The objective of the present study was to investigate the effect of body mass index (BMI) on semen parameters, level of sex hormone and sperm DNA integrity. Semen samples were collected from (85) infertile men and (40) healthy fertile men with range of age (38.191 ± 0.84) years during their attendance at High Institute of Infertility Diagnosis and ART, Al-Nahrain University from March to June 2016. Semen samples were obtained by masturbation after 72 hours of abstinence. Seminal fluid analyses included semen volume, sperm concentration, percent sperm motility, percent sperm morphology, and sperm chromatin integrity DNA fragmentation index (DFI]). Serum samples were collected from each subject for determination the level of Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Prolactin (PRL), and Testosterone by ELISA method. The results revealed a highly significant (P≤ 0.01) increase in BMI and immotile sperm (%), and significant(P≤ 0.05) increase in semen liquefaction time, non-progressive motility (%), round cells counts and sperm DNA fragmentation in infertile men as compared to control group, while there was a highly significant (P≤ 0.01) decrease in progressive motility (%), and a significant (P< 0.05) decrease in the sperm concentration, sperm motility (%) and normal sperm morphology (%). The results showed statistically significant (P< 0.05) positive correlations between body mass index and sperm motility, progressive motility, non-progressive motility, immotile sperm, normal sperm morphology and sperm DNA fragmentation. No significant correlations were observed between body mass index and semen liquefaction time, semen PH, sperm concentration, round cells counts and age. In respect with level of serum hormones a significant (P< 0.05) decrease in level of FSH ,LH and testosterone was found ,while the level of prolactin showed a significant (P< 0.05) increase in infertile men when compared with control group. Significant (P< 0.05) negative correlation was observed between body mass index and serum level of prolactin and testosterone, while non-significant correlations were observed between body mass index and serum level of FSH and LH. In conclusion, this study has shown that body mass index has major effect on semen characteristics and sex hormones.
Background: Candida tropicalis is one of the most causes of vulvovaginal candidiasis (VVC) in women. Systemic candidiasis and candidemia may also occur in pregnancies. Objective: This study was carried out to detect and isolate of this yeast from aborted placenta, which may cause severe complications such as spontaneous abortion. Materials and methods: Fresh aborted placenta were collected and washed by normal saline to remove the blood. Then, cut it into portions and place it in test tube containing 5 ml of normal saline. Finally, shake for 10 minutes, after that, cultured for microbial isolation. Isolation and detection were done by some conventional methods with Api candida and CHROMagar. Results: The results showed that four iso
... Show MoreRosemary (Rosmarinus officinalis L.) is one of the most economically important species of the family Lamiaceae. Rosemary extract was examined by applying 2.2-diphenyl-1- picrylhydrazyl (DPPH) radical-scavenging assays. The result proved that rosemary extract had a higher antioxidant activity by absorption at a wavelength of 517a nm by using three different concentrations (0.5, 1.0 and 3) mg/ml which performed the absorbance at (2. 314, 0. 211 and 0.296) nm in comparison with control (21.8, 92.2 and 90) nm respectively. Results obtained using chemical detection of the phytochemicals indicated the presence of flavonoids, phenols, saponins, Steroids and cardiac in rosemary water extract. Water extracts of R. officinalis leaves were inves
... Show MoreThe present study combines UV-Vis spectrophotometry and dispersive liquid-liquid microextraction (DLLME) for the preconcentration and determination of trace level clidinium bromide (Clid) in pharmaceutical preparation and real samples. The method is based on ion-pair formation between Clid and bromocresol green in aqueous solution using citrate buffer (pH = 3). The colored product was first extracted using a mixture of 800 µL acetonitrile and 300 µL chloroform solvents. Then, a spectrophotometric measurement of sediment phase was performed at λ = 420 nm. The important parameters affecting the efficiency of DLLME were optimized. Under the optimum conditions, the calibration graphs of standard -1 (Std.), drug, urine and serum were ranged
... Show MoreThe rapid spread of novel coronavirus disease(COVID19) throughout the world without availablespecific treatment or vaccine necessitates alternativeoptions to contain the disease. Historically, childrenand pregnant women were considered high-riskpopulation of infectious diseases but rarely have beenspotlighted nowadays in the regular COVID-19updates, may be due to low global rates of incidence,morbidity, and mortality. However, complications didoccur in these subjects affected by COVID-19. Weaimed to explore the latest updates ofimmunotherapeutic perspectives of COVID-19patients in general population and some added detailsregarding pediatric and obstetrical practice.Immune system boosting strategy is one of therecently emerging issue
... Show MoreIn this study, the development of an indirect spectrophotometric method for the determination of folic acid in pure and pharmaceutical preparations is described. The method is based on the oxidation of pyrocatechol with iron (III) in an acidic medium, followed by the reaction with folic acid (FA) to produce a stable, water-soluble orange compound with maximum absorption at 350 nm versus the blank reagent. The complex of charge transfer was studied under optimal conditions; the titration graph was linear over the range of 0.5-25 μg/mL with a relative error of 1.2-2.8 and a relative standard deviation of 2.43-1.45 depending on the concentration level.
Morphological and histological study was conducted to examine the structure of prosencephalon (Cerebrum) in Columba oenas (Linnaeus, 1758). Thirteen animals of C. oenas were used in the present study. The brains were excised, fixed and recessed for the preparation of microscope slides and used hematoxylin-eosin stain, methylene blue and Bielschowsky’s stain. The result of morphological study showed that the cerebrum is apportion of the prosencephalon and the largest part of the brain and the dorsal surface was smooth and convex and composed of two cerebral hemispheres. The cerebral hemisphere contains the cavity which called lateral ventricle. The hemispheres were connected by the anterior commissure. Histologyically the cerebral hemisphe
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