Aeromonas hydrophila is widely distributed throughout the world and causes diseases to animals and human exposed to contaminated environments such as water and soil. This study aimed to compare between isolates of A. hydrophila collected from clinical and environmental samples, through investigating the phenotype of some virulence factors in vitro, including hemolysin, protease, lipase, nuclease and biofilm formation ability. Also, the antimicrobial susceptibility for different antibiotics was determined using disc diffusion method. For genotypic identification of isolates and phylogenetic tree construction, 16S rDNA target gene was amplified and sequenced. The phenotypic results showed some differences between the isolates (clinical and environmental). All isolates were resistance to clindamycin, amoxicillin and erythromycin while susceptible to gentamicin, amikacin and vancomycin. Sequences of 16S rDNA confirmed the identification of the studied bacteria as A. hydrophila with 99-100% , and identity and phylogenetic tree by neighbor-joining clearly separated the isolates in a branching pattern which displayed similarity to the GenBank isolates obtained from Asian regions. The clinical isolates showed less polymorphism than the environmental isolates.
From 50 stool samples collected from children with diarrhea of both sexes who visited various hospitals in Baghdad, 26 isolates of E.coli were found to belong to the phylogenetic group E. The findings revealed that the percentage of E.coli for thephylogenetic group E is (52%) , making it the dominant group among the other phylogenetic groups. The findings demonstrated that 100% of the E.coli isolates from phylogenetic group E are resistant to penicillin, and only 15% are resistant to imipenem. Multi-drug resistance (MDR) was found to be 15%, while XDR reached 85%. The results of thephylogenetic group for the remaining species of isolates in this study were group A (2/50 and by 4%), gr
... Show MoreObjective of the study was to rate and analyze the physical fitness of female students from Iraq and Russia to find similarities, differences and regularities. Sampled for the study were 44 female non-sporting students: 20 students from Pedagogical University in Bagdad; and 24 students from Russian State University of Physical Education, Sport, Youth and Tourism in Moscow. The study was designed to obtain the joints flexibility, coordination, speed-strength and static endurance rates. The Iraqi students were tested with higher coordination abilities than their Russian peers, whilst the latter were ranked higher in the speed-strength, shoulder joints and spine flexibility tests, albeit lower than their Iraqi peers in the hip flexibility test
... Show MoreTwo orders of zooblankton,were studied Cladocera and Copepeda in two classes Calanoida and Cyclopoda, where it was studied inside floating cages and for breeding fish placed in the Tigris River in the Rashidiya area. Has been study The population density of zooplankton groups and measure some chemical and physical characters, was studie where she collected samples of zooplankton and water from two locations of cages (inside the cages, after 100m from cages). The study was conducted within six months from January to the end of June 2014 during which there was study of the Wallace pH, water temperature, biological oxygen demand and dissolved oxygen, as to the zooplankton study the species disappeared inside cages of fish and existed after
... Show MoreThe present study was conducted to reveal the effect of crude oil on some fungal species isolation from soil in order to evaluate the role of these fungi in environmental balance of soil . The results showed a variation in numbers and percentage of the fungal isolates Aspergillus fumigatus dominated over all isolates with a frequency of (32.47) . In respect of the effect of different concentrations of the crude oil, low concentrations (0.05, 0.1) % showed no effect on radial growth ( mean colony diameter) of the isolated fungi grown &nbs
... Show MoreAntibiotics are essential for treating infectious diseases, but their overuse and adverse effects are raising concerns about global public health. The pervasiveness of antibiotic contamination in aquatic environments has drawn increased attention in recent years. The primary concern regarding the release of antibiotics into the environment is the potential for microorganisms to become resistant to antibiotics. This review article summarizes the analytical methods used to determine the presence of trimethoprim and metronidazole in various environmental samples. These antibiotics have traditionally been analyzed using tandem mass spectrometry or high-performance liquid chromatography coupled to mass spectrometry; fluorescence or ultrav
... Show MoreOne of the most important problems confronts hospitals is the strains emergence of Enterococcus spp. with multiple resistance to antibiotics, which propel researchers to modify or produce new antibiotics or combination between two antibiotics so that to be more effective against Enterococcus . This study was aimed to susceptibility some of local Enterococcus spp. Isolates with of 21 antibiotic using disc diffusion method. The results showed absolute resistant 100% toward (Cephalexin , Gentamycin , Amikacin ,Erythromycin and Nalidixic acid), while showed a high sensitivity toward (Vancomycin and Impenem ) at percentage of 92.3% for each . Also highl
... Show MorePseudomonas aeruginosa is the most common opportunistic pathogen causing morbidity and mortality in hospitalized patients due to its multiple resistance mechanisms. Therefore, as a therapeutic option becomes restricted, the search for a new agent is a preference. So P. aeruginosa is an extremely versatile Gram-negative bacterium capable of thriving in a broad spectrum of environments, and this performs main problems to workers in the field of health. One hundred and fifty samples were collected from different sources from Baghdad hospitals, divided into two main groups: clinical (100) specimens and (50) samples as an environmental, collected from October 2019 to the March 2020. All of these samples were cultured by specific and differential
... Show MoreThe measurements of major and trace elements in different brands of milk powder selected from the Iraqis market via the X-ray fluorescence (XRF) Technique have been studied in the present work. The result of the measurements reveals the high concentrations of sodium, phosphorus, sulfur, chlorine, potassium, calcium and magnesium. Furthermore, low concentrations of aluminum, silicon, iron, bromine, molybdenum, iodine, barium, titanium, manganese, cobalt, chrome, nickel, copper, zinc and lead were detected. Neutron activation analysis (NAA) and Kjeldahl technique were also employed to determine the concentrations of nitrogen. It was found that the nitrogen concentration was in the range of (1.96 - 3.23) % which is within the permissible li
... Show MoreThe genus Bacillus bacterium isolated from soil and tests their ability to produce glutamic acid, the bacteria are diagnosed by traditional methods and VITEK-2 system. And we studied the optimal conditions for production and try to improve the productivity of the isolation by manipulating the components of the production medium and of the circumstances environmental of production medium. There is 43 isolates of the 70 bacterial isolates belonging to the genus Bacillus depending on phenotypic characteristics of the colonies and microscopic characteristics and found that 13 isolated revert to species B.subtilis depending on physiological and biochemical characteristics. As well as on microscopic and biochemical tests, 13 isolates were subj
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