The study aimed to identify Trichoderma harzianum isolates morphologically and by using PCR teqnique and evaluate the antifungal activity of T. harzianum CA-07 crude extract against Trichophyton mentagrophytes and Microsporium canis from patients with dermatophytosis. T.harzianum isolates were collected from the soil of Baghdad University gardens and they were identified depending of morphological features on plate and microscopic examination. The genomic DNA of T.harzianum isolate was extracted at a final concentration of (400-600) μg / 2-3 g of wet mycelium and at a purity of 1.6-1.8and DNA sample was amplified with each of universal primers (ITS-1& ITS-4) to be used for detection of Trichoderma species. The crude extract was extracted from T.harzianum strain CA-07 by ethyl acetate with finalyield of 4.8gm., then different crude extract concentrations (0.5, 1, 2 and 4 mg/ml) were used against the clinical pathogenic fungi (T.mentagrophyte and M.canis) using agar well diffusion method. The results of identification of Trichoderma harzianum isolates by using PCR confirmed that the isolate was T.harzianum strain CA-07,and their crude extract exhibited significantly high antifungal activity against T. mentagrophyte and M. canis with high growth inhibition zones(14, 12mm) respectively at the lower concentration (0.5mg/ml)of crude extract.
This study was conducted to evaluate the efficiency of some chemicals and biological agents to induce systemic resistance (ISR) against to wheat common bunt disease caused by the two species of fungus Tilletia tritici (Bjerk.) Wint (T. caries (Dac.) Tul.) and T. laevis Kuhn (T. foetida (Wall.) Liro. Trails in the efforts to find an alternative, safe and environmentally friendly means to control the disease. Results of this study which carried out during two consecutive seasons for the years 2012 - 2013 and 2013 - 2014 at two different environmental locations. Seed treatment by (SA 100 and 200 mg/L, 500 ?–aminobutyric acid (BABA) and 1000 mg/L, Effective Microorganisms (EM1) 40 and 150 ml/kg seeds) have led to high significant redu
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Due to its various resistance mechanisms, Pseudomonas aeruginosa is the most prevalent opportunistic infection that kills hospitalized patients. Thus, therapeutic options become limited. Objective: The study aimed to estimate the antibiofilm effectiveness of Conocarpus erectus leaf extracts against MDR P. aeruginosa isolates and examines pelA and algD gene expression. Subjects and Methods: One hundred-fifty clinical samples were collected from five Baghdad hospitals between September 2021 and January 2022. Samples were grown on different mediums. Despite cetrimide agar's ability to detect P. aeruginosa, only 83 isolates developed at 42°C. VITEK 2 compact system identification followed. This study examined 83 of P. aeruginosa isolates for r
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Mercury can have significant impact on petroleum and related industries, it is also known to poison catalysts used in refining processes.Wet ash methods was widely used in determination of mercury in crude oil but the elemental and organic mercury are volatile and losses are also expected .An investigation of the use of Aqueous solution to prevent loss of mercury during wet digestion resulted in consistently good recoveries from crude oil samples.In this research diluted aqueous solution of sodium polysulfide is used and the parameters studied are polysulfide aqueous solution concentration, time, and ratio of the aqueous solution to crude oil,and will take different forms of heavy crude oil from several fields and the previous measuremen
... Show MoreAgaricus bisporus is cultivated edible mushroom . This study is made to evaluate the activity of A. bisporus extract on lipid profile in mice treated with peroxide. 40 females albino mice were chosen and divided into 4 groups (ten mice per each group). The first group was treated with normal saline as a control, the second group received 2ml/kg body weight of peroxide , While the third set received 2ml/kg body weight of peroxide plus 25 mg/ml A bisporus .Besides, the last fourth group received 25 mg/ml of A. bisporus. The Results have showed a significant increase (p≤ 0.05) in cholesterol(CH) in group 2 (250.01± 1.05)and group3 (204.25±1.80), while there wa
... Show MoreCarbamazepine (CBZ) is a narrow therapeutic index drug used in the treatment of trigeminal neuralgia and psychiatric disorders. Valerian (VAL) is a popular herbal product which should be prescribed to treat insomnia and anxiety. The study was designed to investigate the presence of significant pharmacokinetic (PK) interaction between Valerian (VAL) at different concentrations on Carbamazepine (CBZ) pharmacokinetic parameters in healthy male rabbits. In an in vivo, parallel-randomized controlled trial, the rabbits in three groups "first (control), second and third" were given oral doses of CBZ (50 mg/kg), for "second and third" groups (as test groups) rabbits were given (20 and 40 mg/kg/day) of the VAL respectively, as suspensi
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Background: Considering the antioxidant, anti-inflammatory, and antimicrobial properties of green tea, this study aimed to evaluate the histopathological effect of the sulcular irrigation of green tea extract in the treatment of experimental gingivitis in rabbit.
Materials and methods: For this experimental study, 45 male rabbits, separated in two groups, control non- irrigated group (5rabbits) and study group (40 rabbits), gingivitis induced by ligatures was packed subgingivally in the lower right central incisors of the experimental group for seven days. Then, the animals were randomly designated to two irrigated groups (20 rabbits
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؛ ١٨his study male and female albino mice werdministr^d doses of alkaloid and phenolic extracts of Allium cepa at doses of( 25 ,50,100, 200) mg / kg of( body weight). males and females were divided into four groups and each croup comprised mice were injected intra^ritonially daily for one week and orally ٢٠٢ one month . After which animals were killed and the serum was separated for biochemical analysis (total blood suger, total protein , otal cholesterol). Results showed significant decrease ( p< 0,05) in the total blood suger and total cholesterol on the serum of both males and females and significant increase( p< 0,05) in the total serum protein of both males and females of the two types of injection and oral administr
... Show MoreKlebsiella pneumoniae is one of common hospital-acquired bacteria causing nosocomial pneumonia, urinary tract infections, and intra-abdominal infections. The aim of this study is investigating the Modulation effect of Zinc Oxide nanoparticles (ZnO NPs) against multidrug resistant K. pneumonia which was isolated from Respiratory Tract Infections (RTIs). The results of isolation and characterization of bacterial specimens showed that 20.81% of RTIs were Klebsiella pneumoniae. The strongest isolate showed resistant for most usable antibiotics selected. Simultaneously, ZnO NPs were produced by an aqueous extract of Green Tea leaves as a reducing and stabilizer agent. The Ultraviolet-Visible (UV-Vis) spectrum was indicated a successful produc
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This research was carried out to evaluate the activity of crude juice of Olive on some cytogenetic parameters in mice like chromosomal aberration (CAs) and micronuclei formation(MN). The results showed that there was no significant difference between the crude juice (green and black)in CAs(3.77,4.10)and MN(0.25,0.25) in comparison with negative control (3.39,0.22)respectively. The interaction effect between the crude before and after treatment with mutagen MMC showed that the crude is one of the vital inhibitors of the mutagen by its ability in reducing the percentages of both the CAs and MN in bone marrow cells in mice.