This study was aimed to detect weather copA gene(copper resistance gene ) presence in A.baumannii A92 genome(AbaR genomic islands). The full genomic sequence of A.baumannii A92 not published in NCBI genome similarity was detected between two strains so the sequence of A.baumanniiIS-116(Genebank-AMGF0100000.1 ) was used to design the primers that were used for amplify of copA gene of A.baumannii A92.
Two primers contain two sites for restriction enzymes (KpnI,XohI) and PWSK29 vector were used in the cloning, double digestion has been performed for vector and gene. Then the re-ligation was completed to form recombinant molecule,after that, transformation have been performed for the recombinant molecule by using chemical competent E.coli

Seventy of Klebsiella pneumoniae isolates had been collected from some Hospitals in Baghdad city from October to December 2017. The 70 isolates were taken from diverse clinical specimens. All K. pneumoniae isolates were identified based on API 20 E and Vitek2 compact system. Antibiotics sensitivity test was carried out toward 10 antibiotics using discs diffusion method. The level of antibiotics resistance was 81.42% for Ceftriaxone, whereas the low level of antibiotics resistance was 37.14% for Piperacillin. K. pneumoniae isolates were typed genotypically by using two different methods of amplification, multiplex-PCR and enterobacterial repetitive intergenic consensus (ERIC)-PCR typing methods. Results showed that out of 70 isolates, there

Background: Fibromyalgia is a functional disorder of growing importance characterized by chronic widespread musculoskeletal pain. It frequently affects women of child bearing age. Infertility is failure to achieve pregnancy within a 12 month period for sexually active individuals under 35 years of age and failure to conceive within a 6 months period for those over 35 years, which can have a major psychosocial impact on the affected person’s life.

Objectives: To estimate the prevalence of fibromyalgia among a sample of infertile women; and to study its relationship with age, length of marriage, duration and type of infertility.

Patients and Methods: A cross-sectional stu

Rice is a major staple food for more than two thirds of the world population. Pathogenesis-related proteins-10 (PR10) have a range of 154 to 163 amino acid with molecular weight ~ 17 kDa.  They are acidic and generally intracellular and cytosolic proteins accumulate in plants in response to biotic and abiotic stresses. In the present study, a PR10 gene and its corresponding protein were characterized in O. sativa, O.  barthii, O. glaberrima, O. glumipatula, O. meridionalis, O. nivara, O. rufipogon and  O. punctata. The results revealed a narrow range of variation at both DNA and protein levels in all examined species except O. glumipatula. The latter showed a relatively

Eight isolates of methicillin resistance Staphylococcus aureus(MRSA) (SA40,SA32,SA30,SA13,SA10,SA36,SA3 and SA7) with different resistance phenotypes  to macrolides , lincosamides and streptogramins Were used to detect theexpression of msrA, msrB, and linA/linA’genesby using  real time polymerase chain reaction before and after treatment with antibiotics (erythromycin , clarithromycin , clindamycin and lincomycin) calibrated with triosphosphateisomerase.There highst expression of these genes was after 18 hours. It was  an induction in the expression of msrA gene in isolates (SA40,SA32,SA30 and SA13) in presence of erythromycin,however,the  isolates showed reduction in expression l

A total of 41 patients with gastro duodenal symptoms (show signs of inflammation with or without duodenal ulcer) . 21 males (51.2%) and 20 female (48.8%) with an average age 0f  (20 – 80) years old under  going gastrointestinal endoscopy at Baghdad teaching hospital in internal disease clinical laboratory , between (February – June)  2009 . Biopsies specimen of antrum , gastric fundus ,& duodenal bulb were examined by the following methods (rapid urease test , Giemsa stain section to detect bacteria , & Haematoxilin and Eosin stained section for pathological study which are considered the gold standard methods , sera or plasma from these patients were tested by immunochromotography (ICM),serological m

Background: L. sativum, are traditionally used for the treatment of various diseases and thought to have medicinal value. Isolates from many part of the world is now multidrug resistant. Therefore, there is an urgent need to look for and test an alternative herbal drug.

Objective: The present study aimed to evaluate the antibacterial activity of L. Sativum seed extract against multi drug resistant (MDR) and sensitive Pseudomonas aeruginosa clinical isolates.

Subjects and Methods: An ethanolic and aqueous stock extracts were prepared from L.  sativum seed plant then serial dilutions were prepared and the obtained concentrations (50, 25, 12.5 and 6.2 mg/ml) were tested against 30 multidrug-resistan

Some children are suffering from introversion in kindergartens , so it must be confront and obviated by specialists because it will inevitably lead to unsatisfactory results reflect negatively on the individual and the community together . And the introvert child is a miserable child incapable of social interaction or give - and - take with colleagues , lack of integration of the child to life leads to the obstruction of  participation with his peers in various activities.     Introversion  causes a real emotional deficit to the children of kindergartens which limits of possibility of their intellectual development and mental growth and that because of their isolation and poor focus in education

This current research aims to identify the effectiveness of a training program in developing moral intelligence and mutual social confidence among middle school students. The researcher made a number of hypotheses for this purpose to achieve the goal of the research.                             

The researcher relied on the (Al Zawaida 2011) scale prepared according to Coles (1997), including (60) items, and the mutual social trust scale for (Nazmi 2001) based on Roter's theory including (38) items.               &nbsp