Chlamydia pneumoniae is an intracellular gram-negative bacteria associated with lower and upper respiratory tract infections. Several studies, mostly achieved by serological assays, proposed a role for this bacteria in lung cancer risk. Therefore, this study aimed to evaluate the prevalence of Chlamydia pneuomoniae in fresh lung tissues of a sample of Iraqi patients with lung tumors, utilizing polymerase chain reaction (PCR) technique. . Chlamydia pneumoniae DNA was detected in 86.67% of samples. Besides, DNA sequencing of 16S rRNA gene revealed that our isolate is closely related to Chlamydia pneumoniae TW183 strain. It is concluded that Chlamydia pneumoniae is found in fresh lung tissues of patients, suggesting past infection, reinfection, or persistent form, which may play a role in the development or exacerbation of lung tumors. Our results confirm the high prevalence of this type of bacteria in Iraqi patients.
Background : the major focus of respiratory cytology is the diagnosis of lung cancer , carcinoma of the lung is now reported to be the most commonly diagnosed non- Cutaneous malignancy in the world. Iraq has faced the increase in incidence of this lethal type of cancer. Sputum cytology is a convenient method of screening and diagnosing primary epithelial tumor of the lung which is of many types include fresh smear ,Sacccomanno smear, and mailing container method.
Methods : Sputum cytological study was done on 50 patients suspected to have pulmonary carcinoma prepared by fresh smear method ,Saccomanno method ,and mailing container
method.One, two,or three samples taken from each patient.Slides were prepared
Background: High oncogenic-risk genotypes of human Papillomavirus (HPV) infect a wide range of human cells, including prostate tissue that give rise to benign prostatic hyperplasia and prostatic adenocarcinomas.
Objectives: This study aimed to detect DNA of HPV genotype-16 &18 using in situ hybridization technique in prostatic tissues from benign prostatic hyperplasia and prostatic adenocarcinomas, and elucidate the association between these HPV genotypes and prostatic carcinogenesis.
Patients and methods: Forty-eight (48) formalin-fixed, paraffin embedded prostatic tissue blocks were obtained ,among them (28) tissue biopsies from prostatic carcinoma with different grades and (20) benign prostate h
In this research, the concentration of radon gas has been calculated in the classrooms of schools in Diyala Governorate by using the nuclear track detector (CR-39), which was one of the organic solid-state nuclear detectors (SSNTDs). After calculating the radon gas concentration, the lung cancer cases were calculated. The results showed that the lowest value was found in (Alshumue) school (4.753) people. In contrast, the highest value was found in (Habhib) school (20.421). The average values of lung cancer cases in Diyala governorate were equal to (9.319) per person. The results showed that the number of lung cancer cases per year per million persons in Diyala Governorate schools was below the allowed limit from (ICRP) agency (170-230) p
... Show MoreLung cancer, similar to other cancer types, results from genetic changes. However, it is considered as more threatening due to the spread of the smoking habit, a major risk factor of the disease. Scientists have been collecting and analyzing the biological data for a long time, in attempts to find methods to predict cancer before it occurs. Analysis of these data requires the use of artificial intelligence algorithms and neural network approaches. In this paper, one of the deep neural networks was used, that is the enhancer Deep Belief Network (DBN), which is constructed from two Restricted Boltzmann Machines (RBM). The visible nodes for the first RBM are 13 nodes and 8 nodes in each hidden layer for the two RBMs. The enhancer DBN was tr
... Show MoreIn the present study, the effects of brake pad particles of lung and liver histological sections were evaluated for (60) adult male mice. The animals were divided into three groups ( A,B,C) according to the periods of exposure (4, 8, and 12) weeks respectively exposed to brake pad particles in addition to the control groups (F) exposed to fresh air only. A special inhalation chamber designed locally has been used to expose the animals. The exposure to brake pad particles was (2.228) µg/m³ for 30 min/day, 5 days/week for (4,8and12) weeks respectively.
The examination in group (A) of the histological sections of the lung showed the thickness of interalveolar septa. Also, a congestion of alveolar capillary was marked indicat
... Show MoreBackground: lung function is closely related to anthropometric variables. Anegative relationship between different adiposity markers and lung function has been well established.
Type-1 diabetes is defined as destruction of pancreatic beta cell, virus and bacteria are some environmental factor for this disease. The study included 25 patients with type-1 diabetes mellitus aged between 8 – 25 years from Baghdad hospital and 20 healthy persons as control group. Anti-rubella IgG and IgM, anti-Chlamydia pneumonia IgG and IgM were measured by ELISA technique while anti-CMV antibody were measured by immunofluorescence technique. The aim of current study was to know the trigger factor for type-1 diabetes. There were significant differences (P<0.05) between studied groups according to parameters and the results lead to suggest that Chlamydia pneumonia, CMV and rubella virus may trigger type-1 diabetes mellitus in Iraqi pat
... Show MoreAbstract
Background
Stathmin1 (also known as metablastin) is a major microtubule-depolymerizing protein that involved in cell cycle progression and cell motility.
Stathmin1 has been found to be up-regulated in some cancers and correlated with cell differentiation and proliferation.
Stathmin1 is a major cytosolic phosphoprotein that regulates microtubule dynamics and is associated with malignant phenotypes in various cancers, including non-small cell lung cancer.
Objective
To evaluate the role of Immunohistochemical expression of stathmin1 in non-small
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