Red pigmented undecylprodigiosin produced by Streptomyces coelicolor (A3)2 is a
promising drug owing to its characteristics of antibacterial, antifungal,
immunosuppressive and anticancer activities. The culture of S. coelicolor in liquid
medium produces mainly the blue pigmented actinorhodin and only low quantities of
undecylprodigiosin. From an industrial point of view, it is necessary to find a strategy to
improve undecylprodigiosin production. The present study provides evidence that
cultivation of S. coelicolor on solid substrate resulted in a reversal in this pattern of
antibiotic production as the production of undecylprodigiosin was significantly increased
and actinorhodin was completely suppressed. Four different solid substrate (wheat bran,
soya bean ground, rice husk and ground corn) were tested for their ability to support the
maximal production of undecylprodigiosin in solid state fermentation. Wheat bran
showed the highest production of undecylprodigiosin, starting from the first day of
incubation at a moisture level of (1:1 weight: volume) and reaching its maximum of 16
mg/gds on the fourth day. In addition, we report the exploitation of the interspecies
interaction in order to enhance undecylprodigiosin production by introducing live or
dead cells of E. coli, Bacillus subtilis and Saccharomyces cerevisiae, separately, to
Streptomyces coelicolor solid substrate fermentation. Our results revealed a significant
increase in the production of undecylprodigiosin in the elicited cultures compared with
control. The maximum enhancement occurred in the culture elicited with the live cells of
B. subtilis with an increase of 2-fold compared with control.
Mixed ligand complexes of Cu(II), Ni(II) and Co(II) with metformin(MTF) as primary ligand and cysteine(Cys) as secondary ligand have been prepared and characterized by elemental analysis, atomic absorption, molar conductivity, magnetic susceptibility measurements, FTIR,UV-Vis ,1H-NMR and 13C-NMR spectral studies. The elemental analysis, atomic absorption data reveal the formation of [1:1:1] [M:MTF:Cys] complexes.The electronic spectra and magnetic moment measurements reveal the presence of complexes in an octahedral geometry and the molar conductivity studies of the complexes indicate their non-electrolytic nature. The infrared and NMR spectral were showed that the chelation behaviour of the ligands towards selected transition metal ions
... Show MoreThe inelastic C2 form factors and the charge density distribution (CDD) for 58,60,62Ni and 64,66,68Zn nuclei has been investigated by employing the Skyrme-Hartree-Fock method with (Sk35-Skzs*) parametrization. The inelastic C2 form factor is calculated by using the shape of Tassie and Bohr-Mottelson models with appropriate proton and neutron effective charges to account for the core-polarization effects contribution. The comparison of the predicted theoretical values was conducted with the available measured data for C2 and CDD form factors and showed very good agreement.
A design for a photovoltaic-thermal (PVT) assembly with a water-cooled heat sink was planned, constructed, and experimentally evaluated in the climatic conditions of the southern region of Iraq during the summertime. The water-cooled heat sink was applied to thermally manage the PV cells, in order to boost the electrical output of the PVT system. A set of temperature sensors was installed to monitor the water intake, exit, and cell temperatures. The climatic parameters including the wind velocity, atmospheric pressure, and solar irradiation were also monitored on a daily basis. The effects of solar irradiation on the average PV temperature, electrical power, and overall electrical-thermal efficiency were investigated. The findings i
... Show MoreBackground: Researchers have found that interleukin 6 (IL-6) plays a crucial regulatory function in the onset and progression of a wide range of inflammatory disorders. One of the more prevalent inflammatory illnesses affecting people today is rheumatoid arthritis.
Aim of the study: The purpose of this study was to compare the IL-6 levels of rheumatoid arthritis (RA) patients to those of healthy controls and to examine the relationship between IL-6 and RA-related demographic and clinical factors.
... Show MoreBackground: Radiation therapy has the ability to destroy healthy cells in addition to cancer cells in the area being treated. However, when radiation combines with doxorubicin, it becomes more effective on breast cancer treatment. Objective: This study aims to clarify the effect of X-ray from LINAC combined with amygdalin and doxorubicin on breast cancer treatment, and the possibility of using amygdalin with X-ray instead of doxorubicin for the breast cancer treatment. Method: Two cell lines were used in this study, the first one was MCF-7 cell line and second one was WRL- 68 normal cell line. These cells were preserved in liquid nitrogen, prepared, developed and tested in the (place). The effect of three x-ray doses combined with a
... Show MoreThree new polyphosphates were synthesized in good yields by reacting diethylenetriamine with the appropriate phosphate ester in ethanol under acidic conditions. The polyphosphate structures were determined using FT-IR and 1H-NMR spectroscopies, and their elemental compositions were confirmed by EDX spectroscopy. Polyphosphates were added to poly(vinyl chloride) (PVC) at low concentrations to fabricate thin films. The PVC films were irradiated with ultraviolet light for long periods, and the effect of polyphosphates as the photostabilizer was investigated by determining changes in the infrared spectra (intensity of specific functional group peaks), reduction in molecular weight, weight loss, and surface morphology. Minimal changes we
... Show MoreObjectives: To identify the frequency and types of microsatellite instability among a group of sporadic CRC patients and to correlate the findings with clinicopathological characteristics. Methods: During an 8-month period, all patients with sporadic CRC who attended to two teaching hospitals in Baghdad, Iraq were recruited to this cross-sectional study regardless of age, sex, ethnicity, or tumor characteristics. Demographic, clinical, and histopathological features were recorded. DNA was extracted from FFPE-blocks of the resected tumors and normal tissues. PCR amplification of five microsatellite mononucleotide repeat loci (BAT25, BAT26, NR-21, NR-24, and MONO-27) and 2 pentanucleotide repeat control markers (Penta C and Pent
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