Two hundred staphylococcal isolates isolated from milk and white cheese samples, which were collected from local markets in Baghdad. The predominant species was Staphylococcus aureus 97 isolates (48.5%), followed by S.chromogenes 82 (41%) and 21 (10.5%) S.epidermidis isolates. The pattern of antibiotic susceptibility of Coagulase Positive Staphylococci (COPS) and Coagulase Negative Staphylococci (CONS) isolates to 3 antibiotics (Methicillin, Tetracyclin and Vancomycin) was determined using disc diffusion method; the results revealed that 80 S. aureus isolates (82.47%) found to be methicillin resistant (MRSA) while 8 isolates (8.24%) were vancomycin resistant (VRSA) and 18 S. aureus isolates (18.5%) resist tetracycline antibiotic. Sixty four CONS isolates (62.13%) were methicillin resistant, 28 CONS isolates (27.18%) resist tetracycline, and 5 CONS isolates (4.85%) were vancomycin resistant. Suckling mouse bioassay was tested to investigate the staphylococcal enterotoxin biological activity. Results showed that 131 isolates which constitutes 65.5% of the examined isolates, gave a positive result. Both COPS and CONS isolates were shown to be enterotoxigenic, COPS represented by S. aureus species occupied the higher ratio of the enterotoxigenic staphylococci, 86 S.aureus isolates (65.64% of the enterotoxigenic staphylococci) gave the positive ratio of the intestine weight to the body weight which was ≥ 0.083 while 45 CONS isolates (34.35%) were enterotoxin producers. These toxins were thermostable staphylococcal enterotoxins which gave the same toxic effect after heating to 100°C for 30 minutes.
The effects of essential oilNigella sativa and Menthawas study on the chemical, microbial and sensory properties for soft white cheese that produced from it during storage at 0, 7 and 14 days .The results show significantly percent decrease in moisture for all samplesand maximum decrease was at the latest storage period for all them .The reduced in moisture was accompanied with increase in percentage of protein and fat during of storage period for all samples.
The control sample showed increased in bacterial logarithmic for total count bacterial, coliform, Staphylococcus aureus, proteolytic bacteria, lipolytic bacteria and mold and yeasts during of storage period , the highest results showed at the latest storage period 14days, it w
Chlorination has been the method of choice for disinfecting water used for drinking purposes. However, some stressed bacteria during chlorination are able to recover and alter the potability of water. This study assessed the recovery of stressed bacteria in dechlorinated water. Ten chlorinated water samples were collected from different points within Ilorin metropolis, Kwara, Nigeria. The samples (100ml) were dechlorinated with 0.1ml of 11.4mM sodium thiosulphate solution. The physicochemical characteristics of the chlorinated water samples were determined while bacteriological analyses were carried out on both chlorinated and dechlorinated water samples. The antibiotic susceptibility pattern of the isolates was determined using disc dif
... Show MoreRaw milk is subjected to various physical, chemical and biological pollutants due to produce, a transporting and marketing processes. The current study was designed to examine raw milk samples which were collected from different locations of Iraq. The highest mean value of Pb content [1.801±0.311 ppm] was recorded while the lowest mean value [0.941±0.104 ppm]. The highest mean value [1.532±0.124 ppm] and gave the lowest mean value [0.063±0.044 ppm] of Cd content. This study has the highest mean value of Cu content was [0.931±0.092 ppm] and but the lowest mean value was [0.308±0.029 ppm]. Metals contents of raw milk in this study showed higher than standards of the International Dairy Federation [IDF].
This study was aime to investigate the effect of addition different concentration of celery leaves to white soft cheese ,Treated cheese between 2018-2019, ,The finely Celery (Apium graveolens) leaves were adding to crude white cheese after texturizing in three leveles included (A,B,C) in addition of control antimicrobial activity of celery treated cheese against total account bacteria and coliform bacteria was estimated during (0, 5, 10, 15, 20) days. The results were shown that the higher concentration of celery in treated cheese, had a lower concentration of protein, lipid and ash content ( 16.81,15.13 and 4.30% respectively, but it had a higher moisture content 59.50%.also the total bacteria counts were decreasing significantly (0.05 P)w
... Show MoreStaphylococcus Sp.is the most common type of bacteria found in contamination place, we design this
study to compare the contamination accident between two hospitals in Baghdad.One of them isthe Burns
Specialist Hospital in the Medical CityinRusafa and another one is Al-Karama Hospital in Karkh. The
samples were collected fromOperativeWard No1 (OW1), Operative Ward No2 (OW2), Consulting Pharmacy
(CP), Emergency Room (ER), Reception Room (RR), Women's Ward (WW) and Men's Ward (MW).The
samples were taken from inside each clinical unit, surfaces, food, and air. The results showed that the
number of samples containing Staphylococcus sp. bacteria is 81, including 45 belonging to Al-Karama Burns
Ward Ho
Bodies of water are usually being polluted by wastes from domestic and industrial sources thereby making them unfit for use. Hence, this study aimed at assessing the water quality from Asa River, Ilorin, Nigeria in terms of bacteriological and physicochemical parameters. The bacteriological parameters assessed were heterotrophic bacterial count, total coliform, faecal coliform, identification of the isolates, antibiotic resistance patterns, and plasmid profile of the isolates. Whereas, the assessed physicochemical parameters were pH, total chloride, suspended solid, and total hardness. The heterotrophic bacterial count, total coliform, and faecal coliform counts ranged from 7.6 x 103 to 3.2 x 106 cfu/ml,
... Show MoreThis study was designed to evaluate the effects of cellulose membranes produced by Acetobacter xylinum bacteria, after enrichment of the growth media with Alzahdy palm dates syrup to enhance cellulose production for reducing the contamination of locally-produced white cheese with pathogenic bacteria. Cellulose was vitally activated by incubation with both probiotics Lactobacillus acidophilus and Lactobacillus plantarum and the effectiveness of the produced cellulose membranes was measured by studying six characteristics: elongation, tensile strength, membrane rupture, permeability to oxygen, permeability to water vapor, and thickness (mm). The produced membranes showed remarkable functionality and characteristi
... Show MoreBackground: Staphylococcus aureus infections are growing problems worldwide with important implications in hospitals. The organism is normally present in the nasal vestibule of about 35% apparently healthy individuals and its carriage varies between different ethnic and age groups.
Objective:To study the antibiotic resistance of staphylococcus aureus isolated from nasal cavity of Health Care Personnel.
Patients and methods: A total of 180 samples were collected from the nose of the two groups (health care personnel, community control) at Baghdad Teaching Hospital. They were screened for nasal colonization with S.aureus during the period between Apr
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