The spread of antibiotic resistant bacteria is a worldwide problem. Due to the importance of P. aeruginosa as a multidrug resistant bacterium, this study aimed, through molecular techniques, to detect point mutations in chromosomal genes responsible for the quinolones class of antibiotics resistance. A total of 52 isolates from burn infections were identified using specific primers for P. aeruginosa 16S rDNA. Ciprofloxacin minimum inhibitory concentrations (MIC) were estimated using the agar dilution assay. DNA sequences of the quinolone resistance-determining regions of gyrA and parC were determined for detecting the mutations found in these genes and the relations among the isolates by constructing phylogenetic trees. The results revealed that only 43 (82.7%) of isolates were P. aeruginosa, of which 31 (72.06%) were resistant to different concentrations of ciprofloxacin, ranging between 4 and >32 µg/ml. Twenty six isolates were selected for sequencing, including sensitive, intermediately resistant, and highly resistant to ciprofloxacin. The ciprofloxacin sensitive isolates did not exert any amino acid alterations in gyrA or parC genes; however, a single intermediately resistant isolate had a single mutation at each gene. Of the total resistant isolates (20), 6 isolates had no mutations at different MIC levels, While 14 isolates had Thr-83-Ile substitution in gyrA and Ser-87-Leu substitution in parC, only five isolates had a second mutation, namely Asp-87-Asn, in gyrA. The phylogenetic analysis of the studied groups showed divergence from the P. aeruginosa PAO1 and PAO1OR reference strains due to increased mutations and polymorphisms in studied isolates. In conclusion, P. aeruginosa occurrence was increased in burn infections and the fluoroquinolones in current use are not as effective as before; the main resistance mechanism in local clinical isolates of P. aeruginosa is mutations, where the main target of fluoroquinolones is gyrA gene.
This paper examines the finding of spacewise dependent heat source function in pseudoparabolic equation with initial and homogeneous Dirichlet boundary conditions, as well as the final time value / integral specification as additional conditions that ensure the uniqueness solvability of the inverse problem. However, the problem remains ill-posed because tiny perturbations in input data cause huge errors in outputs. Thus, we employ Tikhonov’s regularization method to restore this instability. In order to choose the best regularization parameter, we employ L-curve method. On the other hand, the direct (forward) problem is solved by a finite difference scheme while the inverse one is reformulated as an optimization problem. The
... Show MoreA liquid membrane process of Alkaloids extraction from Datura Innoxia solution was studied applying pertraction process in rotating discs contactor (RDC). Decane as a liquid membrane and dilute sulphuric acid as stripping solution were used. The effect of the fundamental parameters influencing the transport process, eg type of solvent used, effect of disks speed, amount of liquid membrane and effect of pH for feed and strip solution. The transport of alkaloids was analysed on the basis of kinetic laws of two consecutive irreversible first order reactions. Thus, the kinetic parameters (k1, k1,, tmax, and) for the transport of alkaloids were determined. The effect of organic membrane type on percentage of Alkaloids transport was found to be i
... Show MoreThis study aimed to reveal the stage for teachers of basic training needs from the perspective of workers in Mafraq Governorate, through a survey of a sample of counselors look at the Ajloun area schools reached (58) counselors.
To achieve the objective of the study was constructed questionnaire where they are finalized (18) items distributed on two dimensions (professional needs, performance requirements) and after confirmation of the validity and reliability have been applied to the sample where the results showed that training needs were high, both on a professional or per formative level .
The results also showed no statistically significant differences in the areas of tool due to gender, educational qualification. The study co
sanaa tareq, Baghdad Science Journal, - Cited by 1
Background: Noise injury and hearing loss are significant problems in Nigerian community. The hearing loss sustained from noise injury can have disabled personnel and social consequences for the affected persons and their families. This study was carried out to quantify the noise level of over eighty (80) different sources using the sound level meter (GB: 2266204) at different locations in Akure, capital of Ondo State, Nigeria. Standard procedure specified by the manufacturer was strictly followed and the results were statistically analyzed. The range of results obtained were: household products (48.5±0.5 - 105.7±3.2dBA), traffic (60.9±0.6 - 77.5±1.2dBA), market (55.0±0.5 - 68.1±0.7dBA), automobile/ workshop (65.7±0.5 - 105.7
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Prepositions have a key function, which is to associate parts of a sentence, called letters In addition, it adds the meanings of the verbs to the names, ie reached by them, and called by the coffin characters Qualities..
I chose to search for the meaning of the preposition "from" in Surat Maryam to know its meanings in terms of originality and expansion, so the title of the research (the meanings of preposition "from" in terms of originality and expansion in Surat Maryam model(
The study area is part of the city of Samawa in Al Muthanna Governorate in southern Iraq. The study area is located to the west of Samawa city bounded by the north latitudes 31⁰11'-31o42' and east longitudes 44o58'- 45⁰16'and its groundwater resources are developed for supply and irrigation purposes. In order to evaluate the quality of groundwater in the study area, twenty three groundwater samples were collected and analyzed for physical and chemical parameters. Hydrochemical analysis showed that the groundwater of the study area is excessively mineralized and very hard. The increase in flow length of groundwater in the study area caused a change in water
... Show MoreThe coma gasses consist of molecules liberated from the nucleus by solar heating and relative sublimation. Once they have left the nucleus, these molecules in the coma are exposed to direct solar radiation and can be damaged in various ways due to the combined action of these reactions.
One of some complex problems facing the research in this field is that the Maxwell-Boltzmann equation gives distribution function for one kind of particles which have same masses, but the gas has multi-groups of particles (Carbon, Neon, Sodium … etc.), where all these components must be in one program to extract average velocity of all and calculate particles velocity to each band. This problem is solved here by Matlab program a
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