This study investigated the potential of bacterial culture in bioremediation of gasoline pollutant soils. Klebsiella pneumoniae has shown a tremendous ability in bioremediation of gasoline. K.pneumoniae was isolated from three electrical generator pollutant soils with gasoline in different regions from Baghdad (Abu-Graib, Al-Khadra quarter and Al-Seleikh region. Bacteria was isolated and identified according to biochemical tests, with optimum temperature at 35°C and pH=5.
FTIR spectrum was tested the ability of the K.pneumoniae to biodegrade the gasoline according to the peak areas, which appeared and referred to degrade amino compounds at wave number 3000 cm-1 (2955.23, 2923.47) which refer to the C-H with amines compounds and decre

     The biological diversity of Klebsiella pneumoniae (K. pneumoniae) has widely been reported to be associated with pathological progress in severe nosocomial and community-acquired infections. 250 clinical specimens included sputum, urine and swabs from wound and burns samples were collected from Al-Batool Teaching Hospital (38.4%), Baqubah Teaching Hospital (61.6%) and private laboratories in Baqubah and Diyala, Iraq. Positive rates of nosocomial acquired infection were sputum 98%, urine 96%, and swabs from wound and burns 94%, while positive rates of community acquired infection were sputum 60%, urine 60%, and swabs wound and burns 30%. Positive rates of nosocomial and community acquired infections were 96% and 48%, res

Copper oxide nanoparticles (CuO NPs) were synthesized by two methods. The first was chemical method by using copper nitrate Cu (NO3)2 and NaOH, while the second was green method by using Eucalyptus camaldulensis leaves extract and Cu (NO3)2. These methods easily give a large scale production of CuO nanoparticles. X-ray diffraction pattern (XRD) reveals single phase monoclinic structure. The average crystalline size of CuO NPs was measured and used by Scherrer equation which found 44.06nm from chemical method, while the average crystalline size was found from green method was 27.2nm. The morphology analysis using atomic force microscopy showed that the grain size for CuO NPs was synthesized by chemical and green methods were 77.70 and 89.24

In the current work, Punica granatum L. peel, Artemisia herba-alba Asso., Matricaria chamomilla L., and Camellia sinensis extracts were used to prepare manganese dioxide (MnO₂) nanoparticles utilizing a green method. Energy-dispersive X-ray (EDX) analysis, Fourier Transform Infrared Spectroscopy (FTIR) analysis, and Filed emission-scanning electron microscopy (FE-SEM) analysis were used to evaluate the produced MnO₂ NPs. FE-SEM pictures demonstrated how agglomerated nanoparticles formed. According to FE-SEM calculations, the particle size ranged from 18.7-91.5 nm. FTIR spectra show that pure Mn-O is formed, while EDX results show that Mn and O are present. The ability to suppress biofilm growth in the produced MnO

Klebseilla pneumoniae possesses many virulence factors and survival strategies to persist and overcome host defenses; one of these strategies is biofilm formation. Therefore, the aims of this study was to determine the antibacterial and antibiofilm effect of Rosmarinus officinelis L. essential oil (EO) and its effect on the genes encoding of fimbrial adhesions. The antimicrobial activity was investigated by MIC. The ability to form biofilm as well as inhibition of initial cell attachment and biofilm formation was performed. PCR was carried out to detect fimH-1 and mrkD genes of type 1 and type 3 fimbrial adhesions at different time of incubation. The study revealed that MIC value of EO was 104 μg/ml on 24 (83%) of isolates, 93% of them

Klebsiella pneumoniae has been found in the urinary tract of some bladder cancer patients. Bacterial presence within tumor tissue may affect the tumor-microenvironment and consequently influence cancer behavior, development, and treatment response. This study investigated mesenchymal and stemness transdifferentiation of bladder cancer cell line due to environmental stress of K. pneumoniae. Cultures of urothelial bladder cancer cell line (T24) were infected with K. pneumoniae with different multiplicity of infection (MOI) for two and four days. Transdifferentiation-associated features were morphologically assessed.

Moreover, transdifferentiation markers were estimated using Q-PCR and immunohistoc

One hundred and nine clinical lactose fermenter isolates were collected from different samples (urine, stool, wound swab, blood, and sputum) , in a period from February 2014 till May 2014. All samples gathered from Alyarmok laboratories, Alkadimiya laboratories, and Baghdad teaching laboratories which are situated at Baghdad city. Fifty three (48,62%) isolates were identified as Klebsiella pneumoniae depending on microscopic characterization , conventional biochemical tests and then the identification confirmed with API 20E system . The rest of 56(51, 38%) isolates represented other bacteria.Susceptibility test was achieved to all fifty-three K. pneumoniae isolates using five antibiotic disks (Ceftazidime, Ceftriaxone, Cefotaxime, Imipen

Twenty-seven S. aureus isolates were obtained from patients referring various hospitals in Baghdad. Only 17 isolates produced DNase. SNase was extracted and purified from Saphylococcus aureus 3 isolate since it produces the largest zone of clearance on DNase agar. Nevertheless, only those phenotypically-producer of DNase harboured nuc gene. Present study revealed that the crude enzyme had a specific activity of 50.66 unit/mg; while it reached 241 unit/mg after ion exchange chromatography using carboxymethyl cellulose column. SDS-PAGE showed a single sharp band with an approximately 16.8 kDa molecular weight. A matter indicates that the enzyme is consistently pure. Results proved that SNase was able to significantly (P< 0.05) reduce th

There is not enough studies about bacterial contamination of air condition system in the cars and houses, bacterial detection of such   surrounding is necessary for the human environment.

The object of recent study was to evaluate the level of bacterial contamination in air conditioner in cars and houses in Baghdad city, Iraq.

Air samples were taken indoor from cars and house air conditioner   in the Baghdad city.  The result indicated that gram positive bacteria more than gram negative bacteria in air conditioner. Air condition of cars (20-500 CFU) was more contaminated than of houses (10-100 CFU).

Bacillus was the most frequently bacterial isolates genus with recovery rate B