Lipoxygenase was extracted from the cup of Pleurotus ostreatus ( Jaq : Fr ) oyster mushroom for the first time in Iraq, and purified homogeneously through precipitation with 40% saturation of (NH4)2SO4 as a partial purification then loaded on DEAE-Cellulose (Diethyl amino ethyl Cellulose) ion-exchange chromatography column and then the highly active elution parts have been passed through gel filtration column with Sephacryl S-300 as a final purification with 804 (U/mg protein) specific activity, 11.32 fold of purification and 36.54% yield . The molecular weight of the enzyme was estimated to 74 KDa by gel filtration Sephacryl S-300 column and the isoelectric point for enzyme was 5.3. The optimal pH for lipoxygenase activity and stability were 8 and 6-8.5 respectively, and the optimal temperature for the activity and stability of the enzyme were 30 and 10-45 respectively. Also the activation energy necessary for Lipoxygenase to convert lionleic acid to product and for enzyme denaturalization were calculated to 9.674 and 28.087 Kilo calorie/mole respectively.
The extraction behaviour of a secondary amine (Dioctylamine) and a combination of secondary amine and tertiary amine (Tricapryl amine) toward some of the metal ions were investigated. Two types of diluents were applied, namely, mesitylene and treated Kerosene fraction. The extractability of dioctylamine and different combinations of tertiary amine and secondary amine toward uranium were investigated. The distribution coefficients and the separataion factors of uranium from the metal ions were calculated.
This study included the extraction of volatile oils from the leaves of some local Eucalyptus trees (i.e.(1)E.camldulensis ,(2) E.camldulensis , E.microtheca , E.globules , E.sideroxylem , E.krusianin. by the water distillation method. As well as exploring some of their physical properties. The results of this study reveald that the local eucalyptus trees gave a good rate of volatile oil equals to some global kinds and to the rate in India pharmacopeia. Also the results revealed that there had been concurrence between the percentages of volatile oils and refractive index and specific gravity of the plants on which the study was made to study and those included i
... Show Morehe study aimed to purify of Leucine aminopeptidase (LAP) from sera of hyperthyroidism patients and its relation to some thyroid hormones (TSH, T 3 , T 4 ) of subclinical hyperthyroidism and hyperthyroidism patients with lipid peroxidation levels that may be play a role in this diseases. Specimens were collected during the time from Nov 2017 until Jan 2018 from Endocrine and Diabetes Center, blood samples were collected from fifty healthy control and one hundred patients, patients were divided into two groups consisted of (50) with hyperthyroidism and (50) with subclinical hyperthyroidism. The aged for all subjects ranged (15-60) years with body mass ranged ((25- 29) kg/m 2 . The purification is done by addition of ammonium sulfate, dial
... Show MoreMeasuring goat weight on a farm is carried out by two methods. The first method conventionally measures the chest girth (CG) and body length (BL), while the second is performed using a scale. This study aimed to facilitate the CG and BL measurements to estimate goat weight more efficiently through image processing. The endpoints of CG and BL were determined automatically using binary large object (BLOB) segmentation. The images of 120 goats were taken at three different shooting distances of 50 cm, 70 cm, and 90 cm. The statistical testing of the three scenarios in this study explained that the ideal distance to take pictures is the basis for determining whether or not the size of the circumference and BL is appropriate as a fea
... Show MoreOne of the challenging and active research topics in the recent years is Facial Expression. This paper presents the method to extract the features from the facial expressions from still images. Feature extraction is very important for classification and recognition process. This paper involve three stages which contain capture the images, pre-processing and feature extractions. This method is very efficient in feature extraction by applying haar wavelet and Karhunen-Loève Transform (KL-T). The database used in this research is from Cohen-Kanade which used six expressions of anger, sadness fear, happiness, disgust and surprise. Features that have been extracted from the image of facial expressions were used as inputs to the neural networ
... Show MoreIn this article, new Schiff base ligand LH-prepared Mn(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II), Hg(II), Pd(II), and Pt(II) materials were analyzed using spectroscopy (1 Metal: 2 LH). The ligand was identified using techniques such as FTIR, UV-vis, 1H-13C-NMR, and mass spectra, and their complexes were identified using CHN microanalysis, UV-vis and FTIR spectral studies, atomic absorption, chloride content, molar conductivity measurements, and magnetic susceptibility. According to the measurements, the ligand was bound to the divalent metal ions as a bidentate through oxygen and nitrogen atoms. The complexes that were created had microbicide activity against two different bacterial species and one type of fungus. DPPH techniques were bei
... Show MoreThe Schiff bases (1-10) were synthesized by the reaction of cefixime with aldehydes derivatives. The characterization of Schiff bases were carried out, by using spectroscopic techniques including IR, U.V – Vis, H1-NMR, EI-MS along with elemental analyses (C.H.N.).
Schiff base obtained from the reaction (Trimethoprim) with (sodiumpyruvate ) to produce the ligand [NaL], the reaction was carried out in methanol as a solvent under reflux. The prepared ligand [NaL] was characterized by FT-IR, UV-Vis spectroscopy, 1H,13C-NMR spectra, mass spectra, and melting point.A new mixed ligand complexes have been prepared between ( 8- hydroxyquinolone) and the ligand [NaL] withMn(II).Co(II),Ni(II),Cu(II), (Zn(II) ,(Cd(II)and Pd(II). All the complexes were characterized by spectroscopic methods (FT-IR, UV-Vis spectroscopy), chloride content and melting point ,molar conductance and magnetic susceptibility.These measure- ments showed octahedral geometry around(,Mn2+, Co2+, Ni2+, Cu2+, Zn2+ and Cd2+) ions and square pla
... Show Morechloride or poluacrulic acid with different primary amines to mesuring==================================638one hundred three patinents with rheumatic symptoms were include in this study and their sera tested for
Feature extraction provide a quick process for extracting object from remote sensing data (images) saving time to urban planner or GIS user from digitizing hundreds of time by hand. In the present work manual, rule based, and classification methods have been applied. And using an object- based approach to classify imagery. From the result, we obtained that each method is suitable for extraction depending on the properties of the object, for example, manual method is convenient for object, which is clear, and have sufficient area, also choosing scale and merge level have significant effect on the classification process and the accuracy of object extraction. Also from the results the rule-based method is more suitable method for extracting
... Show More