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The inhibitory effect of Trichoderma harzianum CA-07 crude extract against Trichophyton mentagrophyte and Microsporium canis
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The study aimed to identify Trichoderma harzianum isolates morphologically and by using PCR teqnique and evaluate the antifungal activity of T. harzianum CA-07  crude extract against Trichophyton mentagrophytes and Microsporium canis from patients with dermatophytosis. T.harzianum isolates were collected from the soil of Baghdad University gardens and they were identified depending of morphological features on plate and microscopic examination. The genomic DNA of T.harzianum isolate was extracted at a final concentration of (400-600) μg / 2-3 g of wet mycelium and at a purity of 1.6-1.8and DNA sample was amplified with each of universal primers (ITS-1& ITS-4) to be used for detection of Trichoderma species. The crude extract was extracted from T.harzianum strain CA-07 by ethyl acetate with finalyield of 4.8gm., then different crude extract concentrations (0.5, 1, 2 and 4 mg/ml) were used against the clinical pathogenic fungi (T.mentagrophyte and M.canis) using agar well diffusion method. The results of identification of Trichoderma harzianum isolates by using PCR confirmed that the isolate was T.harzianum strain CA-07,and their crude extract exhibited significantly high antifungal activity against T. mentagrophyte and M. canis with high growth inhibition zones(14, 12mm) respectively at the lower concentration (0.5mg/ml)of crude extract.

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Publication Date
Thu Dec 28 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Effect of Carthamus Tinctorius Safflower Aqueous Extract Against Nickel Chloride Induces Hematotoxicity and Immunotoxicity in Adult Male Rabbits
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  This study was designed to show, the role of Carthamus tinctorius safflower aqueous extract against toxicity of nickel chloride (NiCl2). Twenty male, rabbits were used and divided into four groups (with 5 rabbits in each group); group (control group) received normal diet, group II received orally 100mg/kg NiCl2 for six weeks, group III received 100mg/kg NiCl2 and 100mg/kg extract six weeks, group IV received 100mg/kg NiCl2 and 200mg/ kg extract six weeks. Hematological parameters showed (RBC (Red blood cells), Hb (Hemoglobin), PCV (Packed cells volume) decreased and WBC (White blood cells) increased) significant changes (P < 0.05) compared with control group. Immunological parameters (IgG, IgA and IgM increased) and oxidative s

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Publication Date
Tue Mar 14 2023
Journal Name
Iraqi Journal Of Science
Inhibitory Effect of Grapefruit Extracts on the Growth and Adhesion Ability of Staphylococcus aureus
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Ten isolates belong to the Staphylococcus bacteria from different clinical swabs were taken from patients in Ibn al-Nafis Hospital and Central Public Health laboratory, according to many morphological and biochemical tests that used to identify bacterial species S. aureus, the results showed that 8 isolates when investigated their ability to produce a slime layer using Congo red agar method the results showed that SA5 isolate was the best compared to other isolates through change the color of colonies to the pink and Congo red agar -colored Black.
When examining the inhibitory effect of grapefruit extracts in the growth of isolated bacteria SA5 S.aureus, results showed that the aqueous extract of the seeds at different concentrations

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Publication Date
Sat Dec 11 2021
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Evaluation of Synergistic Antibacterial Effect of Combined Scrophularia striata Extract and Antibiotics Against Pseudomonas aeruginosa and Methicillin -Resistant Staphylococcus aureus
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Scrophularia. striata from Scrophulariacea family has been used in Iranian folk medicine for the treatment of infectious diseases. In this study we evaluated the synergistic effect of S. striata   hydroalcoholic extract (SSE) and commercially available antibiotics against P. aeroginosa and Methicillin- resistant Staphylococcus aureus (MRSA). The resazurin-based microdilution method was used to determine the minimum inhibitory concentration (MIC) values of plan extract and standard antibiotics. The interaction between standard antibiotics and SSE was evaluated by using checkerboard method. The results of this study revealed that SSE enhance the antibacterial activity of antibiotics. The combin

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Publication Date
Sat Oct 01 2022
Journal Name
Iraqi Journal Of Veterinary Sciences
The histological and histochemical features of the esophagus in local breed dogs (Canis familiaris)
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Publication Date
Mon Aug 29 2016
Journal Name
World Journal Of Experimental Biosciences
Effect of sub-inhibitory and inhibitory concentrations of some antibiotics and rosemary essential oil (Rosmarinus officinalis L.) on biofilm formation of Klebsiella pneumoniae
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Klebsiella pneumoniae have an ability to form biofilm as one of strategies to persist and overcome host defenses. The study aims to evaluate the effectiveness of rosemary essential oil alone and in combination with some antibiotics against biofilm of K. pneumoniae isolated from urine. The antibiotics resistance pattern by disc diffusion method and minimal inhibitory concentration (MIC) of gentamicin, ciprofloxacin, amoxicillin, trimethoprim/ sulfame- thoxazole, cefotoxime and rosemary essential oil were determined. The ability to form biofilm as well as inhibition of biofilm formation of K. pneumoniae was performed. MICs 128, 0.25, 768, 64, 384 and 10 µg/ml were used. The effect of MIC and 1/2 MIC of antibiotics and rosemary essential oil

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Publication Date
Tue Jan 15 2019
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
INHIBITORY EFFECT OF THYMOL EXTRACTS ON ENTEROTOXINS PRODUCTION BY Staphylococcus aureus.: INHIBITORY EFFECT OF THYMOL EXTRACTS ON ENTEROTOXINS PRODUCTION BY Staphylococcus aureus.
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One hundred and eighty five urine samples were collected eight isolates (4.3%) were obtained and diagnosed as Staphylococcus aureus. Among 8 isolates, 5 (62.5%) S. aureus isolates were found to be enterotoxigenic, most of isolates produced at least two types of Staphylococcal enterotoxins (SEs). The production of enterotoxins in the presence or absence of Thymol extracts (aqueous and alcoholic) were estimated using a reversed passive latex agglutination (SET-RPLA) kit. The extracts reduced enterotoxin production compared with the control. Enterotoxin inhibition was observed for enterotoxin C production at minimal inhibitory concentrations (MIC) at 400 µg/ml, whereas production of enterotoxins A, B, and

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Publication Date
Sun Sep 07 2014
Journal Name
Baghdad Science Journal
The Aqueous Crude Extract of Carrot Seeds ( Daucus carota L.) and it Role in Fertility of Male Albino Mice
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This study was designed to test the effect of the treatment with aqueous extract of carrot seeds( Daucus carota) on fertility of male albino mice by effected on Body weight , testis weight ,secondary sex organs (prostate , seminal vesicle) and sperms properties .This experiment used (24) albino male mice aged(8-10)weeks. these animals were randomly divided into three groups contain two doses (200,400mgkg) were used from both extracts ,they were daily and orally given for (35)days ,and control group was given physiological saline. After the end of the experiment the animals were sacrificed after taken their body weights. The results were show: 1. A significant (p<0.05) increase in body weight and reproductive organs. 2

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Influence of alcoholic extract crude fenugreek seeds on fertility in male mice eggs
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These dust designed to identify the extent of the impact of alcohol Almstkhalss saponin from fenugreek seeds on fertility in male mice eggs by tracking some physiological changes and tela that may occur to some members of the device Altcatherthe

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Influence of alcoholic extract crude fenugreek seeds on fertility in male mice eggs
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Histologic changes were studied and physiological dosage crude alcoholic extract of seeds of the fenugreek plant for male mice eggs in different concentrations after oral to study testicular tissue and culverts where reason Abstract significant decrease

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Effect of leaves extract of Adhatoda vasicia plant against some wounds contamining bacteria by using sensitive test
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This study has been done on plant [Adhatoda vasicia , Acanthaceae family],which has been collected from gardens of university of Baghdad The leaves of plant were extracted by methanol alcohol obtain the crude extraction good ratio(30%).Eighty swabs or samples were collected from several wounds patients of hospitals in Baghdad city.These swabs were cultured on blood and MacConkey ager to isolate bacteria and identified by appearance and bio chemical tests.The results showed that(60)somples were positive(75%)for tests bacteria white the other(20)swabs were negative(25%).The bacteria were identified as Pseudomonas aeruginosa ,Staphylococcus awreus , Esherichia coli,Proteus spp and Klebsiella spp; and their number percentage were(32)isolates(

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