To elucidate the anti- Methicillin resistance Staphylococcus aureus (MRSA) effect of pomegranate alone and in combination with moxifloxacin fluoroquinolone. A total of five clinical isolates of MRSA (ATCC 43300) were used in the study. Disc diffusion method was used to determine the anti-MRSA effect of pomegranate and/or moxifloxacin by using Mueller-Hinton agar. Minimal inhibitory concentration (MIC), fractional inhibitory concentration (FIC) of moxifloxacin and pomegranate were calculated, the dynamic picture of the bactericidal effect of pomegranate and/or moxifloxacin was determined. SPSS version 20.00 was used for data analysis. Zone of inhibition (ZOI) of moxifloxacin was 19.67±4.84mm which was not significant compared with pomegrana

In accordance with epidemic COVID-19, the elevated infection rates, disinfectant overuse and antibiotic misuse what led to immune suppression in most of the population in addition to genotypic and phenotypic alterations in the microorganisms, so a great need to reevaluate the genetic determinants that responsible for bacterial community (biofilm) has been raised. A total of 250 clinical specimens were obtained from patients in Baghdad hospitals and streaked on Mannitol salt agar medium. The results revealed that 156 isolates appeared as round yellow colonies, indicating that they were mostly identified as Staphylococcus aureus from 250 specimens. The antibiotic resistance pattern of the isolates for methicillin 37.17% (n=58), Amoxic

The emergence and spread of methicillin-resistant Staphylococcus aureus (MRSA) was a public health problem worldwide that causes nosocomial and community infections. Forty three isolates (71.66%) were characterized as S.aureus, were isolated from 60 different clinical specimens (blood, nose, wound, urine and vaginal) collected from patients from different hospitals of Baghdad. All isolates were resistant (100%) to Aztreonam, Carbenicillin, Cifixime, Cefoxitin, Ceftazidime, and showed high resistance to each of Methicillin, Oxacillin, Ampicillin and Penicillin . the MRSA isolates were typed based on (SCCmec) typing ,the result revealed that SCCmecIVa was the most common in isolates (41.86%), following type IVc (20.93%), type II(16.27%). V

In this study 77 human isolates of Staphylococcus aureus were obtain from different clinical sources. The results showed that the number of isolates producing α-hemolysin was 32 isolates (41.55%) , while non- hemolysin producing was 45 isolates (58.45%) .The minimum inhibitory concentration (MIC) of methicillin which were 32 micrograms \ ml. The effect of CD-Cholesterol ,Cholesterol ,Cyclodextran(CD) ,Methicillin and Phosphate Buffer Saline(PBS) on α- hemolysin activity was study and the hemolytic Titer was:8 ، 32.768 ,65.536 ,
140.737.488.355.32 , 4.961408E + 25 respectively, while the effect of the same effect with Titer 67.108.864 and low Titer with PBS 3.96140812E + 25. The α – hemolysin toxin was partialy purified by ammoni

 Background: The resistance to antimicrobial factors is an increasingly global problem worldwide, especially among nosocomial bacteria. Staphylococci have become one of the common causes of nosocomialinfections. Multi drug-resistant staphylococci pose a growing problem for human health.
ObjectiveThis study was carried out to evaluate the association between the antibiotic susceptibility patterns and the msrA Erythromycin-Resistant gene in staphylococci isolates obtained from various clinical samples of patients.
Methods: A total of 25 staphylococci clinical isolates were collected from the central Health Lab. DNA from each sample was extracted and The gene implicated in resistance to erythromycin (msrA) was amplified using PCR

Eight isolates of methicillin resistance Staphylococcus aureus(MRSA) (SA40,SA32,SA30,SA13,SA10,SA36,SA3 and SA7) with different resistance phenotypes  to macrolides , lincosamides and streptogramins Were used to detect theexpression of msrA, msrB, and linA/linA’genesby using  real time polymerase chain reaction before and after treatment with antibiotics (erythromycin , clarithromycin , clindamycin and lincomycin) calibrated with triosphosphateisomerase.There highst expression of these genes was after 18 hours. It was  an induction in the expression of msrA gene in isolates (SA40,SA32,SA30 and SA13) in presence of erythromycin,however,the  isolates showed reduction in expression l

This study aimed to detect of contamination of milk and local soft cheese with Staphylococcus aureus and their enterotoxins with attempt to detect the enterotoxin genes in some isolates of this bacteria. A total of 120 samples, 76 of raw milk and 44 of soft cheese were collected from different markets of Baghdad city. Enterotoxins in these samples were detected by VIDAS Set 2 system and it was found that enterotoxin A is present in a rate of 44.74% in milk samples and in a rate 54.50% in cheese samples. While other enterotoxins B, C, D, E were not found in any rate in any samples.
Through the study 60 isolates obtained from milk and cheeses were identified as Staphylococcus aureus by cultural, morphological and biochemical test by u

The pathogenicity resulting from Staphylococcus aureus infection has remarkable importance as one of the community-associated bacterial infections, due to the virulent ability of these bacteria to produce biofilms. This study was designed to detect biofilm production in clinical isolates from samples of wounds and urinary tract infections. The expression levels of the icaA gene that is responsible of slime layer production in biofilms was compared in isolates with different biofilm producing capabilities. Fifty seven samples that included 32 samples from urine and 25 samples from wounds were collected from Alwasti Hospital, Al-Kindi Teaching Hospital, and Alzahraa Clinic, Baghdad, Iraq. The bacteria was identified accor