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Biofilm Shows Independency form Hemolysin Genes Arsenal in Methicillin Resistant Staphylococcus Aureus

Normally, bacteria exposed to antibiotics at sub minimal inhibitory concentrations (MIC) inside the host. Therefore, the current study aimed to comprehend the association among hemolysins, biofilm, as well as gentamicin resistance in local MRSA isolates. Around 35 Staphylococcus aureus locally isolated from different clinical specimens were employed in this study. Methicillin resistance was detected via cefoxitin disk diffusion and mecA amplification methods. MIC of gentamicin was estimated by broth microdilution method. Hemolysin genes involving hla, hlb, hld, and hlg were determined using multiplex polymerase chain reaction (PCR) technique. Microtiter plate method was employed for biofilm assessment in the presence and absence of gentamicin at sub MIC. Moreover, atomic force microscopy technique was employed for confirming the effect of gentamicin on biofilm. The present findings revealed that methicillin resistant S. aureus (MRSA) constituted, nearly, 94.29% (33 isolates) of all S. aureus isolates. Around 12 (36.36%), four (12.12%), and 17 (51.51%) isolates were gentamicin-sensitive, intermediate, and resistant to gentamicin, respectively. hla and hld were located in 32 out 33 MRSA isolates. All MRSA isolates succeeded in forming biofilm; however, three (0.09%), 23 (69.69%), and seven (21.21%) isolates formed weak,moderate, andstrong biofilm, respectively. Gentamicin at sub MIC reduced the intensity of biofilm and the AFM confirmed this finding. In conclusion, very weak correlation linked the biofilm formation capacity and isolate MIC. On the other hand, possession of hemolysin genes seems has no correlation with biofilm formation. Nevertheless, gentamicin at sub MIC reduced the intensity of MRSA biofilm.

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Publication Date
Sat Feb 27 2021
Journal Name
Iraqi Journal Of Science
Application of Velocity Analysis Picking for 2D Seismic Data Processing in West An-Najaf Are

     In the current study, 2D seismic data in west An-Najaf (WN-36 line) were received after many steps of processing by Oil Exploration Company in 2018. Surface Consistent Amplitude Compensation (SCAC) was applied on the seismic data. The processing sequence in our study started by sorting data in a common mid-point (CMP) gather, in order to apply the velocity analysis using Interactive Velocity Analysis Application (INVA) with Omega system. Semblance of velocity was prepared to preform normal move-out (NMO) vs. Time. Accurate root mean square velocity (VRMS) was selected, which was controlled by flatness of the primary events. The resultant seismic velocity section for the study area shows that the veloci

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Publication Date
Thu Jun 01 2017
Journal Name
Journal Of Economics And Administrative Sciences
Controller work specificity and their impact on performance (Applied Study in the financial oversight bodies)

The research aims to a statement of specificity of the Controller of (academic achievement, specialty, job title, length of service, Gender) and its impact on performance, Through a proposed appraisal form includes three main axes and each axis including several specialized elements in the supervisory work in form (check list). as is the importance of research to enable officials of oversight bodies financial identify and diagnose performance Controller through what has this observer of the process of scientific properties when performing supervisory work. Be summarized problem of the research that the lack of regulatory institution with the Controller interesting in terms of the necessary characteristics and requirements and inv

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Publication Date
Sun Mar 01 2009
Journal Name
Baghdad Science Journal
Study of ELISA and antibiotic sensitivity test for Salmonella enteritidis as experimental infection in mice

Salmonella enteritidis one of more important as epidemiological bacteria between other salmonella types. It is very important pathologically that cause food poising and gastrointestinal tract infections. This study includes some of immunological changes that appear by ELISA test and antibiotic sensitivity test against these bacteria in mice. ELISA test results appears high immunological response happen after 3 days of inoculation, mean titration readings beginning 0.198 and the maximum mean titration after 15 days of inoculation 1.538 and begin to decrease after this time slowly to remain about 0.297 after 40 days of inoculation. An antibiotics sensitivity test result appears, this bacteria sensitive to Chloramphenicol, Ceftriaxone,

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Publication Date
Mon May 31 2021
Journal Name
Iraqi Journal Of Science
Suggesting Multipath Routing and Dividing the Zone to Process Communication Failure in Ad Hoc Networks

Ad hoc networks are characterized by ease of setup, low costs, and frequent use in the corporate world. They ensure safety to the user and maintain the confidentiality of the information circulated. They also allow the user to address the cases of communication failure in areas subject to destruction of communication infrastructure. The proposed protocols in the ad hoc networks often build only one path to achieve communication between the nodes, due to the restrictions of battery run out and the movement of the nodes. This connection is often subject to a failure within a certain range. Thus, multiple alternate paths in ad hoc networks use a solution to failing node communications. In addition, when looking at the situation where interf

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Publication Date
Tue Dec 01 2020
Journal Name
Journal Of Engineering
A Case Study of Bus Line Passenger Volumes of Bakrajo Bus Lines in Sulaimani City

Transit agencies constantly need information about system operations and passengers to support their regular scheduling and operation planning processes. The lack of these processes and cultural motivations to use public transportations contributes enormously to the reliance on the private cars rather than public transportation, resulting in traffic congestions. The traffic congestions occur mainly during peak hours and the accidents happening as a result of road accidents and construction works.  This study investigates the effects of weekday and weekend travel variability on peak hours of the passenger flow distribution on bus lines, which can effectively reflect the degree of traffic congestion. A study of passen

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Publication Date
Wed Feb 02 2022
Journal Name
Iraqi Journal Of Science
A chemotaxonomical Study of Four Species Belonging the Genus Arabis L. Cruciferae (Brassicacae) in Iraq

This study has been focused on the detection of phenolic compound in aerial parts (stem and leaves)of the species belonging the genus Arabis L. A.Caucasica Willd, A.Sagittata (Bertol)DC, A.Aucheri Boiss, A.nova Vill of family cruciferou (Brassicaceae) which were collected during field trips of the districted of Iraq. Phenolic compounds have been identified by using High Performance Liquid Chromatography (HPLC). Nine standard compounds used for comparison five of them flavonoids ( Rutin, Quercetin, kaempferol, Luteolin and Apigenin) and the other phenolic acid ( Chlorogenic acid, Caffeic acid, Ferulic acid and Rosmarinic acid) , results showed that species vary in containing phenolic compound which can be counted as a taxonomic evidence s

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Publication Date
Sun Apr 30 2023
Journal Name
Iraqi Journal Of Science
Batch and Flow Injection Spectrophotometric Determination of Tetracycline Hydrochloride and Doxycycline Hyclate in Pharmaceutical Preparations

New, simple and accurate batch and flow injection spectrophotometric
methods have been developed for the determinationsof tetracycline
hydrochloride (TCH) and doxycycline hyclate (DCH) in pharmaceutical
preparations. The methods are based on diazotization of
metchlopramide and coupling reaction with either TCH or DCH in alkaline
medium to form yellow–orange water soluble dye with absorption maxima
at 414 and 436 nm for TCH and DCH, respectively. A graphs of absorbance
versus concentration show that Beer’s law was obeyed over the
concentration ranges of 1 –52 μgmL-1 TCH and DCH for batch method and
of 8 – 240 μg mL-1 TCH and 5 – 350 μgmL-1 DCH for FIA method. The
limits of detection in batchmetho

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Publication Date
Mon Jan 28 2019
Journal Name
Iraqi Journal Of Science
Relation Between Aerobic Bacteria, IFN-γ, TNF-α and Miscarriage in Sample of Iraqi Women

Pregnancy   is one of the cases that lead to immune compression, therefore the women are exposed to different types of infections and may sequel to miscarriage. Toxoplasma gondii and urinary tract infection (UTI) are the most prevalent infectious agents in human and  have a worldwide distribution. IFN-γ and TNF-α are pro inflammatory cytokine play important role in miscarriage. This study focuses on the determination, type of aerobic bacteria , concentration of IFN-γ and TNF-α among (75) abortive women and  their relation with miscarriages  . The results indicated that S.aures was the most prevalence bacteria isolated from  aborted women infected with UTI or  compensation with toxo

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Publication Date
Wed May 25 2022
Journal Name
Iraqi Journal Of Science
Determination of the Level of IL-6 and Vaspin in Hyperthyroid Patients Treated with Carbimazole

      The level of adipokines (interleukin-6 and vaspin) in hyperthyroid patients treated with carbimazole drug was determined. Eighty-five male and female participated in this study, with mean age from 20-70 years. They included: negative control group of thirty euthyroid persons represented group 1, twenty-five hyperthyroid patient (positive control) included in the second group and thirty hyperthyroid patients treated with carbimazole drug for one year or less comprised the third group. By vein puncture, blood was collected, and serum was isolated and preserved at –20 C. Adipokines (interleukin-6 and vaspin) were estimated by using ELISA method. The results demonstrated that the the increase of IL-6 level was highly significant

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Publication Date
Thu Sep 01 2011
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
The Role of higher Education Institutes in restricting Administrative and Financial Corruption: An Analytic Reading

Corruption, in all its categories and forms, is regarded as the nowadays virus which has greatly spread in most institutes and society, a matter that cause a great waste of resources.

      According to the reports of international transparency Institute, Iraq is regarded as one of the greatest countries in corruption.

      Regardless of the reasons and forms of corruption, the retreat in work – values and ethics are the main reasons behind that.

      Being the main source of providing qualified staff "educators" for the working market, the high education institutes face great challenges in standing against corruption inside and outside

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