Pseudomonas aeruginosa was isolated from various clinical samples included urine, sputum, stool, ear, wound & burn swabs. Detection of the ability of local isolates to produce staphylolysin enzyme was studied, on Tryptic soya agar + 0.2% (wt./vol.) of heat killed Staphylococcus. aureus at temperature 100oC. medium and the diameters of lysis zone ranged from 5-22mm, then the isolate P16 was chosen to extract staphylolysin A (LasA) and its specific activity reaches 8.59 unit /mg protein, whi1e the isolate P5 was chosen to extract staphylolysin D (LasD) where it's specific activity reaches 0.66 unit /mg protein since the two isolates were the most production of enzyme. Staphylolysin enzyme was extracted by cooling centrifugation and partially purified by ammonium sulphate precipitation in saturation percentage of 80%, this step was followed by Ion exchange chromatography technique by using DEAE- cellulose column, results showed that the enzymatic activity (Staphylolytic activity) of the staphylolysin A appeared in first peak with purification folds and recovery of 10.74 fold and 14.2% respectively, while the second peak appeared the activity for staphylolysin D with purification folds and recovery of 9.1 fold and 18.14% respectively.
Background: Arylesterase activity of Paraoxonase-1 (ARE-PON-1) exhibits an antioxidant role which protects lipoprotein from oxidation. It is known that ARE-PON-1 antioxidant activity associated with high density lipoprotein cholesterol (HDL-C) reduces the oxidative damage mediated by low density lipoprotein cholesterol (LDL-C). The present study was aimed to examine the level of serum ARE-PON1 in Iraqi patients with β-thalassemia minor and its relationship with lipid profile (total cholesterol (TC), HDL-C, very low density lipoprotein (VLDL-C), and LDL-C) and hematologic changes as a part of antioxidant system action. Methods: In the present study, the ARE-PON-1 activity was investigated in serum of patients with β-thalassemia minor. Resu
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Endophytic fungi live inside plants or any part of them without creating any visible pathogenic signs. Endophytic fungi are found within medicinal plants and have shown strong biologic activity, such as anticancer and antioxidant activities, as well as producing extracellular enzymes. In this study, different fungal strains were isolated from the leaves of the medicinal plant Ziziphus spina, including Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Cladosporium sp., Rhizopus sp., and Mucor sp. Extracellular enzymes have been quantified using agar plate-based methods in which fungi were grown in specified growth media to detect the enzymes produced. The results showed that A. niger has the highest ability to produce amy
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Objectives Bromelain is a potent proteolytic enzyme that has a unique functionality makes it valuable for various therapeutic purposes. This study aimed to develop three novel formulations based on bromelain to be used as chemomechanical caries removal agents. Methods The novel agents were prepared using different concentrations of bromelain (10–40 wt. %), with and without 0.1–0.3 wt. % chloramine T or 0.5–1.5 wt. % chlorhexidine (CHX). Based on the enzymatic activity test, three formulations were selected; 30 % bromelain (F1), 30 % bromelain-0.1 % chloramine (F2) and 30 % bromelain-1.5 % CHX (F3). The assessments included molecular docking, Fourier-transform infrared spectroscopy (FTIR), viscosity and pH measurements. The efficiency
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Polyphenol oxidase (PPO) is an enzyme containing copper, presents in various fruits and vegetables. It is responsible for the browning reactions when the cells are damaged during handling. The best conditions for extraction of polyphenol oxidase from banana peel was by using an extraction buffer containing phosphate buffer (0.05 M, pH 7), 0.01 M ascorbic acid and 0.5% polyethylene glycol, with extraction ratio 1:4 (w:v) for one minute by using blender. The enzyme activity was measured spectrophotometrically at 425 nm. PPO was studied to prevent the browning of banana peel which results in the loss of their marketability. The aim of this study was to determine the optimum conditions for polyphenol oxidase extraction from banana peel.
The removal of direct blue 71 dye from a prepared wastewater was studied employing batch electrocoagulation (EC) cell. The electrodes of aluminum were used. The influence of process variables which include initial pH (2.0-12.0), wastewater conductivity (0.8 -12.57) mS/cm , initial dye concentration (30 -210) mg/L, electrolysis time (3-12) min, current density (10-50) mA/cm2 were studied in order to maximize the color removal from wastewater. Experimental results showed that the color removal yield increases with increasing pH until pH 6.0 after that it decreased with increasing pH. The color removal increased with increasing current density, wastewater conductivity, electrolysis time, and decreased with increasing the concen
... Show MoreAn experiment was conducted to study the effect of four isolates of Pseudomonas spp. on the growth of two plants Radish & Cowpea and on the concentrations of macro elements & microelements . This experiment included two parts , the 1st. part included isolation and characterization of 4 isolates of Pseudomonas bacteria from local Iraqi soils Baghdad . The 2nd part included planting two plants Radish & Cowpea in plastic pots size 5Kg -soil in the green house Biology Dept. College of Science , after planting we added the isolates to the pots , and after 50 days, the growth parameters length , fresh and dry weight , percentage of ger
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