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Detection of Listeria monocytogenes in Several Types of Frozen Meat in Baghdad city
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Detection of pathogenic bacteria, such as Listeria  monocytogenes, in food is crucial for safeguarding public health in Iraq.  Forty five samples of frozen meat (15 samples of each of minced red meat, chicken, and fish) were collected from different markets in Baghdad city. Molecular (RT-PCR) and culturing (conventional microbiological examination) methods were used to determine the level of contamination of L. monocytogenes in these types of meat.

For the culturing method, TSYEB broth was used as an enrichment medium, whereas BALCAM medium (HiMedia) with the listeria selective supplement FD061 was used as a selective medium, for the isolation and identification of this bacterium. The isolates were confirmed microscopically and biochemically. The results of the culturing method showed that the total number of the isolates of L. monocytogenes was 14/45 (31.1%). The incidence of this bacterium was high in fish (11/15, 73.3%), while it was low in the other two types of meat.  2/15 (13.3%) in red meat and 1/15 (6.7 %) in chicken.

Molecular detection of each sample of the bacteria was performed using RT-PCR technique after preparing the Genomic DNA extraction of these samples according to the protocol provided by ReliaPrep™ Blood gDNA Miniprep System kit (Promega, USA). The PCR primers and the hybridization probe ((Macrogen, Korea) were used to target the inlA gene sequence (specific for L. monocytogenes). The results of the RT-PCR assay showed that 10/45 (22.2%) of the samples were positive for L. monocytogenes, which was detected only in fish samples ((10/15, 66.7%), while not found in minced red meat and chicken. However, our results showed differences when compared to other previous works because there were many studies found that the highest contamination rate was in red meat products.

We conclude that the PCR kit used for the detection of L monocytogenes appears to give accurate results in the diagnoses of this bacterium in meat products and in comparison with the other routine diagnosis methods in the laboratory, which included culturing and doing biochemical tests which last for approximately 7 days, the RT-PCR technique was able to confirm the findings within 48 hours.

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Publication Date
Fri Oct 24 2014
Journal Name
Journal Of Chemical And Pharmaceutical Research
Synthesis, characterization and antimicrobial activities of [Fe(II), Co(II), Ni(II),Cu(II) and Zn(II)] mixed ligand complexes schiff base derived from amoxicillin drug and 4-(dimethylamino)benzaldehyde with nicotinamide
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New Schiff base ligand (E)-6-(2-(4-(dimethylamino)benzylideneamino)-2-(4-hydroxyphenyl)acetamido)-3,3- dimethyl-7-oxo-4-thia-1- azabicyclo[3.2.0]heptane-2-carboxylic acid = (HL) was synthesized via condensation of Amoxicillin and 4(dimethylamino)benzaldehyde in methanol. Figure -1 Polydentate mixed ligand complexes were obtained from 1:1:2 molar ratio reactions with metal ions and HL, 2NA on reaction with MCl2 .nH2O salt yields complexes corresponding to the formulas [M(L)(NA)2Cl],where M=Fe(II),Co(II),Ni(II),Cu(II),and Zn(II), A=nicotinamide .

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Publication Date
Wed Jul 01 2020
Journal Name
International Journal Of Pharmaceutical Research
Synthesis and identification of some new N-substituted quinazoline-4-one, thiazine-4-one and tetrazoline rings incorporating N-ethyl-2-(benzylthio)benzimidazole acetate and study their application as anti-oxidant agent
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Publication Date
Sat Sep 23 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Synthesis and Characterization of New ligand ' ((2-{1..,[(2-hyd_roxy;..benzyli_dene)-hydrazono]- ethyl} benzen_e-l, 3, s--moll and ItComplexes W·itll. (Mn.(11 Fe(n), Cd<11J, a-nd Hg(n).) Tons
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Publication Date
Sat Jun 03 2023
Journal Name
Iraqi Journal Of Science
Effect Effect Effect Effect Effect Effect Effect of Thickness on Some Physical PropertiesThickness on Some Physical PropertiesThickness on Some Physical PropertiesThickness on Some Physical PropertiesThickness on Some Physical Properties Thickness on Some
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The effect of thickness variation on some physical properties of hematite α-Fe2O3 thin films was investigated. An Fe2O3 bulk in the form of pellet was prepared by cold pressing of Fe2O3 powder with subsequent sintering at 800 . Thin films with various thicknesses were obtained on glass substrates by pulsed laser deposition technique. The films properties were characterized by XRD, and FT-IR. The deposited iron oxide thin films showed a single hematite phase with polycrystalline rhombohedral crystal structure .The thickness of films were estimated by using spectrometer to be (185-232) nm. Using Debye Scherrerś formula, the average grain size for the samples was found to be (18-32) nm. Atomic force microscopy indicated that the films had

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Publication Date
Thu Jan 01 2015
Journal Name
Al–bahith Al–a'alami
Health Awareness Throush Communication Media
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Means of communication has a great impact on all fields of awareness including health awareness by increasing the knowledge of community about health and developing their abilities to improve human health and cultural awareness. According to the importance of health awareness for a community to develop their intellectual and physical integrity, the researcher has found that it is essential to know the role of means of communication as a source of information for many students being active and main segments to build their society intellectually, socially and economically.

The research has focused on the study of health awareness among students and their health knowledge derived from the means of communicat

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Publication Date
Thu Apr 20 2023
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
A Proposed Wavelet and Forecasting Wind Speed with Application
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Time series analysis is the statistical approach used to analyze a series of data. Time series is the most popular statistical method for forecasting, which is widely used in several statistical and economic applications. The wavelet transform is a powerful mathematical technique that converts an analyzed signal into a time-frequency representation. The wavelet transform method provides signal information in both the time domain and frequency domain. The aims of this study are to propose a wavelet function by derivation of a quotient from two different Fibonacci coefficient polynomials, as well as a comparison between ARIMA and wavelet-ARIMA. The time series data for daily wind speed is used for this study. From the obtained results, the

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Publication Date
Thu Jan 14 2021
Journal Name
Iraqi Journal Of Science
Fuzzy PID Gain Scheduling Controller for Networked Control System
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The use of a communication network in the closed loop control systems has many advantages such as remotely controlling equipment, low cost, easy to maintenance, efficient information transmission, etc. However, the Networked Control System (NCS) has many drawbacks, such as network-induce end-to-end time delay and packet loss, which lead to significant degradation in controller performance and may result in instability. Aiming at solving performance degradation in NCS, this paper propose to take the advantages and strength of the conventional Proportional-Integral-Derivative (PID), Fuzzy Logic (FL), and Gain Scheduling (GS) fundamentals to design a Fuzzy-PID like-Gain Scheduling (F-PID-GS) control technique, which has been proved to be ef

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Publication Date
Tue Apr 20 2021
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Bayesian Structural Time Series for Forecasting Oil Prices
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There are many methods of forecasting, and these methods take data only, analyze it, make a prediction by analyzing, neglect the prior information side and do not considering the fluctuations that occur overtime. The best way to forecast oil prices that takes the fluctuations that occur overtime and is updated by entering prior information is the Bayesian structural time series (BSTS) method. Oil prices fluctuations have an important role in economic so predictions of future oil prices that are crucial for many countries whose economies depend mainly on oil, such as Iraq. Oil prices directly affect the health of the economy. Thus, it is necessary to forecast future oil price with models adapted for emerging events. In this article, we st

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Publication Date
Mon Aug 01 2022
Journal Name
Baghdad Science Journal
Optimized Artificial Neural network models to time series
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        Artificial Neural networks (ANN) are powerful and effective tools in time-series applications. The first aim of this paper is to diagnose better and more efficient ANN models (Back Propagation, Radial Basis Function Neural networks (RBF), and Recurrent neural networks) in solving the linear and nonlinear time-series behavior. The second aim is dealing with finding accurate estimators as the convergence sometimes is stack in the local minima. It is one of the problems that can bias the test of the robustness of the ANN in time series forecasting. To determine the best or the optimal ANN models, forecast Skill (SS) employed to measure the efficiency of the performance of ANN models. The mean square error and

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Publication Date
Tue Jan 01 2019
Journal Name
Aip Conference Proceedings
Temperature dependence energy distribution function for proton-tritium fusion reaction
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The physical behavior for the energy distribution function (EDF) of the reactant particles depending upon the gases (fuel) temperature are completely described by a physical model covering the global formulas controlling the EDF profile. Results about the energy distribution for the reactant system indicate a standard EDF, in which it’s arrive a steady state form shape and intern lead to fix the optimum selected temperature.

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