Preferred Language
Articles
/
ijs-574
The Molecular Detection of HPV Infection in samples of Iraqi Women with Abnormal cervical Smears
...Show More Authors

Human papillomavirus (HPV) types 16 and 18 cause almost 70% of cervical cancer cases worldwide. Recently, testing for high-risk HPV types have been adopted by clinical practices for the early detection of cervical cancer in conjunction with cytology tests.

Cervical swab samples were collected at the Outpatient Gynecology department of Baghdad Teaching Hospital. These samples consisted of a patient group of 50 samples, and a healthy control group of 10 samples. A papanicolaou test (abbreviated as a Pap test) was also performed for each woman to examine the epithelial cells of both the endocervix and the upper vaginal region. Total DNA (genomic, mitochondrial, and viral) was extracted from cervical swab samples for molecular studies.

HPV DNA testing was first done by using Real-Time PCR technology to target the L1 region of HR-HPV with specifically designed primers. This was followed by using AmpliSens kit for specific detection and genotyping of HPV16 and 18 with multiplex Real-Time PCR.

The results of RT-PCR detection revealed that out of 16 samples detected with high-risk HPV, 5 samples were shown to be infected with HPV-16 and 5 samples were shown to be infected with HPV-18. These results show a significant relationship between the histological outcome of the patient and persistent HPV infection.

View Publication Preview PDF
Quick Preview PDF
Publication Date
Mon Oct 01 2007
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Significance Of Cervical Cytology Screening (Pap Smear) In The Detection Of Precancerous Cervical Lesions
...Show More Authors

Summary:
Background: The incidence of cancer of the cervix is comparatively low in Iraq, as in most other Islamic countries. Pre invasive lesions of the uterine cervix can be detected by Pap smears in their preclinical course, a fact which documents the significant role of cervical cytology in gynecological practice.
Objective: To emphasize the role of cervical cytopathology (Pap smears) in the detection and follow up of various cervical lesions that may predispose to cervical carcinoma with special emphasis on Cervical Intraepithelial Neoplastic (CIN) conditions or dysplasias.
Patients and Methods: This retrospective study M’as performed on 1500 women aged 20 years and above who attended the private clinical laboratory of th

... Show More
View Publication Preview PDF
Crossref
Publication Date
Wed Jan 01 2020
Journal Name
Biochemical And Cellular Archives
Expression of ebv latent membrane protein 1 (LMP1) in Iraqi women with cervical carcinoma
...Show More Authors

The design of this paper is to find the possible correlation of Epstein Barr virus infection ina group of Iraqi women with cervical carcinoma though detection of Latent Membrane Protein 1 (LMP1) in these cervical tissues. Paraffinized blocks of two groups were included. The first sample of 30 cervical carcinomatous tissues and 15 biopsies from an apparently normal cervical tissues. All the samples were sectioned on a positive charged slides with 4 mm – thickness then submitted for immunohistochemical (IHC) staining to detect viral LMP1 expression. Sixty three percentage (19 out of 30) of the studies group showed positive overexpression as shown in with a significant association of the expression with cervical cancer with a significant ass

... Show More
Publication Date
Mon Apr 01 2013
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Molecular Study of Human Mammary Tumor Virus in Iraqi Women with Breast Cancer
...Show More Authors

Background: Earlier reports related the presence of Mouse Mammary Tumor Virus -like gene sequences to human breast carcinoma. Mouse Mammary Tumor Virus -like gene is a retrovirus, namely, a virus containing reverse transcriptase which transcript its RNA to DNA in a process that enables genetic material from the retrovirus to become a part of the genes of an infected cell permanently. The virus that found in women was designated as Human Mammary Tumor Virus by the authors, who have investigated the presence of Human Mammary Tumor Virus sequences in a many human breast tissues and in many countries.

Objectives: Detect HMTV genome in Iraqi women of breast cancer.

Patients and Methods

... Show More
View Publication Preview PDF
Crossref
Publication Date
Sat Apr 15 2023
Journal Name
Iraqi Journal Of Science
Detection of Respiratory Syncytial Virus infection in Clinical Samples Using Immunofluorescence Test
...Show More Authors

Current study aimed to investigate the Respiratory Syncytial Virus (RSV) in nasal
secretion and throat swab samples of hospitalized patients with symptoms of
respiratory tract infection using Immunofluorescence test. Previously these samples
were tested for Respiratory Syncytial Virus infection by reverse transcriptase-PCR
assay. The positive samples were tested by Immunofluorescence assay in monolayer
confluent of Hep 2 cell line. The results showed that the positive samples using the
RT-PCR test were positive in this test. These results reveal that Immunofluorescence
test is sensitive method for detection the infection with RSV.

View Publication Preview PDF
Publication Date
Fri Jun 16 2023
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
The Isolation and Identification of Salmosamonella typhi from clinical samples with molecular detection of O-antigen encoded genes
...Show More Authors

Abstract

Antibiotic treatment of  S.typhi is difficult as compared to treatment of acute infection. Antibiotic resistance carried against S.typhi  by using 6 kinds of antibiotics from  different classes, their results  showed that all isolates were  high resistance to Ampicillin (99%), Gentamicin (98%), Amikacin (79%) and   less  resistances  Trimethoprim (55%) ,  Imipenem (60%)  and Ceftriaxone(66%)  .

The present study focused on the molecular detection of Wzx flippase, Wzy polymerase genes in some Salmonella typhi isolates, Samples were collected from typhoid patients by classical lab work. Antibiotics susceptibilit

... Show More
View Publication Preview PDF
Scopus Crossref
Publication Date
Sun Jul 04 2010
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Epidemiological characteristics of women with cervical erosion in Al-Sader city, Baghdad, Iraq.
...Show More Authors

Background: Cervical erosion (CE) is a common finding on routine pelvic examination during the fertile years. Published articles on CE in Iraq are scarce; therefore this study was carried out.
Methods: A total of 100 non pregnant women had CE attending a private clinic in Al-Sader city, Baghdad, was included in this study. Pelvic examination was done; demographic data and gynaecological history were obtained.
Results: The age of women was 27.1 ± 5.9 years with 7.3 ±2.9 educational years. Mucopurlent vaginal discharge was a complaint in 49% of women, and a watery vaginal discharge was a complaint in a similar percent. Thirty four of women were oral contraceptive users and 11% were using intrauterine c

... Show More
View Publication Preview PDF
Crossref
Publication Date
Thu Apr 28 2022
Journal Name
Iraqi Journal Of Science
Molecular and Bacteriological Detection of Some Bacterial Vaginosis Associate Bacteria in Women
...Show More Authors

Bacterial vaginosis (BV) is one of the most common genital infections among women in the childbearing age. Many novel, fastidious and uncultivated bacterial species are related with BV. These are called bacterial vaginosis associated bacteria (BVAB), present in trace amount and have a significant role in the infection. A total of 80 vaginal swabs were obtained from 80 pregnant and non-pregnant women. Samples were collected from different hospitals in Baghdad city and Al-Kut city.
Clinically, 60 sample among 80 were gave positive results depending on Nugent score and Amsel criteria ,the Bacteriologicall test showed the percentages of gram negative bacteria (E.coli ,K.pneumoniae, P.mirabilis, Ps.aeruginosaand A. baumanniiwere) were (38.

... Show More
View Publication Preview PDF
Publication Date
Fri Dec 30 2022
Journal Name
Iraqi Journal Of Science
Detection of FOXP-3 Expression in a Sample of Iraqi Cervical Cancer Patients Using Immunohistochemistry Technique
...Show More Authors

     This paper aims to find out if FOXP-3 was expressed in samples from Iraqi cervical cancer patients. Expression of FOXP-3 was detected in 55 cervical tissue samples by immunohistochemistry. Since thirty-five cases of aggressive cervical cancer were included, along with 20 normal samples used as controls. The nucleus and cytoplasm levels of FOXP-3 were counted, considering the ratio of positive cells and intensity. FOXP3 cytoplasmic staining was found in 27 out of 35 cases. Only 11 out of 35 samples displayed nuclear lymphocyte staining. Furthermore, four samples expressed this marker in both the nuclear and cytoplasm of the cervical cells. There is a highly significant difference in FOXP3 expression in the cytoplasm of

... Show More
View Publication Preview PDF
Scopus (2)
Crossref (1)
Scopus Crossref
Publication Date
Sat Jun 30 2018
Journal Name
International Journal Of Medical Research & Health Sciences
Assessment of the Healthy Women by Detection and Determination of Cells in Conventional Pap Stained Cervical Smear Images
...Show More Authors

Introduction: A Pap test can detect pre-cancerous and cancerous cells in the vagina and uterine cervix. Cervical cancer is the easiest gynecologic cancer to be prevented and diagnosed using regular screening tests and follow-up. This study aimed to estimate the cytological changes and the precancerous lesions using Pap smear test and visual inspection of the cervices of Iraqi women, and also to determine the possible relationship of this cancer with patients’ demographic characteristics. Methods: The study included 140 women aged (18-67) years old referred to the National Cancer Research Center (NCRC), Baghdad, Iraq, during the period 2011-2016. Both visual inspections of the uterine cervix and Papanicolaou smear screening were performed

... Show More
Preview PDF
Publication Date
Sun Jan 01 2023
Journal Name
Bionatura
Detection of lukf-pv gene in Staphylococcus aureus isolated from pregnant women with Urinary tract infection
...Show More Authors

Scopus Crossref