The current study was conducted on 100 females who were divided into two main groups; 60 with breast cancer and 40 healthy controls. Blood samples were collected from both premenopausal and postmenopausal breast cancer and healthy women. The samples were appropriately processed for the analysis of trace elements (zinc, copper, and lead) by using flame atomic absorption spectrophotometry (FAAS). The results showed a highly significant decrease (p< 0.01) in the mean serum level of zinc of in both pre- and postmenopausal breast cancer women (71.7 + 5.1 and 70.4 + 5.4 µg/dL, respectively) compared with healthy controls (89.7 + 10.2 and 97.5 + 13.2 µg/dL,  respectively) . Also, a highly significant elevation (p< 0.01) in the mean serum level of copper (157.2 + 13.9 µg/dL and 157.4 +11.9 µg/dL, respectively) was found in pre- and  postmenopausal breast cancer women as compared to healthy controls (122.2 + 15.5 µg/dL and 112.2 + 15.8 µg/dL, respectively). Furthermore, a highly significant elevation (p< 0.01) in the mean blood level of lead (20.7 + 2.5 µg/dL and 19.9 + 1.7 µg/dL, respectively) was found in pre- and postmenopausal breast cancer women compared to healthy controls (15.1 + 2.0 µg/dL and 14.6 + 2.3 µg/dL, respectively). It is concluded that the disturbance in the homeostasis of some trace elements (zinc, copper, and lead) may lead to the development and even progression of breast cancer in both pre- and postmenopausal women.