This study was conducted to investigate the antibacterial activity of green synthesized copper oxide nanoparticles (CuO NPs) using Aloe vera. Initially, bacteria were collected from clinical samples of patients having otitis media infection and the isolates were identified at the species level following biochemical tests. Copper oxide nanoparticles were prepared by green synthesis using Aloe vera leaves and characterized using UV- visible spectroscopy at 260 nm peak. The shape and size were determined by using transmission electron microscopy (TEM) and the dimensions of the particles were more precisely determined by using scanning electron microscopy (SEM) and x-ray diffraction (XRD). Different concentrations of nanoparticles (25-50-75-100 µg/ml) were tested for antibacterial activity by using the well diffusion method. The results showed that the shape of CuO NPs was spherical with a size range of 40-10 0nm. The TEM images revealed average of dimensions of 32.34, 35.63, 51.85, 74.71 and 100 nm. The antibacterial activity results of the nanoparticles showed the following growth zone inhibition values for the different bacterial species used: Staphylococci aureus 17.1 mm, Pseudomonas aeruginosa 17 mm, Escherichia coli 16.8mm, Staphylococci epidermidis 16.4mm, Pseudomonas oryzihabitans 15.3mm, Klebsiella pneumonia 13.5mm, Citrobacter freundii 12.7mm, Enterobacter Cloacae 12.2 mm, Proteus vulgaris 8mm, Concerning the virulence factor production, the nanoparticle inhibited the production of biofilm and urease more than other virulence factors, such as gelatinase, hemolysin, protease and lecithinase, by some Gram negative and positive bacterial isolates.
Antibacterial Activity of Bioactive Glass 45S5 and Chitosan Incorporated as Fillers into Gutta Percha, Ahmed I AL-Jobory*, Raghad AL-Hashimi
A novel series of chitosan derivatives were synthesized via reaction of chitosan with carbonyl compounds and grafted it’s by with different amine compounds substituted hydrogen. The produced polymers were characterized by different analyses FTIR, 1HCNMR, XRD, DSC and TGA. Solubility in water as well as many solvent was investigated, antibacterial activity of chitosan and its derivatives against two types of bacteria E. coli and S. aureus was also investigated. The results showed that derivatives sort of have antibacterial activities against Esherichia coli (Gram negative) better than chitosan whilst compound IX has better antibacterial against Staphylococcus aureus (Gram positive). SEM analysis showed that increase of surface roughness wi
... Show MoreBiomedical alloy 316L stainless steel enhancing to replace biological tissue or to help stabilize a biological structure, such as bone tissue, enhancing were coated with deposition a thin layer of silver nanoparticles as anti-bacterial materials by using DC- magnetron sputtering device. The morphology surface of The growth nanostructure under the influence of different working pressure were studied by atomic force microscope. The average grain size decrease but roughness of the silver thin layer was increased with‖ ―increasing the working pressure. The thickness of silver thin layer was increased from 107 nm at 0.08 mbar to 126 nm at 1.1 mbar. Antimicrobial activity of silver thin layers at different working pressure were studied. Th
... Show MoreLactobacillus is one of Lactic Acid Bacteria group, they are known to exhibit antagonistic activity against pathogenic organisms. This study evaluates the protective effect of Lactobacillus acidophilus Crud Bacteriocin like substance against growth of some food borne pathogenic bacteria. Antimicrobial effect of crud bacteriocin like substance was tested against the growth of S. typhi and S. aureus that isolated from food samples by agar well diffusion assay.
The results showed that the crud extract of bacteriocin like substance had antibacterial activity against the two tested bacteria, and the effect against S. typhi was greater than that of S. aureus , the zone of inhibition was (15)mm against S.&n
Condensation of 4-methoxybenzoyl hydrazine with 4- aminobenzoic acid in the presence of POCl3 gave the oxadiazole derivative [III] .This compound was demethylated with aluminium chloride to give series of 2- (4-hydroxy phenyl)-5-(4-amino phenyl)
1,3,4-oxadiazole [IV]. Series of Schiff s bases [V]n were synthesized by the condensation of compound [IV] with 4-n-alkoxy benzaldehyde in the presence of glacial acetic acid. Condensation of compounds [VI]n. with adipoyl chloride in dry pyridine leads to the formation of a new homologous series [VI]n. The structures of the synthesized compounds were confirmed by physical and spectral means The new compounds [VI]n have been screened for their antibacterial activities . The results
A novel series of chitosan derivatives were synthesized via reaction of chitosan with carbonyl compounds and grafted it’s by with different amine compounds substituted hydrogen. The produced polymers were characterized by different analyses FTIR, 1HCNMR, XRD, DSC and TGA. Solubility in water as well as many solvent was investigated, antibacterial activity of chitosan and its derivatives against two types of bacteria E. coli and S. aureus was also investigated. The results showed that derivatives sort of have antibacterial activities against Esherichia coli (Gram negative) better than chitosan whilst compound IX has better antibacterial against Staphylococcus aureus (Gram positive). SEM analysis showed that increase of surface roughness wi
... Show MorePolyacetal was synthesized from the reaction of Polyethylene glycol with4- dimethylaminobenzaldehyde.Polymer metal complex was synthesized by the reaction with Ag+; polymer blend with polyvinyl alcohol was synthesized solution casting technique. All synthesized compounds were characterized by FT-IR in addition to the antimicrobial activity. The FT-IR spectra indicate the formation of the polyacetal. The DSC resultsindicatethe thermal stability regarding the synthesized polymer blends. The synthesized polyacetal, its metal complex and PA blend against four types of bacteria (gram+ve) Staphylococcus aureas, Bacillus subtilis and (gram –ve)Klebsiella pneumoniae, Escherichia Coli w
... Show MoreIn this study, the zinc oxide NPs have been synthesized from the fresh pomegranate peels extract using the precipitation method. The ZnO nanoparticles were produced from the reaction of fresh peels extract with zinc acetate salt which was used as zinc source in the presence of 2 M NaOH. The green synthesized nanoparticles were characterized through X-ray diffraction (XRD), UV-Vis diffuse reflection spectroscopy, Fourier transform infrared spectroscopy (FTIR), and Atomic force microscopy (AFM). The XRD patterns confirm the formation of hexagonal wurtzite phase structure for ZnO synthesized using pomegranate peels extract with average crystalline size of 28 nm. FTIR spectra identify the presence of many active functional groups for the pom
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