The aim of this study is the determination of the anti-adhesion activity of active compounds extracted from plants in the inhibition of adhesion ability of C. albicans that is used as a virulence factor for infection. Adherence to host surfaces is a primary factor in the colonization of human tissues by fungi, which can also adhere to the surfaces of medical devices and form biofilms. Medicinal plants possess therapeutic properties or beneficial pharmacological effects on the body. A total of one hundred samples were collected from female patients with vaginal infection in two hospitals in Baghdad city for three months. The fungi were isolated and identified by microscopic morphology, morphological features on culture media, and Vital Index of Traditional Environmental Knowledge (VITEK 2) System. Extraction and purification of plant active compounds, vasicine from Adhatoda vasica and gallic acid from Punica granatum L. , were performed. The active compounds were used as anti-adhesion factors and the minimum inhibition concentration (MIC) against Candida albicans was determined. We also used the antifungal drug fluconazole for comparison as a control. The results of vaginal discharge characterization of clinical samples showed that 30% of the infections are caused by fungi (100% were Candida albicans), 47% by Gram negative bacteria, and 23% by Gram positive bacteria. The results of MIC assays showed that the plant extract and the antifungal drug do not seem to have fungicidal effects on the isolates of C. albicans tested. The ability of C. albicans isolates for adhesion to the epithelial cells was detected by the calculation of the number of adherent C. albicans cells under light microscope. The activity of vasicin and gallic acid appeared to be approximately equal on the number of C. albicans adherent on epithelial cells. Vasicin concentration activity used in mg/ ml was higher than that used in µg/ ml, with less numbers of C. albicans adhered on epithelial cell. However, treatment with fluconazole showed that more than half of the number of C. albicans were non-adherent on epithelial cells when added to the suspension, in contrast to the control (C. albicans without the extracts or antifungal drug).
Corrosion of steel reinforcement is one of the biggest problems facing all countries in the world like bridges in the beach area and marine constructions which lead to study these problems and apply some economical solutions. According to the high cost of repair for these constructions, were studied the effect of using kind of chemical compounds sodium nitrite(NaNO2) and sodium silicate(Na2SiO3) as corrosion inhibitors admixture for steel bars that immersed partially in electrolyte solution (water + sodium chloride in 3% conc.) (Approximately similar to the concentration of salt in sea water). The two inhibitors above added each one to the electrolyte solution at concentrations (0.5%, 1% and 2%) for both
... Show MoreIn this paper, an Anti-Disturbance Compensator is suggested for the stabilization of a 6-DoF quadrotor Unmanned Aerial vehicle (UAV) system, namely, the Improved Active Disturbance Rejection Control (IADRC). The proposed Control Scheme rejects the disturbances subjected to this system and eliminates the effect of the uncertainties that the quadrotor system exhibits. The complete nonlinear mathematical model of the 6-DoF quadrotor UAV system has been used to design the four ADRCs units for the attitude and altitude stabilization. Stability analysis has been demonstrated for the Linear Extended State Observer (LESO) of each IADRC unit and the overall closed-loop system using Hurwitz stability criterion. A minimization to a
... Show MoreIn the present study, the effect of vasicine alkaloid separated from Adhatoda vasica as an inhibitor agent on the activity of proteases enzyme isolated from Pseudomonas aeruginosa was investigated. forty isolates of Pseudomonas aeruginosa were collected from local hospital in Baghdad and then their ability for producing proteases was screened using quantification and semi- quantitative methods. Pseudomonas aeruginosa P1 was selected as the highest protease producer, which next identified as P. aeruginosa. It was found that the optimum culture conditions for protease production in submerged culture was in the tryptic - soya broth medium at 37° C with pH 8 for 48 hours. In addition, the study i
... Show MoreIn this research, anti-reflection coatings consisting of single and double layers were designed in the IR (8 – 14μm) region to reduce the unwanted reflections of germanium (Ge) substrate. The reflectance of Ge substrate was about 36% per surface. These values were reduced reasonably by using single and double layer anti-reflection. The used layers were promoted in their performance by changing their thickness and refractive indices. The results indicated that the suggested structures are very efficient in reducing the reflectance of the Ge substrates in the selected region. The performance of the double layer antireflection coatings of ZnSe, BaF2 and BiF3 on Ge substrates is presented.
Objective:This study involved synthesis of a new series of different five-membered heterocyclic derivatives, testing their antioxidant activity, and examining their potential in vitro antimicrobial agents. Methods: The synthesis of the derivatives involved a three-step process. Initially, succinyl chloride was reacted with methanol, followed by a reaction with 80% hydrazine hydrate through a nucleophilic addition-elimination mechanism, resulting in the formation of succinohydrazide (I). This compound was then employed as a precursor for the synthesis of Schiff bases (II), and (III) by reacting it with m-nitro benzaldehyde and p-nitro benzaldehyde. Following this, a ring closure reaction was applied using thioglycolic acid, glycolic acid,
... Show MoreThe increasing amount of educational data has rapidly in the latest few years. The Educational Data Mining (EDM) techniques are utilized to detect the valuable pattern so that improves the educational process and to obtain high performance of all educational elements. The proposed work contains three stages: preprocessing, features selection, and an active classification stage. The dataset was collected using EDM that had a lack in the label data, it contained 2050 records collected by using questionnaires and by using the students’ academic records. There are twenty-five features that were combined from the following five factors: (curriculum, teacher, student, the environment of education, and the family). Active learning ha
... Show MoreThe study includ selection of six species of the fungi related to genus Pleurotus were evaluated for their ability to produce of Pleurotin, one of them, Pleurotus ostreatus (P.11) was isolated and identified in the present study. Pleurotin was detected by Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). The maximum absorption of Pleurotin was 1.6 nm at 250 nm. Pleurotin was purified with two methods using chloroform and ethyl acetate, the results showed the ethyl acetate was more efficient in pleurotin production resulting in 14.6 μg/ml compared to 9.8 μg/ml with chloroform. The local isolate, P. osteratus (P.11) showed significant high Pleurotin production (14.6 μg/ml) when was grown on the modified
... Show MoreBackground: Anti-RA33 antibodies and anti-CCP antibodies are highly specific markers for rheumatoid arthritis (RA), but are not detectable in all RA patients.
Anti-RA33 antibodies are directed to the heterogeneous nuclear ribonucleoprotein A2 (hnRNP-A2),while anti-CCP antibodies are directed to modified epitope on proteins that undergo conversion of amino acid arginine to citrullin by citrullination.
Objectives: The aim of this study was to show the correlation between anti-RA33 antibodies, anti-CCP antibodies and rheumatoid factor (RF) in terms of sensitivity and specificity for the diagnosis of rheumatoid arthritis in Iraqi patients.
Subjects and methods: This s
... Show MoreCardiovascular disease is one of the most common comorbidities associated with enlarged extremities, occurring in 60 % of patients with acromegaly. The aim of this study is to evaluate the relationship of growth hormone and insulin such as growth factor-1 with obesity, dyslipidemia, hyperglycemia, and pro-inflammatory cytokines (IL-2, IL-6, IL-10), as risk factors for cardiovascular disorder in acromegaly patients. Eighty subjects were included and categorized into two groups: 40 acromegaly patients and 40 of the control group. The results indicated weight excess, hyperglycemia, hypertension, lipid disorder, and elevated levels of interleukins (2, 6, and 10). The correlation of both GH and IGF-1 with each of weight, BMI, systolic blood p
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