Disodium phosphate (PubChem CID: 24203)
Methotrexate
Methotrexate (PubChem CID: 126941)
Potassium dihydrogen phosphate (PubChem CID: 516951)
Silibinin
Silibinin hemisuccinates (PubChem CID: 150708)
Spermatogenesis
Testicular tissue
Testosterone
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Methotrexate (MTX) is widely used chemotherapeutic agent with different side effects including germ cells toxicities. Silibinin is one of the structural isomers of silymarin, with different phytotherapeutic applications, and its possible protective effects against MTX induced germ cells damage were investigated in this work. Twenty five male mice were divided into five groups (n=5) allocated as follows: Group 1 received buffer for five days given by single intraperitoneal (IP) injection per day; Group 2 in addition to buffer for five days, animals received at day five single dose of 20mg/kg of MTX IP. Groups (3, 4, and 5) received respectively, (50, 100, or 150mg/kg body weight) of silibinin IP single daily dose for five days then at day fi
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