This study reflected on the relationship between contactin associated protein-like
2 gene (CNTNAP2) and autism spectrum disorders. The study includes forty autistic
patients and forty non autistic children as control groups (twenty unaffected sibling
and twenty unrelated children). DNA was extracted from Blood samples for
molecular detection of CNTNAP2 mutations associated with ASDs by using
Polymerase Chain Reaction (PCR) technique and sequencing analysis. PCR reaction
was performed to amplify exon 20 of CNTNAP2 gene. The PCR results revealed that
identical bands related to exon 20 of CNTNAP2 gene were present in all samples.
Therefore, five samples (four from autistic patients and one from control sibling)
were selected for genotype analysis of CNTNAP2 (exon 20) by direct sequencing.
Genotype analysis revealed that there were no any variants in CNTNAP2 (exon 20),
but it shows that two different mutations were identified in non coding region
(introns) of the CNTNAP2 gene (Single Nucleotide Polymorphisms (SNPs),ID SNP:
rs3779032 A/G in 2118282 position and ID SNP: rs3779031 A/C in 2118436
position). rs3779031 A/C are located at intron 19 while SNP rs3779032 A/G are
located at intron 21. These mutations were seen only in autistic patients but not
present in control sample. The current study showed that two common SNPs
(rs3779031 and rs3779032) in CNTNAP2 were strongly associated with ASDs,
where the frequencies of these SNPs were relatively high. SNP rs3779032 were
identified in two autistic patients while rs3779031 were identified in three autistic
patients from four unrelated families with ASDs.
There are growing concerns over the possibility of transfer genetically modified
sequences from genetically modified feed component (GM feed) to animals and
their products, moreover, affect these sequences on animal and human health. This
study was implemented to detect P35S in modified feed by using PCR technique by
detecting presence P35S promoter, which responsible for the regulation of gene
expression for most of the transgenic genes. Thirty eight feed samples were
collected from different sources of Baghdad markets, which have been used for
feeding livestock, comprise 21 coarse mixes feed, 13 pelleted feed, and 4 expanded
feed. Genomic DNA was extracted by using two methods, CTAB method and
Wizard kit. In
There are growing concerns over the possibility of transfer genetically modified sequences from genetically modified feed component (GM feed) to animals and their products, moreover, affect these sequences on animal and human health. This study was implemented to detect P35S in modified feed by using PCR technique by detecting presence P35S promoter, which responsible for the regulation of gene expression for most of the transgenic genes. Thirty eight feed samples were collected from different sources of Baghdad markets, which have been used for feeding livestock, comprise 21 coarse mixes feed, 13 pelleted feed, and 4 expanded feed. Genomic DNA was extracted by using two methods, CTAB method and Wizard kit. In order to verify the presenc
... Show MoreThe present study aims to detect CTX-M-type ESBL from Escherichia coli clinical isolates and to analyze their antibotic susceptibility patterns. One hundred of E. coli isolates were collected from different clinical samples from a tertiary hospital. ESBL positivity was determined by the disk diffusion method. PCR used for amplification of CTX-M-type ESBL produced by E. coli. Out of 100 E. coli isolates, twenty-four isolates (24%) were ESBL-producers. E. coli isolated from pus was the most frequent clinical specimen that produced ESBL (41.66%) followed by urine (34.21%), respiratory (22.23%), and blood (19.05%). After PCR amplification of these 24 isolates, 10 (41.66%) isolates were found to possess CTX-M genes. The CTX-M type ESBL
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The development taking place on the sports field at the general level and in the game of football at the private level, being the most popular game in the world, has reached very advanced stages. The use of modern aids and technology has contributed greatly to this development from the collective and individual aspects, as the goalkeeper is the most important position in the team, the use of devices gave objective readings about the goalkeeper’s ability in terms of skill and physical aspects, as the importance of the research lies in designing an electronic device to measure the degree of bending of the knee angle because of its great importance in developing the skill of catching and dimensions of the high ball for football goalkeepers.
... Show MoreHedging is a linguistic avoidance of full commitment or precision. It is the use of a vague language. The main objectives of this study are to
... Show MoreLas diferencias entre el pretérito perfecto simple y el imperfecto son uno de los temas más complejos de la lengua española, no sólo para el estudiante, sino para el profesor, puesto que sistematizar los casos y hacerlos fácilmente comprensibles al alumno es tarea difícil. Los profesores de lengua, cuyas investigaciones han dado lugar a una serie de teorías y de corrientes metodológicas y didácticas que permiten enfocar la enseñanza de una lengua extranjera de manera muy distinta a como se hacía tradicionalmente. Vamos a repasar muy brevemente cuáles son estas teorías.
En primer lugar es necesario señalar en qué consiste el aprendizaje de una segunda lengua:
"El aprendizaje de una segunda lengua (L2) es el
... Show MoreThis research seeks to study the role of proactive leadership as an essential element that helps all federations that lead the wheel of sports, including the Iraqi Handball Federation, so that it builds a correct environment that helps manage the organizational errors that the Handball Federation may fall into, and this in turn helps in early detection of errors and obstacles that may occur. It is likely that the Federation will fall into the process of managing and organizing the Iraqi Handball League, in addition to increasing the clubs’ ability to assist the Iraqi Handball Federation by being proactive so as not to make mistakes. The research community included the administrative bodies of the clubs participating in the Iraqi E
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The research aims to show the relationship between the use of automated accounting systems technology and its impact on enhancing the efficiency and effectiveness of the internal control system in a sample of Bahraini universities in light of the rapid changes in the electronic business environment. Automated accounting and its impact on enhancing the efficiency and effectiveness of the internal control system, and it is concluded through the analytical study of the research sample that there is a percenta
... Show MoreThe purpose of this study was to find out the connection between the water parameters that were examined in the laboratory and the water index acquired from the examination of the satellite image of the study area. This was accomplished by analysing the Landsat-8 satellite picture results as well as the geographic information system (GIS). The primary goal of this study is to develop a model for the chemical and physical characteristics of the Al-Abbasia River in Al-Najaf Al-Ashraf Governorate. The water parameters employed in this investigation are as follows: (PH, EC, TDS, TSS, Na, Mg, K, SO4, Cl, and NO3). To collect the samples, ten sampling locations were identified, and the satellite image was obtained on the
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This research is an attempt to develop exercise with weights to strengthen some of the striking muscles in the shoulder and arm and to develop the accuracy of the smash and rectum skills. The importance of this paper lies in the study of moments of force to achieve the ability to control muscular work and to explore the impact of physical and skill exercises with weights to develop moments of force for some muscles. The experimental method on a sample of players, selected according to the intentional method, including ( 9) advanced players representing Air Force Club participating in the Premier League for season 2011-2012. It is concluded that the exercises proposed have their effective impact on developing the variables of moments force f
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