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Molecular diagnosis of Chlamydia trachomatis in infertile Iraqi women using Real time-PCR and comparison with other methods
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Chlamydia trachomatis is the most common of negative gram bacteria that cause sexually transmitted diseases. It affects the reproductive system in women, not the symptoms of the disease, but the most serious is the long-term effects of the reproductive system.. out of 100 women were attending different hospitals in Baghdad included the Gynaecology Departments of Women Health Center at Al-Elwyia Obstetrics Hospital . Ibn Al balady Maternity and Children's Hospital , Kamal al-Samarrai hospital Fertility Center infertility treatment and In Vitro Fertilization ( IVF ) (20 control and 80 women with infertility) DNA was extracted from the Endocervical Swabs of all infertili women, to investigate the bacteria by using Real time -PCR technique and another swab were subjected to direct Rapid Test . The venous blood sample was obtained, serum were diagnosis by Anti- Chlamydia trachomitis Chlamydia trachomitis IgG ELISA.
The result of the Real time-PCR revealed was significant associated with infertility women (OR= 0.733; 13.75%, P= 0.041), among 80 infertili women, while was no significant diagnosis by ELISA (OR= 0.063; 2.50%, p= 0.095). and no significant associated with Rapid Test .
In summary Real time-PCR has the possibility to contribute in the primary screening for infertility in women.

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Publication Date
Fri Sep 30 2022
Journal Name
Journal Of Economics And Administrative Sciences
Comparison of Some Methods for Estimating the Survival Function and Failure Rate for the Exponentiated Expanded Power Function Distribution
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     We have presented the distribution of the exponentiated expanded power function (EEPF) with four parameters, where this distribution was created by the exponentiated expanded method created by the scientist Gupta to expand the exponential distribution by adding a new shape parameter to the cumulative function of the distribution, resulting in a new distribution, and this method is characterized by obtaining a distribution that belongs for the exponential family. We also obtained a function of survival rate and failure rate for this distribution, where some mathematical properties were derived, then we used the method of maximum likelihood (ML) and method least squares developed  (LSD)

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Publication Date
Sun Jul 02 2023
Journal Name
Iraqi Journal Of Science
RT PCR Detection and Propagation of Respiratory Syncytial Virus in Human Lung Carcinoma Cells (A549) cell line
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In this study negative result of real-time reverse transcription-QPCR (RT-PCR) assay
tests of Influenza virus of nasal screetion and throat swap samples of Iraqi patients
hospitalized with signs and symptoms of an upper respiratory tract infection in Central
Republic Health Laboratory in Iraq were tested for Respiratory Syncytial Virus
infection by RT PCR .Positive samples was 4 out 0f 20 were used .Viral isolation was
done on a monolayer of 70-80% confluent Human Lung Carcinoma Cells (A549) cell
line and incubated at 33ºC for 4 days .Syncytia was observed in 3 positive samples.

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Publication Date
Sun May 12 2019
Journal Name
Journal Of The Faculty Of Medicine-baghdad
Level of follicular fluid vitamin D and embryo quality in a sample of Iraqi women undergoing IVF
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Publication Date
Thu Mar 30 2023
Journal Name
Iraqi Journal Of Science
Detection of Cytomegalovirus, Rubella virus, and IL-2 Levels in a Sample of Recurrently Aborted Iraqi Women
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The present study aimed to investigate CMV and Rubella virus as a causative agent of recurrent abortion, while the IL-2 levels were estimated as immune parameter during pregnancy period. A total of 63 blood samples were collected from recurrently aborted women, control non-pregnant women and control pregnant women. The results recorded 72.09 % CMV positive aborted women and 27.91 % Rubella virus positive aborted women. Levels of IL-2 were (437.03 ± 38.02) pg/ ml in first group, (390.51± 63.56) pg/ ml in second group, (32.98 ±15.12) pg/ ml in control group non pregnant women and (118.63 ± 24.81) pg/ ml in control pregnant women. High IL-2 levels in all studied women indicate presence of a factor affecting the immune system other than

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Publication Date
Sat Aug 17 2024
Journal Name
Al-rafidain Journal Of Medical Sciences ( Issn 2789-3219 )
Analysis of Follicular Fluid Fatty Acids in Iraqi Women Undergoing Intracytoplasmic Sperm Injection
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Background: Oocytes are susceptible to alterations in the various fatty acid contents of follicular fluid (FF), which may influence maturation and embryogenesis. Different fatty acids exert various effects on intracytoplasmic sperm injection (ICSI), which needs further studies to uncover the involved mechanisms. Objectives: To assess FF fatty acids in women undergoing ICSI and to correlate them with ICSI parameters, namely the total count of aspirated oocytes, oocyte maturation rate, fertilization rate and percentage of good-quality embryos. Methods: Fifty women undergoing ICSI were enrolled in this cross-sectional study. FF samples were collected during oocyte retrieval and were analyzed for fatty acids using gas chromatography. Fa

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Publication Date
Sat Aug 17 2024
Journal Name
Al-rafidain Journal Of Medical Sciences ( Issn 2789-3219 )
Analysis of Follicular Fluid Fatty Acids in Iraqi Women Undergoing Intracytoplasmic Sperm Injection
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Background: Oocytes are susceptible to alterations in the various fatty acid contents of follicular fluid (FF), which may influence maturation and embryogenesis. Different fatty acids exert various effects on intracytoplasmic sperm injection (ICSI), which needs further studies to uncover the involved mechanisms. Objectives: To assess FF fatty acids in women undergoing ICSI and to correlate them with ICSI parameters, namely the total count of aspirated oocytes, oocyte maturation rate, fertilization rate and percentage of good-quality embryos. Methods: Fifty women undergoing ICSI were enrolled in this cross-sectional study. FF samples were collected during oocyte retrieval and were analyzed for fatty acids using gas chromatography. Fa

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Publication Date
Fri Nov 24 2023
Journal Name
Iraqi Journal Of Science
Species Identification and Strain Differentiation of Dermatophyte by (GACA)4-Primer based PCR
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Dermatophytes are a group of morphologically and physiologically related molds some of which cause well defined infections: dermatophytoses (tineas or ringworm). The present study aims at identification of dermatophytes species and varieties from patients in Wasit province-Iraq using molecular approach based PCR fingerprint.
The short oligonucleotide (GACA)4 is a microsatellite primer was used in this study for identification of dermatophyte isolates. The results identified different species and varieties among dermatophytes. The numbers of resulting PCR bands ranged from 1 to 4 (size range, 600bp to 1600bp) for each species. The resulting patterns were distinct for Trichophyton and Microsporum species and varieties.
Trichophyton s

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Publication Date
Tue Jan 01 2019
Journal Name
International Journal Of Agricultural And Statistical Sciences,
The comparison of several methods for calculating the degree of heritability and calculating the number of genes II. Yield components
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Scopus (3)
Scopus
Publication Date
Wed Dec 01 2021
Journal Name
International Journal Bioautomation
Model for Prediction of the Weight and Height Measurements of Patients with Disabilities for Diagnosis and Therapy
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Background: Accurate measurement of a patient’s height and weight is an essential part of diagnosis and therapy, but there is some controversy as to how to calculate the height and weight of patients with disabilities. Objective: This study aims to use anthropometric measurements (arm span, length of leg, chest circumference, and waist circumference) to find a model (alternatives) that can allow the calculation of the height and the body weight of patients with disabilities. Additionally, a model for the prediction of weight and height measurements of patients with disabilities was established. Method: Four hander patients aged 20-80 years were enrolled in this study and divided into two groups, 210 (52.5%) male and 190 (47.5%) fe

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Publication Date
Sat Mar 01 2014
Journal Name
Glob. J. Mol. Evol. Genom
Genetic diversity and relationships among Verbascum species in Iraq by RAPD-PCR technique
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