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Expression Of Xylose Reductase Enzyme From Spathaspora passalidarum In Saccharomyces cerevisiae.
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Baker’s yeast (Saccharomyces cerevisiae) has been genetically engineered to
ferment the pentose sugar xylose present in lignocellulosic biomass. One of the
reactions controlling the rate of xylose utilization is catalyzed by xylose reductase
(XR).The current study describes xylose reductase from Spathasporapassalidarum
with NADH preference. According to JGI site the gene coding for this enzyme
contains 954 nucleotides and it consists of 317 amino acids. The restriction sites for
the enzymes SacII and NotI located on the 5P
´
Ptermini for both the forward and
reverse specific primers were designed using Lasergen 9.0 program. The genomic
DNA was isolated and purified from S .passalidarum. Polymerase chain reaction
(PCR) was used to amplify this gene. The amplified gene was cloned into pSN303
plasmid resulting of the pYIM1 plasmid and then transformed into Escherichia coli.
This plasmid was reisolated from E. coli, sequenced,and finally transformed into S.
cerevisiae. The yeast transformants carrying pYIM1 plasmid named YJTY1. The
specific activity of enzyme was 1.55 and 0.48 U/mg on NADH and NADPH
respectively for YJTY1. This enzyme has a natural preference for NADH which
makes it a good candidate for combination with NADP
+
Pdependent xylitol
dehydrogenase which may enable S. cerevisiae to utilize xylose under anaerobic
conditions and convert it to ethanol.

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Publication Date
Tue Dec 29 2020
Journal Name
Iraqi Journal Of Science
The Synergistic Effects of Chitosan-Alginate Nanoparticles Loaded with Doxycycline Antibiotic Against Multidrug Resistant Proteus Mirabilis, Escherichia Coli and Enterococcus Faecalis
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     This study focused on the synthesis of chitosan-alginate (CH-ALg) nanoparticles by ionotropic gelation technique using sodium alginate and calcium chloride. The particle size of the synthesized nanoparticles was confirmed by atomic force microscope (AFM) and it was 61.9 nm. While the nature of functional groups present in chitosan nanoparticles was determined by FT-IR analysis. The antibacterial activity of chitosan-alginate was tested against multidrug resistance (MDR) gram- positive (Enterococcus faecalis) and gram-negative (Proteus mirabilis) bacteria. The results showed a significant effect against MDR isolates. The nanoparticles were loaded with the antibiotic doxycycline in order to improv

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Publication Date
Mon Mar 08 2021
Journal Name
Baghdad Science Journal
The Alvezaúah properties of crude oil and associated water and the relationship with qualities Alheidrocorpunat resulting Fertile Mkami and central Iraq
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Conducted Althilelat chemical models of crude oil back to the reservoir Fertile from the fields of Baghdad and Kut and models of crude oil back to the reservoir ??????? of Haklbe Tikrit and Baghdad were calculated their properties Alvezaúah Kalkthaqh and weight, quality and degree of August j (API) and know the quality Nfothma that was light or heavy and make the comparison between Alinvtin also conducted chemical analyzes of the two models of Almia associated with each of the oil above Almkmnin and measured Ktvthma and Zojithma and concentrations of some dissolved salts in them and clarify the relationship between the oil reservoir and water associated with oil fields...

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Publication Date
Fri Mar 31 2023
Journal Name
Oncology And Radiotherapy
The Effect of Green Low Laser (LLL) on the white blood cells on platelet on people on brain and prostate cancer
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The effect of Low-Level Laser (LLL) provided by green semiconductor laser with an emission wavelength of 532 nm on of human blood of people with brain and prostate cancer has been investigated. The effect of LLL on white blood cell (WBC), NEUT, LYMPH and MONO have been considered. Platelet count (PLT) has also been considered in this work. 2 ml of blood sample were irradiating by a green laser of the dose of 4.8 J/cm2. The results suggest a potential effect of LLL on WBC, PLT, NEUT, LYMPH, and MONO of people with brain and prostate cancer Key words: white blood cell , platelet , low-level laser therapy

Publication Date
Wed Jan 12 2022
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Evaluating the Impact of Pharmacist Counselling for Asthmatic Children at Karbala Teaching Hospital for Children: An Interventional Prospective Study (Conference Paper )#
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Asthma is one of the most common chronic, non-communicable diseases affecting children worldwide. The estimated prevalence of pediatric asthma in Iraq is 15.8%. Physiologic, inflammatory and structural factors contribute to the development of asthma. Assessment and monitoring of asthma control can be done by a validated children asthma control test (CACT). Management of asthma must address three components which are an appropriate management plan, the most appropriate medication if necessary, and the use of safe and effective medication. The management plan should consider patient counseling and education about the definition of asthma, signs, and symptoms, the pathophysiology of asthma, common triggers for asthma and how can avoid them,

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Publication Date
Tue Aug 22 2023
Journal Name
Advances In Structural Engineering
Experimental and finite element analysis of reinforced concrete multi-cell box girders retrofitted with carbon fiber reinforced polymer strips under torsion
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This study expands the state of the art in studies that assess torsional retrofit of reinforced concrete (RC) multi-cell box girders with carbon fiber reinforced polymer (CFRP) strips. The torsional behavior of non-damaged and pre-damaged RC multi-cell box girder specimens externally retrofitted by CFRP strips was investigated through a series of laboratory experiments. It was found that retrofitting the pre-damaged specimens with CFRP strips increased the ultimate torsional capacity by more than 50% as compared to the un-damaged specimens subjected to equivalent retrofitting. This indicated that the retrofit has been less effective for the girder specimen that did not develop distortion beforehand as a result of pre-loading. From

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Publication Date
Mon Jul 01 2019
Journal Name
Ceramics International
Surface structural features and optical analysis of nanostructured Cu-oxide thin film coatings coated via the sol-gel dip coating method
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Publication Date
Thu Mar 31 2022
Journal Name
Iraqi Geological Journal
Geological Model of the Khabour Reservoir for Studying the Gas Condensate Blockage Effect on Gas Production, Akkas Gas Field, Western Iraq
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The Khabour reservoir, Ordovician, Lower Paleozoic, Akkas gas field which is considered one of the main sandstone reservoirs in the west of Iraq. Researchers face difficulties in recognizing sandstone reservoirs since they are virtually always tight and heterogeneous. This paper is associated with the geological modeling of a gas-bearing reservoir that containing condensate appears while production when bottom hole pressure declines below the dew point. By defining the lithology and evaluating the petrophysical parameters of this complicated reservoir, a geological model for the reservoir is being built by using CMG BUILDER software (GEM tool) to create a static model. The petrophysical properties of a reservoir were computed using

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Publication Date
Sun Sep 01 2024
Journal Name
Results In Engineering
Push-out test of eco-friendly steel-concrete–steel composite sections enhanced by polypropylene fibers: An experimental study and statistical analysis
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Steel-concrete-steel (SCS) structural element solutions are rising due to their advantages over conventional reinforced concrete in terms of cost and strength. The impact of SCS sections with various core materials on the structural performance of composites has not yet been fully explored experimentally, and in this work, both slag and polypropylene fibers were incorporated in producing eco-friendly steel-concrete-steel composite sections. This study examined the ductility, ultimate strength, failure modes, and energy absorption capacities of steel-concrete-steel filled with eco-friendly concrete, enhanced by polypropylene fiber (PPF) to understand its impact on modern structural projects. Eco-friendly concrete was produced by the partial

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Publication Date
Sat Jan 01 2022
Journal Name
International Journal Of Applied Science And Engineering
The effect of model scale, acceleration history, and soil condition on closed-ended pipe pile response under coupled static-dynamic loads
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This paper analyzes the effect of scaling-up model and acceleration history on seismic response of closed-ended pipe pile using a finite element modeling approach and the findings of 1 g shaking table tests of a pile embedded in dry and saturated soils. A number of scaling laws were used to create the numerical modeling according to the data obtained from 1 g shake table tests performed in the laboratory. The current study found that the behaviors of the scaled models, in general have similar trends. From numerical modeling on both the dry and saturated sands, the normalized lateral displacement, bending moment, and vertical displacement of piles with scale factors of 2 and 35 are less than those of the pile with a scale factor of 1 and the

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Publication Date
Mon May 22 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Univariate and Simplex Optimization for the Spectrophotometric Determination of Cimetidine and Erythromycin ethylsuccinate Drugs Using Bromothymol Blue Via Ion-Pair Formation
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    The ion-pair formation method has been applied for the spectrophotometric determination of Cimetidine and Erythromycin ethylsuccinate, in bulk samples and in dosage form. The methods are accurate, simple, rapid, inexpensive and sensitive depending on the extraction of the formed ion-pair with brompthymol blue (BTB) as a chromogenic reagent in chloroform, use phthalate buffer of pH 5.5 and 4.0 for Cimetidine and Erythromycin ethylsuccinate respectively.  The formed complexes show absorbance maxima at 427.5 nm and 414.5 nm for Cimetidine and Erythromycin ethylsuccinate respectively against reagent blank. The calibration graphs are linear in the ranges of 0.5-15 µg.mL-1 with detection limit of 0.222 µg.mL-1 for

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