Dictyostelium discoideum amoeba is a powerful system to study gene function through genetic and functional analysis. Paracaspase (Pcp) is a caspase-like protein that has recently identified in D. discoideum. Study paracaspase molecular function in Dictyostelium will provide insight into its cellular role in more complex organisms and the possibility to use it as a drug target against the parasitic amoeba. In this study, pcp was tagged with Green Fluorescent Protein (GFP) and over-expressed in the cell. The knockout version of pcp (pcp-) was also investigated. In the course of our examination, it was observed that cells overexpress Pcp was unable to complete the developmental process, leading us to examine its role in development. Those cells were unable to initiate early development and failed to aggregate under starvation conditions. Aggregation is severely defected as cells have decreased cell-cell cohesion. In particular, these cells demonstrated a reduction in adhesion. This data suggests that over-expression of Pcp causes a signaling defects crucial to normal development in Dictyostelium. On the other hand, cells with pcp- mutant demonstrated larger aggregation in normal media as well as under starvation conditions compared to the control cell lines. As a result, we propose that Dictyostelium Pcp is a regulated protein involved in cellular functions including signal relay pathways that are essential for cell aggregation and development. Thus, Pcp protein is a candidate drug target against the amoebic parasitic infection by prevent development to their infective stages.
A simple physical technique was used in this study to create stable and cost-effective copper oxide (CuO) nanoparticles from pure copper metal using the pulsed laser ablation technique. The synthesis of crystalline CuO nanoparticles was confirmed by various analytical techniques such as particle concentration measurement using atomic absorption spectrometry (AAS), field emission scanning electron microscopy (FE-SEM), the energy dispersive X-ray (EDX), and X-ray diffraction (XRD) to determine the crystal size and identify of the crystal structure of the prepared particles. The main characteristic diffraction peaks of the three samples were consistent. The corresponding 2θ is also consistent, and the cytotoxicity of the nanoparticles was
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Data centric techniques, like data aggregation via modified algorithm based on fuzzy clustering algorithm with voronoi diagram which is called modified Voronoi Fuzzy Clustering Algorithm (VFCA) is presented in this paper. In the modified algorithm, the sensed area divided into number of voronoi cells by applying voronoi diagram, these cells are clustered by a fuzzy C-means method (FCM) to reduce the transmission distance. Then an appropriate cluster head (CH) for each cluster is elected. Three parameters are used for this election process, the energy, distance between CH and its neighbor sensors and packet loss values. Furthermore, data aggregation is employed in each CH to reduce the amount of data transmission which le
... Show MoreBackground: Asthma is an inflammatory airway disease; this inflammatory response can be attributed to reduced lymphocyte apoptosis in peripheral blood and in airway tissues.
The mechanism behind this could be attributed to decreased Bcl2 protein and increase Bax protein in peripheral blood lymphocytes of asthmatic patients.
Aim: to explore the mechanism behind decreased lymphocyte apoptosis in peripheral blood of asthmatic patients at cellular level.
Method: Ninety four subjects; (44) control and (50) patients were included in this study during the period from (2003) to (2004). The aspirated lymphocytes for each individual
were prepared and stained by immunocytochemistry to study the percentage of antiapop
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Background: Oral SCC is a complex malignancy where environmental factors, viral infections and genetic alterations most likely interact, and thus give rise to the malignant condition. The HSP70 play a direct role in apoptosis inhibition by aligning the improved integrity of a cell’s proteins with the improved chances of that particular cell’s survival.P21 gene produces p21 protein which is a potent cyclin-dependent kinase inhibitor that plays a significant role in carcinogenesis. The aims of the study were to evaluate and compare the immun-histochemical expression of the HSP70 and cell cycle protein p21in NOM, OED, and OSCC. Correlate both marker expressions with each other. Materials and methods: Forty six formalin-fixed, par
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Due to the significant role in understanding cellular processes, the decomposition of Protein-Protein Interaction (PPI) networks into essential building blocks, or complexes, has received much attention for functional bioinformatics research in recent years. One of the well-known bi-clustering descriptors for identifying communities and complexes in complex networks, such as PPI networks, is modularity function. The contribution of this paper is to introduce heuristic optimization models that can collaborate with the modularity function to improve its detection ability. The definitions of the formulated heuristics are based on nodes and different levels of their neighbor properties. The modulari
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Carrageenan extract is a compound of sulfated polyglycan that is taken out from red seaweeds. Being hydrocolloid in nature, carrageenan has gelling, emulsifying and thickening properties allowing it to be commonly used in the oral healthcare products and cosmetics. Due to its bioactive compounds, carrageenan has been shown to have antimicrobial, antiviral, and antitumor properties. The purpose of this work is to study the probable use of carrageenan on the diseases that are related to oral cavity and on the genomic DNA in in vitro experimental model
In this study, the effects of k-carrageenan on four different cell lines related to the cancer and normal cells which cultured on selective media were done. Moreover, the eff
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Nanofluid treatment of oil reservoirs is being developed to enhance oil recovery and increase residual trapping capacities of CO2 at the reservoir scale. Recent studies have demonstrated good potential for silica nanoparticles for enhanced oil recovery (EOR) at ambient conditions. Nanofluid composition and exposure time have shown significant effects on the efficiency of EOR. However, there is a serious lack of information regarding the influence of temperature on nanofluid performance; thus the effects of temperature, exposure time and particle size on wettability alteration of oil-wet calcite surface were comprehensively investigated; moreover, the stability of the nanofluids was examined. We found that nanofluid treatment is more efficie
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