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Role of oqxA and oqxB Genes in the Development of Multidrug Resistant Phenotype among Clinical Klebsiella pneumoniae Isolates from Various Cases
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This study investigated the prevalence of oqxA and oqxB genes and their effective roles in the development of multidrug resistant (MDR) phenotype among clinical isolates of Klebsiella pneumoniae. Out of 150 clinical samples, 50 (33%) isolates were recognized as K. pneumoniae according to the morphological and biochemical properties. The minimum inhibitory concentrations (MICs) assay revealed that the resistance values of the isolates were 43 (86%) against ceftriaxone (4- ≥64 µg/ml), 42 (84%) against ceftazidime (16- ≥64 µg/ml), 41 (82%) against cefepime (≥16 µg/ml), 21 (42%) against ertapenem (≥8 µg/ml), 18 (36%) against imipenem (4- ≥16 µg/ml), 15 (30%) against ciprofloxacin (≥4 µg/ml), 11 (22%) against levofloxacin (≥8 µg/ml), 45 (90%) against nitrofurantoin (128- ≥512 µg/ml), 36 (72%) against trimethoprime-sulfamethoxazole (≥320 µg/ml), and 4 (8%) against tigecycline (≥8 µg/ml). Genotype detection revealed that oqxA was found in 48 (96%) of K. pneumoniae isolates, whereas oqxB was found in 6 (12%) isolates. The MDR phenotype was observed in 40 (80%) isolates, of which 38 (95%) were harbored oqxA and/or oqxB genes. DNA sequencing of oqxA revealed the presence of three silent mutations. The phylogenetic tree of oqxA variants showed a significant deviation of these variants from K. pneumoniae species. The high prevalence of oqxA among K. pneumoniae isolates may contribute to the reduction of their susceptibility to multiple antimicrobial agents.

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Publication Date
Mon Jul 02 2007
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Spontaneous Abortion and Failure of Human Cytotrophoblasts to adopt a vascular adhesion phenotype
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Background: Differentiating cytotrophoblasts transform their adhesion receptor phonotype so as to resemble the endothelial cells they replace a process that is required for normal placentation, and any defect in this adhesion phenotype switch might lead to pregnancy failure.
Patients and Methods: Curate samples of the materno-fetal interface were taken from 20 women with spontaneous first trimester abortion and 6 control women undergone elective termination of pregnancy in the first trimester. Immunohistochemistry analysis of paraffin embedded sections of these curate samples was performed using monoclonal antibodies for cytokeratin, PECAM-1 and VCAM-1.
Results: Cytokeratin showed positive immunostaing

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Publication Date
Sun Oct 22 2023
Journal Name
Iraqi Journal Of Science
Biofuel Production from Cellulosic Wastes by Local Isolates of Streptomyces sp.
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The current study aims to produce cellulase enzyme from Streptomyces spp. isolates and study the effect of some cultural conditions on cellulase production; biofuel production from cellulotic waste through enzymatic and acids hydrolysis. Out of 74 isolates of Streptomyces sp. were screened for cellulse production in solid and liquid media. Results showed higher capability of isolate Streptomyces sp. B 167 for cellulase production and bioconversion of cellulose, therefore selected for further studies. The results of optimization revealed that the cellulase enzyme productivity by the selected isolate reached 2.1 and 2.28 U/ml after 48 h of incubation time and pH 7 respectively. Cellulase productions in tested isolate improved (2.57 U/ml) b

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Publication Date
Sun Jul 02 2023
Journal Name
Iraqi Journal Of Science
Biofuel Production from Cellulosic Wastes by Local Isolates of Streptomyces sp.
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The current study aims to produce cellulase enzyme from Streptomyces spp. isolates and study the effect of some cultural conditions on cellulase production; biofuel production from cellulotic waste through enzymatic and acids hydrolysis. Out of 74 isolates of Streptomyces sp. were screened for cellulse production in solid and liquid media. Results showed higher capability of isolate Streptomyces sp. B 167 for cellulase production and bioconversion of cellulose, therefore selected for further studies. The results of optimization revealed that the cellulase enzyme productivity by the selected isolate reached 2.1 and 2.28 U/ml after 48 h of incubation time and pH 7 respectively. Cellulase productions in tested isolate improved (2.57 U/ml) b

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Publication Date
Sat Apr 15 2023
Journal Name
Iraqi Journal Of Science
Detection of Adenovirus antigen and its correlation with clinical signs among children with gastroenteritis
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A total of 90 stool sample was collected from patients with gastroenteritis to
detect adenovirus antigen among diarrhea cases. They were tested by general stool
examination (GSE), rapid immunochromatographic test and Enzyme Linked
Immunosorbent Assay (ELISA). GSE showed that adenovirus gastroenteritis
infection resulted in non-bloody diarrhea, the existence of RBCs in 7% and Pus in
37% of the samples, Entamoeba histolytica trophozoite and cyst were seen in 3%
and 2% of the samples respectively. The rapid test showed that 21% of samples
were positive for rotavirus, 8% for adenovirus and 3% for astrovirus. Meanwhile,
the ELISA test showed that adenovirus was positive in 9% of the samples. These
findings establish

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Publication Date
Mon Jan 01 2018
Journal Name
Jordan Journal Of Biological Sciences
Expression of biotransformation and antioxidant genes in the liver of albino mice after exposure to aflatoxin B1 and an antioxidant sourced from turmeric (Curcuma longa)
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The present investigation aims to determine the effects of aflatoxin B1 (AFB1) on biotransformation and antioxidant genes and the protective effects of curcumin, present in turmeric (Curcuma longa) powder (TMP). Specifically, the study included four groups of albino mice were fed for 30 days on diet Group I: Control, Group II: animals fed on the conventional basal diet supplemented with 0.5% food grade TMP that supplied 74 mg/kg total curcuminoids. Group III contained animals reared on conventional basal diet supplemented with 1.0 ppm AFB1 supplied by ground aflatoxin culture material (760 ppm AFB1). Finally, Group IV comprised of albino mice fed with basal diet supplemented with 1.0 ppm AFB1 and 0.5% TMP that supplied 74 mg/kg of the

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Publication Date
Sat Jul 01 2023
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Prevalence and clinical characteristics of depression among elderly patients attending primary health care centers in Diyala Governorate
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Background: In the elderly, depression is the most frequent mood condition. It has been linked to major consequences such as disability, functional decline, decreased quality of life, higher mortality, it is the most costly health problem among elderly, a little study is written about depression among elderly patients in Iraq.

Objectives: To determine the prevalence of depression in elderly aged 60 years and more who visited primary health care centers in Diyala.

Methodology: It is a descriptive, cross-sectional study that was conducted on 218 patients from PHCs of 2 sectors: Al-Kales and 1st Baquba, Diyala governorate-Iraq. A direct interview was conducted by

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Publication Date
Sun Sep 01 2019
Journal Name
مجلة بحوث
The role of university in promoting the culture of volunteering among students (Mustansiriya University Model)
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Abstract The aim of this study is to identify the role played by the university in reinforcing the culture of voluntary work in college students and sequencing these roles according to their priority. To achieve this aim, the researcher used the descriptive approach. After being informed of the literature background and of the previous studies related to the core aim of this recent study, the researcher has built up a questionnaire of (20) items investigating the role of university in reinforcing the culture of voluntary work in the college students. The standardized features of the questionnaire have been checked for the purpose of the questionnaire validity (virtual and constructive validity) and stability (reconstruction and Cronbach's A

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Publication Date
Tue Feb 26 2019
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
Role of the General Dudget in the Achievement of Sustainable Development: دراسة تحليلية مقارنة للموازنة العامة العراقية
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The traditional method adopted in the preparation of the general budget in Iraq is not consistent with developments in the size specification response and spending and the associated weakness in the size of the amounts earmarked for investment projects which could adversely affect future generations and not to enable them to continue the development, which requires talking to estimate the adoption of style public expenditure in the state budget and reduce waste and extravagant where and invest public revenues of the state in investment projects and preservation of the environment and natural resources in order to ensure the benefit of future generations system, according to the system serves to achieve the overwhelming majority of member

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Publication Date
Sun Jul 04 2010
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The role of pepsinogen test among the patients with gastric cancer.
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Background: Identification the relationship of pepsinogen test (pepsinogenI and I /pepsinogen II ratio) and gastric cancer patients make the gastric cancer early diagnosed and prolong the life surviving rate.
Patients and Methods: Serum pepsinogen I and pepsinogen II (PG I and PG I / PG II ratio) measured in 50 patients with gastric cancer ,30 gastritis patients diagnosed , and 16 healthy control by using enzyme immunoassay in those study groups, and the results of PG I and PG I / PG II ratio were comparing to 50 gastric cancer patients whom diagnosed histopathologically according the stomach regions, while the gastritis patients diagnosed endoscopically.
Results: This study showed that the age of pati

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Publication Date
Sat Dec 30 2023
Journal Name
Iraqi Journal Of Science
Detection of Resistance Genes (Blandm and Blavim) of Escherichia Coli in the Aquatic Environment
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The study aims to detect the presence of carbapenems genes and the prevalence of antibiotic-resistant E. coli in the Tigris River. Samples were collected from three sites of the Tigris River: S1Adhamiya, S2 Medical city hospital, and S3 Abu Nuwas. It diagnosed 40 isolates of E. coli out of 67 isolates of bacteria by Vitek2. The antibiotic sensitivity was determined by the disk diffusion method. E.coli isolates were tested against 7 antibiotics these belonged to β-lactam, Carbapenem. Also, the resistance genes) blaVIM and blaNDM) detected for these isolates of E. coli.  The results appeared resistance of  E.coli against AMC 82.5%, PRL 62.5%, AM 55%, and moderate resistance

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