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ijs-5789
Detection of Efflux Pumps Gene and Relation with Antibiotics Resistance in Uropathogenic Escherichia Coli (UPEC) Isolated from Patients with Cystitis
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Background: In recent years, the multidrug-resistant (MDR) Escherichia coli has increased in urinary tract infections (UTIs). One of the highly distributed chromosomally encoded traits of resistance is efflux pump. The current study aimed to investigate the most common members of 5 classes of efflux pumps among uropathogenic E. coil isolates.

Methodology: E. coli isolates were isolated using conventional bacteriology tests   and confirmed by the uidA gene. An antibiotic susceptibility test has been done against 25 antibiotics using disc diffusion method. Efflux pump genes have been examined via polymerase chain reaction. Biofilm formation was investigated by a 96-well polystyrene microtiter plate.

Results: In total, 50 E. coli isolates were collected from UTIs. All isolates were resistant to amoxicillin, ceftazidime, and cefotaxime, while all isolates were sensitive to imipenem.  Also, 19 (38%) E. coli isolates were non-MDR, while 31 (62%) were MDR.  The frequency rates of different efflux pump genes ranged from 66% to 100%. The biofilm formation results indicated that 98% of isolates were biofilm former while 2% were non-biofilm. In total, 60% was weak biofilm, 24% was moderate, and 14% was strong biofilm former.

Conclusion: The current study concludes that all efflux pumps may be highly associated with resistance to amoxicillin, cefotaxime, and ceftazidime and moderately associated with cefepime, ceftriaxone, and cefixime and may be unrelated to the resistance of other studied antibiotics or the concentration of these antibiotics inadequate to induce the expression of these pumps. Additionally, biofilm formation was highly related to the presence of study pumps.

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Publication Date
Sun Apr 03 2005
Journal Name
Journal Of The Faculty Of Medicine Baghdad
THE CATHETERIZATION AND ANGIOGRAPHIC VARIATIONS IN IRAQI PATIENTS WITH TETRALOGY OF FALLOT
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Background: Tetralogy of Fallot (TOF) is the most common form of cyanotic congenital heart disease and the catheterization and angiography still considered (in most centers) as essential preoperative diagnostic step. This retrospective aimed at evaluating the catheterization and angiographic finding in our Iraqi patients with diagnosis of TOF
Patients and methods: The catheterization and angiographic study of200 patients with TOF-' referred to Ibn Al-Bitar Cardiac Center had been reviewed.
Results: There were 126 males and 74 females and their ages ranged from 11 months to 37 years. The catheterization data showed that 88% of the patients had equal LV and RV pressure. The evaluation of pulmonary artery pressure showed that all pati

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Publication Date
Sun Jun 30 2013
Journal Name
Al-kindy College Medical Journal
Cognitive Effects of Tumor and Surgical Treatment in Patients with Cerebral Gliomas
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Background: Quality of life in brain tumor patients is an emerging issue and has prompted neurosurgeons to recon¬sider the need for cognitive assessment in the course of treatment. To date there has been a lack of comprehensive neuropsychological assessment performed preoperatively and in the acute postoperative period in our hospitals.Objectives: to establish the effects of tumors and their surgical treatment, from a neuropsychological perspective, on cognitive functioning in patients with cerebral Gliomas. Methods: This is a prospective study conducted in the Neurosurgical Hospital in Baghdad, Iraq, during the period from January 1999 to January 2001. Any patient admitted during the period of the study with clinical history, signs, sy

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Publication Date
Sun Oct 02 2016
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Prevalence of Entamoeba Histolytica infection in patients with colitis (Ulcerative and Infective)
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Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon that often extends to involve the remainder of the large intestine in a continuous manner. Intestinal amoebiasis can present as a chronic, dysenteric syndrome of diarrhea, weight loss, and abdominal pain that can last for years and mimic ulcerative colitis.
Objective: Studying the prevalence of E..histolytica by using serological method ELISA for their detection, Investigate the possible role of E.histolytica as a cause of blood, diarrhea in non-ulcerative colitis group &Comparison between serological detection of E.histolytica and stool examination.
Patients and Methods: Retrospective and prospective study was conducted

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Publication Date
Sat Jan 11 2025
Journal Name
Journal Of Baghdad College Of Dentistry
Assessment of serum and salivary malondialdehyde in patients with oral lichen planus
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Background: The aim of this study was to evaluate the expression of fibroblast growth factor-2 and Heparanase in salivary pleomorphic adenoma, and to correlate the two studied markers with each other and with clinicopathological parameters including: age, sex, tumor site and histopathological presentation. Methods: Sections of twenty five formalin-fixed paraffin embedded tissue blocks specimens of salivary pleomorphic adenoma were immunostained using monoclonal antibodies (Fibroblast growth factor-2 and Heparanase) to assess their expression in this tumor. Results: The expression of fibroblast growth factor-2 and Heparanase were positive in all pleomorphic adenoma cases (100%). The positive expression of fibroblast growth factor-2 was signi

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Publication Date
Mon Jul 01 2024
Journal Name
Indian Journal Of Clinical Biochemistry
Evaluation of Bone Turnover Markers in Patients with Acute and Chronic Leukemia
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Publication Date
Mon Nov 30 2020
Journal Name
International Journal Of Civil Engineering
Adsorption of Meropenem Antibiotics from Aqueous Solutions on Multi-Walled Carbon Nanotube
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Pharmaceutical-instigated pollution is a major concern, especially in relation to aquatic environments and drugs such as meropenem antibiotics. Adsorbents, such as multi-walled carbon nanotubes, offer potential as means of removing polluting meropenem antibiotics and other similar compounds from water. In order to evaluate the effectiveness of multi-walled carbon nanotubes in this capacity, various experimental parameters, including contact time, initial concentration, pH, temperature and the dose of adsorbent have been investigated. The Langmuir and the Freundlich isotherm models have been used. The data obtained using a modified Langmuir model have been consistent with the experimental ones; the best pH value has been obtained to have the

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Publication Date
Sun Oct 02 2016
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Molecular Classification of Iraqi Breast Cancer Patients and Its Correlation with Patients’ Profile
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Background: Breast cancer is a complex, multifaceted disease encompassing a great variety of entities that show considerable variation in clinical, morphological and molecular attributes.
Objective: The aim of this study to evaluate patients’ molecular profile (Estrogen receptor, Progesterone receptor, HER2/neu and Ki-67).
Patients & Methods: This is a cross-sectional descriptive study was done in Baghdad oncology teaching hospital from December 2015 to April 2016, carried on 100 breast cancer female patients with their age range from 27 to 73 years old and with their histopathology reports and (IHC) results.
Results: The highest incidence of breast cancer among patients in 5th (40-49 years) and 6th (50-59 years) decades o

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Publication Date
Tue Jun 01 2021
Journal Name
Al-anbar Medical Journal
Evaluation of Antimicrobial Activity of Plants Extract Against Bacterial Pathogens Isolated from Urinary Tract Infection among Males Patients
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Urinary tract infection (UTI) is a considerable problem aecting the health of people each year. It is caused by various Gram-positive (G+ve) and Gram-negative (G-ve) pathogens. It is an important illness in the world aecting all age groups across their life span. Objectives: To identify the most common aerobic bacteria that cause UTIs and their antibiotic susceptibility and antimicrobial activity of plant extracts of the males' patients. Materials and methods: The study involved 35 midstream urine samples from the male students (University of Baghdad, Baghdad, Iraq) with suspicious symptoms of UTI, during the period from January-March 2018. Each urine sample was cultured rst on Mannitol Salt Agar and MacConkey agar plates to dierentiate

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Publication Date
Tue Jun 01 2021
Journal Name
Al- Anbar Medical Journal
Evaluation of Antimicrobial Activity of Plants Extract Against Bacterial Pathogens Isolated from Urinary Tract Infection among Males Patients
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Publication Date
Sat Sep 15 2018
Journal Name
Journal Of Baghdad College Of Dentistry
Evaluation of Hematocrit Level, Red Blood Cells and White Blood Cells Counts in Blood from Patients with Different Severities of Periodontal Diseases
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Background: Anemia of chronic disease (ACD) occurs in the presence of chronic infection, inflammatory conditions or neoplastic conditions despite of adequate iron and vitamins storage. Gingivitis is the inflammation of the gingiva, periodontitis is the inflammation in the periodontium that extend deeper with loss of connective tissue attachment and supporting bone. The main pathogenesis of periodontal diseases and ACD is immune activation. Aims of study: Determine and compare the clinical periodontal parameters (plaque index (PLI), gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD) and clinical attachment level (CAL)). Evaluate the hematocrit (Hct) level, red blood cells (RBCs) count and white blood cells (WBCs) c

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