The utilization of metal oxide nanoparticles, especially zinc oxide, is of a great importance in the medical field because of its physical and chemical properties as well as its antimicrobial potential effects. In our study, the ZnO nanoparticles were synthesized by the precipitation method where pH=14. ZnO nanoparticles were characterized by ultraviolet–visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and atomic force microscope (AFM). Antifungal activity of the ZnO was tested against candida albicans. The results showed that C. albicans (15 samples) became resistant to the fungal activity after testing its sensitivity to several types of fungal antibiotics. UV-vis spectroscopy, XRD, TEM and AFM showed that this precipitation synthetic method can produce a good quality of ZnO nanoparticles with sizes in the range of nanometer scale. In ZnO NPs (calcined), the inhibition zone diameter of C. albicans was 11 mm at a ZnO NPs concentration of 800 mg/ml, while no inhibition zones were formed at the other concentrations (0.25, 0.5, 1, 2, 10, 50 and 500 mg/ml). Using ZnO NPs (not calcined), the inhibition zone diameter of C. albicans was 24 mm at a concentration of 800 mg/ml, while no inhibition zones were observed at the other concentrations (0.25, 0.5, 1, 2, 10, 50 and 500 mg/ml). In addition, a toxicity test was performed on mice and proved that ZnO NPs are effective against C. albicans with a toxic effect on liver and spleen cells in rats. The aim of this research was to characterize the in vitro activity of ZnO nanoparticles synthesized by the precipitation method against C. albicans of human male pleural fluid using the well diffusion method as well as their toxic effects on both liver and spleen cells in mice.
Background: Recently increasing number of people are using mouthwashes for general and oral care while the primary appeal of a mouthwash is an aid to breath freshness and cleansing the mouth, the majority of mouthwashes also claim to have antiseptic properties. The aim of this study is to determine the antimicrobial effectiveness of eight types of mouthwashes against Streptococcus mutans, Staphylococcus aureus and Candida albicans in vitro. Materials and methods: Agar diffusion technique was used to evaluate the antimicrobial activity of eight types of mouthwashes against Streptococcus mutans, Staphylococcus aureus and Candida albicans isolated from the oral cavities of patients attending dental clinics at college of dentistry - Baghdad Uni
... Show MorePolyacrylamide Solutions of different concentrations (0.2, 0.4, 0.6, 0.8, 1.0 %) of Ag nanoparticles and ZnO nanoparticles were prepared, the viscosities and surface tension were measured for all solutions, where measurements indicated an increase in these properties with increased concentration, where the relative viscosity of polyacrylamide/zinc nanoparticles increased from 1.275 to 2.243, and the relative viscosity of polyacrylamide/silver nanoparticles increased from 1.178 to 1.934. Viscosity is significant parameters during electrospinning process. While the surface tension of the polyacrylamide/zinc nanoparticles has changed from 0.0343 Nm-1 to .0.0.0 Nm-1 and changed from .0.000Nm-1 to.0.0.0 Nm-1. Also the constants KH and KK were
... Show MoreCopper with different concentrations doped with zinc oxide nanoparticles were prepared from a mixture of zinc acetate and copper acetate with sodium hydroxide in aqueous solution. The structure of the prepared samples was done by X-ray diffraction, atomic force microscopy (AFM) and UV-VIS absorption spectrophotometer. Debye-Scherer formula was used to calculate the size of the prepared samples. The band gap of the nanoparticle ZnO was determined by using UV-VIS optical spectroscopy.
Zinc Oxide transparent thin films (ZnO) with different thickness from (220 to 420)nm
±15nm were prepared by thermal evaporation technique onto glass substrates at 200 with
the deposition rate of (10 2) nm sec
-1
, X-ray diffraction patterns confirm the proper phase
formation of the material. The investigation of (XRD) indicates that the (ZnO) film is
polycrystalline type of Hexagonal and the preferred orientation along (002) plane. The Optical
properties of ZnO were determined through the optical transmission method using ultraviolet-visible spectrophotometer with wavelength (300 – 1100) nm. The optical band gap values of
ZnO thin films were slightly increased from (2.9 - 3.1) eV as the film thickn
Histologic changes were studied and physiological dosage crude alcoholic extract of seeds of the fenugreek plant for male mice eggs in different concentrations after oral to study testicular tissue and culverts where reason Abstract significant decrease
These dust designed to identify the extent of the impact of alcohol Almstkhalss saponin from fenugreek seeds on fertility in male mice eggs by tracking some physiological changes and tela that may occur to some members of the device Altcatherthe
Laser ablation of a silver target immersed in distilled water using Nd:YAG laser with a fundamental wavelength of 1064nm was carried out to fabricate silver nanoparticles (Ag NPs) with different laser energy in the presence and absence of magnetic field. UV-Visible spectrum showed that the nanoparticles are almost spherical in shape. The number of Ag NPs increased by increasing laser energy while their particle size was reduced by increasing laser energy without magnetic field. In the presence of magnetic field, the size of Ag NPs increased slightly by increasing laser energy. According to AFM results, the presence of magnetic field did not affect the average diameter of Ag NPs. The presence of a magn
... Show MoreComparative study on tb.e activity of phospholipase enzyme which is produced by yeastآ cum/ida albicansآ آ into different culture media that contain phospholipids as substrates. The agar containing substrate (L-aآ phosphatidyl inositol) was used .
The agar containing lecithin as other substrate for comparision was also
used.
The second culture media was found the best for enzyme activity which
was measured by precipitation zone .
Cutaneous leishmaniasis (CL) also known under local names like (tropical sore, oriental sore and Baghdad sore) is the most common form of leishmaniasis. It is a public health and a social problem in many developing countries. The Old World disease primarily is caused by Leishmania major in dry desert areas and Leishmania tropica in urban areas. Recently, metal oxide nanoparticales have been versatile platforms for biomedical applications and therapeutic interventions. There is an urgent need to develop new types of antileishmanial agents instead of classical drug (pentostam), especially, when its efficacy showed a decline towards some strains of Leishmania. Therefore, the present work was aimed to evaluating antileishmanial activity of z
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Detection of virulence gene agglutinin-like sequence (ALS) 1 by using molecular technology from clinical samples (