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Detection of antibiotics resistance genes in clinical isolates of Klebsiella pneumonia

Total of 46 isolates of Klebsiella pneumoniae were collected from patients attending (Al-Yarmook Hospital and Education Labs / medical city), and isolates were re-identified, depending on morphology and biochemical tests . Disk diffusion method was employed to determine antibiotic susceptibility of forty six isolates by using eleven antibiotics .The results revealed the sensitivity of six isolates (9.3%) to Imipenem and Meropenem . On the other hand the isolates were showed 23.9% resistant against Ciprofloxacin, while some isolates shown higher resistant against several antimicrobial agents such as 65.2%, 69.0% for Amikacin and Cefepime consequently , 71.1%, 71.7 % for Amoxicillin -Clauvulanic acid and Gentamicin and 82.6% against Piperacillin , Nitrofurantoin and Ceftazidime. The isolates also appeared high level of resistance against Cefotaxime at a percentage 91.3% . Depending on the obtained results 6 isolates were selected assigned (K21, K32, K33, K37, K38, K43) for detection of blaTEM, blaSHV, blaKPC and AmpC because of its resistance of almost chosen antibiotics. The selected isolates were PCR-positive for blaTEM which showed bands in 209 pb., on the other hand the result revealed that the isolates K33,K32 posses encoding to blaSHV of 590pb in size,. In regard to blaKPC gene only K37 (16%)gave 811 pb . All selected isolate gave negative results to AmpC. The selected isolates were detected of beta-lactamase production by using acidimetric tests (tube method) , all isolates gave positive result.

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Publication Date
Tue Nov 19 2024
Journal Name
Journal Of Baghdad College Of Dentistry
Awareness and knowledge of oral cancer among final year undergraduate dental students in Baghdad-Iraq

Background: The incidence of oral cancers is increasing all over the world. Early detection ofthis important public health matter makes them more amenable to treatment and allows the greatest chance of cure.The aim of this study was to investigate the awareness and knowledge on oral cancer among final -year dental students in Iraq. Materials and methods: Questionnaires were delivered to 160 final–year dental students in the College of Dentistry in Baghdad. The questionnaire focused on the awareness/knowledge of oral cancer, earlyand common clinical signs and symptoms andassociated risk factors. Results: It was found that 87% of students were aware of oral cancer. The followings were recognized as signs and symptoms of oral cancer: persis

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Publication Date
Mon Dec 24 2018
Journal Name
Bulletin Of The Iraq Natural History Museum (p-issn: 1017-8678 , E-issn: 2311-9799)
MOLECULAR IDENTIFICATION AND PHYLOGENETIC-TREE ANALYSIS OF MONIEZIA SPECIES FROM SHEEP IN AL-DIWANIYAH CITY

    The present study was performed to detect the molecular and the phylogenetic identification of species that belonging to the genus of Moniezia Blanchard, 1891 which affected intestines of sheep in Al-Diwaniyah city, Iraq; fifty intestine samples were sought for the infestation of Moniezia spp. from the city slaughterhouse from 1 October to 30 November 2017, this tapeworm was found to infest the intestines of 13 sheep.

 

    For morphological identify the genus of this tapeworm, eggs from one gravid proglottid of the thirteen worms were examined, polymerase chain reaction (PCR) and the PCR-product-based sequencing were applied on 4 Moniezia tapeworms targeti

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Publication Date
Sun Apr 29 2018
Journal Name
Iraqi Journal Of Science
Estimation the Missing Data of Meteorological Variables In Different Iraqi Cities By using ARIMA Model

In this paper, ARIMA model was used for Estimating the missing data(air temperature, relative humidity, wind speed) for mean monthly variables in different time series at three stations (Sinjar, Baghdad , AL.Hai) which represented different parts of Iraq from north to south respectively

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Publication Date
Sun Apr 23 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Differential Screening of Randomly Amplified cDNAs Using RAPD Primers in Salt Tolerance and Sensitive Wheat

 The identification of salinity-tolerance genes is a critical aspect of the new molecular technology. In this work cDNA-RAPD is used for the identification of genes expressed in salt tolerant but not in salt sensitive wheat. Two cultivars wheat, salt tolerance (Dijla) and sensitive (Tamooz2) were used for the preparation of RNA and cDNA synthesis. Eight primers were used for random amplification of cDNA constructed from RNA and three primers were differentially expressed in salt tolerant cultivars. Genes related to salt tolerant were predicted using NCBI blast for the three primers. The predicted genes were involved in salt tolerance of wheat and other plants as well. This indicates the suitability of the primers and the method for

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Publication Date
Sun Dec 07 2014
Journal Name
Baghdad Science Journal
Estimation of Serum Osteocalcin Levels in Osteoporotic Postmenopausal Iraqi Women with Type 2 Diabetes Mellitus

Background: Osteoporosis (OP) is a systemic disease characterized by low bone mass and micro architectural deterioration of bone tissue, resulting in an increased risk of fractures and has touched rampant proportions. Osteocalcin, one of the osteoblast-specific proteins, showed that its functions as a hormone improves glucose metabolism and reduces fat mass ratio. This study is aimed to estimate the osteocalcin and glucose level in blood serum of osteoporotic postmenopausal Women with and without Type 2 Diabetes.Materials and methods: 60 postmenopausal women with osteoporosis divided into two groups depending on with or without T2DM, 30 patients for each. Serum samples of 30 healthy postmenopausal women were collected as control group. Ost

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Publication Date
Thu Aug 03 2023
Journal Name
Journal Of Legal Sciences
Recruitment of Persons in Terrorist Organizations, his Techniques, his Methods - UAE Law as a Model

This study aims to investigate the nature of the recruitment of people into terrorist organizations that have spread in international societies, this phenomenon that did not arise out of a vacuum, but rather that it has its causes and its intellectual, psychological, political, and social motives, as well as economic and educational motives. These reasons may be intertwined with each other, so today we are facing an important and dangerous issue launched by terrorist organizations, by recruiting certain people, who are carefully selected and according to specific specifications, taking advantage of their social and family problems, the class differences they suffer from in their societies, and their desire for revenge, for their feelings

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Publication Date
Thu Aug 03 2023
Journal Name
Journal Of Legal Sciences
Evidence of Proof in the Administrative Investigation and Employee Guarantees Towards it : A Comparative Study

The aim of this research is to identify the guarantees of collecting evidence in the administrative investigation in both the Federal Decree-Law No. (11) of 2008 regarding human resources in the federal government and its amendments, and Ministerial Resolution No.(1) of 2018 regarding the executive regulations of the Human Resources Law in the Federal Government, Compared to the Jordanian Civil Service System No.(9) for the year 2020. In order to find out the guarantees obtained by the employee to whom the job violation is attributed in order to confront the authority of the administration in the event that he is referred to the administrative investigation in the stage of collecting evidence. And to search for the balance between the le

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Publication Date
Tue Nov 06 2018
Journal Name
Iraqi National Journal Of Nursing Specialties
Assessment of Nurses' Knowledge toward Children with GuillainBarre Syndrome at Pediatric Hospitals in Baghdad City

Objectives: The study aimed to assess the level of pediatric nurses' knowledge toward children with Guillain-Barre
syndrome (GBS) and to find out the relationships between nurses' knowledge and their demographic data.
Methodology: A purposive “non probability” sample of (30) nurses was selected from medical neurological wards and
Respiratory Care Units of Children Welfare Pediatric Teaching Hospital, Child’s Center Pediatric Teaching Hospital,
AL-Kadhemia Teaching Hospital, Baghdad Teaching Hospital, and Neurological Science Hospital which has started
from March 5
th 2009 to April 30th 2009. The study instrument consists of two parts. The first part is concerned with
nurses' demographical characteristic and the

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Publication Date
Tue Oct 01 2013
Journal Name
Journal Of Economics And Administrative Sciences
Determinants of economic growth in Arab Countries: An Empirical Study compared with South-east Asia

The study addresses the problem of stagnation and declining economic growth rates in Arab countries since the eighties till today after the progress made by these countries in the sixties of the last century. The study reviews the e

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Publication Date
Sun Jun 11 2017
Journal Name
Al-academy
The approach of deliberation in the ontemporary Iraqi theater text - theatricality Afwah-as a odel

The deliberative system of communicative studies is exposed to cultural openness, according to modern cultural studies, to establish a verbal language system that achieves reciprocal and cross-cultural relations. This research has examined the concept of deliberative approach in the contemporary Iraqi theater text by studying the deliberative system of text and its interrelationship with The stage of the verbal embodiment of the dramatic event, because the dramatic text achieves in its construction of racism, a deliberative approach between the verbal event and the language, and what later leads to the completion of the dramatic action. The research included four chapters. The first chapter was the systematic framework of the research, T

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