Preferred Language
Articles
/
ijs-12066
Extraction and Purification of Vibrio Cholerae Enterotoxin and Study its Cytopathic Effect on Some Mice Organs
...Show More Authors

Vibrio cholerae enterotoxin was extracted by cooling centrifugation and filtration
with milipore filter (0.22um) and was purified by using Sephacryl –S- 6 gel
filtration,the content of protein was estimated . The results showed protein
concentration was 28.5 microgram/ml,the present of enterotoxin was detected by
infant suckling mouse method.
.The cytopathic effect of enterotoxin was studied by injecting a number of mice with
purified enterotoxin, It was found caused shortening the villi of the intestine at
concentration 55 and 45 ug /ml of purified enterotoxin, while the effect on liver
showed degenerative change with necrosis at 55 ug/ml of enterotoxin and caused
necrosis and infiltration of inflammatory cells at 45 ug /ml, the effect on spleen
tissue was represented by widening of white pulp, and reduction or disappearance of
red pulp also inflammatory cells wre found i.e. lymphocyte and hypoplasia of white
pulp at the two concentrations of the enterotoxin .

View Publication Preview PDF
Quick Preview PDF
Publication Date
Sun Jul 02 2017
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Isolation and Identification of Clostridium perfringens and its Enterotoxin in Food poisoning Patients
...Show More Authors

Background: Clostridium perfringens enterotoxin (CPE), which is one of the most common cause’s foodborne illnesses and contribute to diarrhea that is associated with broadspectrum antibiotic treatment.
Objectives: This study focuses on diagnosis of Clostridium perfringens enterotoxin (CPE) from patients suffering from food poisoning and diarrhea associated with antibiotic treatment cases in stool samples and to determine the resistance of isolated against antibiotics.
Methods: Samples were taken during the period of first of June 2015 until the end of April 2016 from Baghdad hospitals. Enzyme Linked Immunosorbent Assay (ELISA) was used to detect Clostridium perfringens enterotoxin in stool samples. Api 20A kit and culture to conf

... Show More
View Publication Preview PDF
Crossref
Publication Date
Fri Jan 26 2024
Journal Name
Iraqi Journal Of Science
The Extraction and Partial Purification of Wedelolactone from Local Eclipta alba Plant
...Show More Authors

Abstract:
Eclipta alba is a weed growing in damp, moist puddles distributed in the tropical and subtropical regions, so the most weight of the plant is water, which reached in to 90%. The extraction method by using different solvents (Methanol, Ethanol, Hexane and Aqueous) showed, the best yield with methanol reached to 76%, and the yield decreased with ethanol and hexane reached 55% and 53% respectively, while the minimum yield observed with aqueous hot and cold extraction reached 11% and 5% respectively. The phytochemical compound characterization showed the compounds (Coumarines , Flavones ,Volatile Oil ,Tannins , Saponines , Glycosides ,Carbohydrates ,Alkaloids , Resins) with different percentage. The thin layer chromatography det

... Show More
View Publication Preview PDF
Publication Date
Thu Oct 02 2014
Journal Name
Basrah Journal Of Science
Extraction and partial purification for fimbriae from Proteus mirabilis and study their role in adhesion to uroepithlial cells
...Show More Authors

From 211 urine samples, Gram negative bacteria were isolated from only 61 urine samples with isolation percentage 28.9%. Escherichia coli were isolated percentage 70.49% while Klebsiella pneumoniae and Psendomonas aeruginosa were 8.19% and 6.55%, respectively.Proteus spp. Were isolated from 9 (14.75%), P. mirablis and P. vulgaris were isolates percentage 11.47% and 3.27%, respectively. Uroepithelial Cell Adhesin (UCA) fimbriae expression by P.mirabilis isolates was detected by the high capacity to adhesion to human uroepithetial cells, the isolate p.mirabilis U7 was adhesion to human uroepithelial cells mean no.30.2 bacteria/cell when grown on luria broth at 37C for 24h, but then grown it’s on luria agar at 37C for 24h the adhesion

... Show More
View Publication Preview PDF
Publication Date
Sun Dec 01 2019
Journal Name
Baghdad Science Journal
Extraction, Purification and Characterization of Peroxidase from Pseudomonas aeruginosa and Utility as Antioxidant and Anticancer
...Show More Authors

        Peroxidase is a class of oxidation-reduction reaction enzyme that is useful for accelerating many oxidative reactions that protect cells from the harmful effects of free radicals. Peroxidase is found in many common sources like plants, animals and microbes and have extensive uses in numerous industries such as industrial, medical and food processing. In this study, P. aeruginosa was harvested to utilize and study its peroxidases. P. aeruginosa was isolated from a burn patient, and the isolate was verified as P. aeruginosa using staining techniques, biochemical assay, morphological, and a sensitivity test. The gram stain and biochemical test result show rod pink gram-ne

... Show More
View Publication Preview PDF
Scopus (3)
Scopus Clarivate Crossref
Publication Date
Sun Mar 13 2011
Journal Name
Baghdad Science Journal
Extraction and purification of L-Asparaginase II from local isolate of Proteus vulgaris
...Show More Authors

Forty one isolates of genus Proteus were collected from 140 clinical specimens such as urine, stool, wound, burn, and ear swabs from patients of both sex. These isolates were identified to three Proteus spp. P. mirabilis, P. vulgaris and P. penneri .The ability of these bacteria to produce L-asparaginase II by using semi quantitative and quantitative methods was determined. P. vulgaris Pv.U.92 was distinguished for high level of L-asparaginase II production with specific activity 1.97 U/mg. Optimum conditions for enzyme production were determined; D medium with 0.3% of L-asparagine at pH 7.5 with temperature degree 35°C for incubation. Ultrasonication was used to destroy the P. vulgaris Pv.U.92 cells then ASNase II was extracted and pu

... Show More
View Publication Preview PDF
Crossref
Publication Date
Wed Jan 02 2008
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The Vibrio Cholera
...Show More Authors

Introduction:
Vibrios are gram negative rods bacteria that are all widely distributed in nature the vibrose are found in marine and surface waters. They are curved aerobic rods
and are motile, possessing a polar flagellum. V.vibrios sero group 01 and related Vibrios cause cholera cause sepsis or enteritis Vibrio cholerae The epidemiology
of cholera closely parallels the recognition of V.cholerae transmission in water and the development of sanitary water system (1,2,6,9). V. C is a comma-shaped, curved rod 2-4 long .It is actively motile by means of polar flagellu on prolonged cultivation. Vibros may become straight rod that  resemble the gram-negative enteric bacteria (1,2,6,9). V.C. grow well

... Show More
View Publication Preview PDF
Crossref
Publication Date
Sun Mar 02 2008
Journal Name
Baghdad Science Journal
Extraction and Purification of Indole aectic acid from locale isolate Fusarium oxysporum(F2)
...Show More Authors

Indole acetic acid (IAA) produced from F. oxysporum (F2) was purified by several steps included extraction by cold ethyl acetate ; Column chromatography using silica gel and TLC chromatography . The pure indole acetic acid (IAA) which produce by F. oxysporum (IAA) was tested by ultraviolet spectra at (200-300)nm ; and appear that the maximum absorbance at 229nm , the high performance liquid chromatography (HPLC) used to test the purity of the indole acetic acid and the results showed one peak at appearance time 3.822 min

View Publication Preview PDF
Crossref
Publication Date
Sun Jan 02 2011
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Histopathological and enzymatic study on the effect of Aspergillus fumigatus in mice
...Show More Authors

Background: - Aspergillus fumigatus is a ubiquitous and opportunistic fungal pathogen causing sever invasive aspergillosis, an important source of morbidity and mortality in
immunocompromised hosts (human and animals) .The present study was conducted to obtain more clarification about the toxicity and histopathological effects of this pathogen in albino mice
Material and Methods :- fourteen male mice( 6-10) weeks old ,( 20 – 28) gm weight , divided into two groups , test group ( n= 8) , intranasally infected with 25μl (710 conidia / ml normal
saline ) of A. fumigatus for 7 successive days , compared with uninfected group ( n=6) . the weight of all animals were measured at the beginning and at the end of th

... Show More
View Publication Preview PDF
Crossref
Publication Date
Sun Mar 06 2011
Journal Name
Baghdad Science Journal
Isolation and Identification of Vibrio cholerae causes Epidemic cholera 2007 in Iraq by Rapid Method (Immunochromatographic one step rapid visual test) and comparing it with the traditional Bacterlogical Methods
...Show More Authors

This study was for searching for Cholera Bacteria serotype which causes epidemiology Cholera in the 2007 in a fast method which contains (Rapid Visual Test) (Crystal V.C.) which was used for the first time in Iraq to diagnosis of Cholera Bacteria & compared with the traditional bacteriology method. The Cholera disease is one of the most dangerous epidemiological diseases which lead to death with a percentage of (50 – 70) % in the severe cases for untreated patients . For this purpose, 100 samples of stool from the patients from a (13) hospitals in Baghdad Governorate in the period from August to the end of December. The Cholera was diagnosis in two methods, 1st method was the fast method using the nitrocellulose which is coated with anti-

... Show More
View Publication Preview PDF
Crossref
Publication Date
Wed Aug 13 2014
Journal Name
Journal Of Biotechnology Research Center
In Vivo Study for Measuring the Toxicity of Heat Stable Enterotoxin (a) Produced by Enterotoxigenic Escherichia coli in Mice
...Show More Authors

This research was conducted to measure the safety of heat stable enterotoxin a (STa) produced by enterotoxigenic Escherichia coli, through studying its toxic effect on mice since it showed a promising effect in reducing the proliferation of colorectal cancer cells. The cytogenetic effect was determined after giving five different doses (100, 200, 400, 800 and 1600)μg/Kg in comparison with negative (phosphate buffer saline / PBS) and positive (mitomycin C/ MMC, at doses of 2 and 5μg/Kg) controls on mouse bone marrow cells by employing the following parameters: mitotic index, chromosomal aberrations and micronucleus, also, the serum level of liver functional enzymes (GOT, GPT, ALP) was recorded. In addition, lethal dose 50 (LD 50) with cert

... Show More