached by a person, unlike the anchor services that reach people, here the population distribution plays an important and prominent role in signing these services, so the dispersed distribution pattern and the gathering pattern appeared in the distribution, thus an effect on the time and distance that the person walked to obtain The services are community-based. Therefore

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Publication Date
Wed Nov 07 2018
Journal Name
Iraqi National Journal Of Nursing Specialties
Evaluation of the Hardness of Cold Cured Acrylic Resin Material by Processing at Different Temperature

Objective(s): This study aims to evaluate the hardness of two commercially available cold cured acrylic resin material
(Vertex and PAN) when polymerized at different temperature in comparison to those polymerized by conventional
methods in air at 23C ± 5C.
Methodology: Eighty specimens, forty from cold cured acrylic (Vertex Type) and forty from cold cured acrylic (PAN
type) were prepared, flasking and packing procedure were done according to manufacturer direction and divided
according to processing as follow: 20 specimens (10 from Vertex type and 10 from PAN type) were processed in air for
two hours at 23C ± 5C under press (bench curing) as a control, and 60 specimens (30 from Vertex type and 30 from
PAN type) wer

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Publication Date
Sun Jun 07 2015
Journal Name
Baghdad Science Journal
Separation and Determination of Some Organic Acids in Dry Calyces of Iraqi Hibiscus Sabdariffa Linn

A new reversed phase- high performance liquid chromatographic (RP-HPLC) method with Ultraviolet-Visible spectrophotometry has been optimized and validated for the simultaneous extraction and determination of organic acids present in Iraqi calyces of Hibiscus Sabdraffia Linn. The method is based on using ultrasonic bath for extracting organic acids. Limit of detection in µg/ml of Formic acid, Acetic acid, Oxalic acid, Citric acid, Succinic acid, Tartaric acid, and Malic acid 126.8498×10-6, 113.6005×10-6, 97.0513×10-6, 49.7925×10-6, 84.0753×10-6, 92.6551×10-6, and 106.1633×10-6 ,respectively. The concentration of organic acids found in dry spacemen of calyces of Iraqi Hibiscus Sabdraffia Linn. under study: Formic acid, Acetic acid,

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Publication Date
Mon Jan 31 2022
Journal Name
Iraqi Journal Of Science
Antibacterial activity of different part of Neem (Azadirchta indica) growing in Sharjah, United Arab Emirates

Aqueous and ethanolic extracts of different parts (seeds, leaves, bark) of neem plant (Azadirachta Indica) were screened for antibacterial activaties against five species of bacteria (Staphylococcus aurous, Staphylococcus epidermises, Acinetobacter baumanni, Psedommonas aeruginosa, and Escherishia coli). Different extracts 40-80 mg/L were tested using Ager-well diffusion method. Neem parts potent demonstrated for anti-bacterial activaties against all microorganisms tested. The results showed that neem seeds aqueous and ethanolic, extract have significant effects for all tested bacteria, the maximum inhibition zone by seeds cold aqueous and cold ethanolic extracts were 22 & 13 mm for E. coli and S. epidermidis respectively; while leav

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Publication Date
Wed Feb 08 2023
Journal Name
Iraqi Journal Of Science
Theoretical Design of Anticancer Agents of Some Crown Substituent's Through complexation with Cellular Potassium Ion

Theoretical study of ten crown ethers substituents were established to investigate some parameters that give clear view about their validity and applicability in the design of anticancer agents. Restricted hartree fock method (RHF/3-21G) were used to determine the energy difference between highest occupied molecular orbital and lowest unoccupied molecular orbital (HOMO-LUMO gap) , ionization energy, global hardness and total energy. Strong binding ability with potassium ion were obtained in some of these compounds depend on the type of substituents added to both nitrogen atoms out of the ring cavity. Such binding with potassium in abnormal and divided cancer cells result in inhibition of tumor cell growth by disrupting potassium ion home

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Publication Date
Sun Jul 01 2012
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The influence of smoking on male reproductive parameters in a group of Iraqi subfertile men

Background: Among different air pollutants, cigarette smoke contains toxic chemicals, mutagenic and carcinogenic compounds, which can adversely affect male fertility. In this study, semen parameters and reproductive hormonal concentrations of subfertile smokers were compared with subfertile non-smokers.
Objectives: evaluation of the effect of cigarette smoking on male fertility by evaluating several semen parameters as well as some reproductive hormones in a group of smoker and non smokersubfertile Iraqi subjects.
Patients and Methods: At the male infertility clinic of Al-yarmuk teaching hospital, Almustanseria medical college, Baghdad, Iraq from the 1st of October 2010 to the end of June 2011, 88 men (49 non-smokers, and 39 smoker

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Publication Date
Mon Mar 08 2021
Journal Name
Baghdad Science Journal
CALCULATION OF EXCESS MOLAR VOL-UMES OF SOME TERNARY LIQUID MIX-TURES AT 303.15 K

Excess molar volumes of five ternary mixtures of 2- methoxy ethanol(1) +butyl acetate(2)+benzene(3), +toluene(3), +chlorobenzene(3), +bromobenzene(3), and +nitrobenzene(3) have been measured at 303.15K. The excess molar volume exhibited positive deviation over the entire range of composition in the systems 2-methoxy ethanol(1)+ butyl acetate(2)+ benzene(3),+toluene(3) and sigmoid behavior in the case of the remaining systems. Flory's statistical theory have been extended to predict the excess molar volumes of the five ternary mixtures at 303.15 k over a wide range of composition . An excellent agreement has been found between the experimental and theoretical excess molar volumes , both in magnitude and sign .

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Publication Date
Tue Jan 01 2019
Journal Name
Aip Conference Proceedings
Physical properties of HgX Sb1-X Ba2Ca2Cu3O8+δ superconducting compound: Effect of fast neutrons irradiation

Applications of superconductor compounds were considered as modern and important topics, especially these which are exposures to one of the nuclear radiation kinds. So, we gone to investigate the influence of fast neutrons irradiation on electrical and structural characteristics of HgxSb1-xBa2Ca2Cu3O8+δ superconducting compound at (x = 0.7) in ratio. The superconducting specimens were synthesized using solid state technique. Specimens were exposure to the nuclear radiation using fast neutrons with doses (0, 9.06 x1010, 15.3 x 1010 and 18.17 x 1010) n/cm2 respectively. Electrical and X-ray diffraction properties of superconductor specimens before and after irradiation were investigated under standard conditions. Results of X-ray diffraction

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Publication Date
Sat Apr 15 2023
Journal Name
Iraqi Journal Of Science
3D seismic reflection study for subsurface structural picture of Dujaila Oil Field, South of Iraq

This research represents a 3D seismic structural study for 602.62 Km2 of Dujaila
Oil Field which is located 55 Km Northwest of Mysan province and 20 Km Southwest
of Ali-AlSharki region within unstable Mesopotamian basin.
Synthetic traces are prepared by using available data of two wells (Du-1, Du-2), in
order to define and pick the reflectors. Two reflectors are picked that represent the top
and bottom of Mishrif Formation, in addition to five units within this Formation are
picked, they named Units 1, 2, 3, 4, and 5.
Time maps for the top and bottom of Mishrif reflectors are drawn to get the
structural picture, these maps show general dip of layers toward NE, and thus, there
are two enclosure domes in the midd

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