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Genetic Comparative Study of Staphyolococcus aureus& Staphylococcus epidermidis Isolated from Wounds, Burns and Skin Flora

One hundred specimens from wounds, burns, and skin swabs were collected
from patients laying and attended to Balad general hospital. It was found that 50
isolates belong to Staphylococcus spp., 38 isolates were identified as S. aureus and
12 isolates were identified as S. epidermidis according to microscopic, cultural and
biochemical testing. The study of seven extracellular enzyme as virulence factors
including the enzymes: urease, lipase, DNase, haemolysin, coagulase, β-lactamase,and lecithinase. Reavealed that 100% of S.aureus had the ability to produce these
enzymes, while S. epidermidis isolates were unable to produce the enzymes DNase,
lipase, coagulase, but they were capable to produce haemolysin, urease, lecithinase,
and β-lactamase, the range for production of these factors were 58.33 %,80,%, 10,%,
and 45% respectively.
Eighteen Staphyolococcus isolates were selected according of their ability for
production most of studies virulence enzymes for detection of genes encoding for
the enzymes heamolysine (hly) and coagulase (coa) by using of polymerase chain
reaction (PCR) technique. The Gene hIy was detected in all isolates and coa was
detected in 14 isolates only.

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Publication Date
Sun Apr 30 2023
Journal Name
Iraqi Journal Of Science
Molecular Characteristics of Multidrug Resistant Acinetobacter baumannii Isolated from Baghdad Hospitals

A total of 100 clinical sample from (urine, sputum and swabs of wound , burn and ear) were collected from patients in different hospitals of Baghdad during the period from December 2013 to May 2014. 15 isolates (15%) identified belong to Acinetobacter baumannii, swabs of wounds were represented in high percentage of A.baumannii isolates (40%) while percentage of other samples were variable. Susceptibility of 15 A.baumannii isolates were tested toward 16 different Antimicrobial agents, the results showed all isolates were multi drug resistant. In addition, Polymerase Chain Reaction Technique (PCR) was performed to detection the resistance genes encoding the Oxacillinases enzymes. The PCR analysis showed that the presence of insertion sequ

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Publication Date
Sun Dec 09 2018
Journal Name
Baghdad Science Journal
A Comparative Efficiency Study of Two Adsorbent Materials to Remove Eosin Y Dye from Aqueous Solutions

This study was done to find a cheap, available and ecofriendly materials that can remove eosin y dye from aqueous solutions by adsorption in this study, two adsorbent materials were used, the shells of fresh water clam (Cabicula fluminea) and walnut shells. To make a comparison between the two adsorbents, five experiments were conducted. First, the effects of the contact time, here the nut shell removed the dye quickly, while the C. flumina need more contact time to remove the dye. Second, the effects of adsorbent weight were examined. The nut shell was very promising and for all used adsorbent weight, the R% ranged from 94.87 to 99.29. However C. fluminea was less effective in removing the dye with R% ranged from 47.59 to 55.39. The thi

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Publication Date
Wed Jan 01 2020
Journal Name
Journal Of Biotechnology Research Center
Molecular screening of the entA gene of Enterococcus faecium isolated from Food and clinical sources

Background: The microbial production of substances that have the potency to suppress the growth of other microorganisms is probably one of the prevalent defense strategy developed in nature, microorganisms produce a variable bunch of microbial defense systems, which include antibiotics, metabolic by-products, lytic agents, bacteriocins and others. Objective: The purpose of the present study was to isolate and identify Enterococcus faecium isolates then detecting its ability of carrying the gene responsible for enterocin production in this species. Materials and methods: Out of 50 samples from different sources (food and clinical sources) were collected for the Enterococcus faecium isolation, and the isolated bacteria Enterococ

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Publication Date
Thu Apr 01 2021
Journal Name
Iraqi J Of Agricultural Science
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Publication Date
Thu Feb 01 2018
Journal Name
Iosr Journal Of Pharmacy And Biological Sciences
Histopathological Study about effect of T.verrucosum on skin of the rabbits and treated by yellow sap and gel of Aloe vera

Background : The dermatophytesare taxonomically related fungi causing different skin infections referred to as tineas in man or ringworm in man and animals . Objective:This study was established to investigate theof effect of yellow sap and gel of Aloe vera on skin of the rabbits which infected with T.verrucosum. Methods : Twelveskin biopsy were taken from rabbits for histopathological study to know the the effects of the T.verrucosumand treated with yellow sap and gel. Results : the results of histopathological study of effect of yellow sap and gel of Aloe vera on skin of the rabbits which infected with T.verrucosum showed the concentration of the gel of aloe vera at 75% was more effected to treat the infective area of skin with T.verrucos

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Publication Date
Tue Aug 31 2021
Journal Name
Iraqi Journal Of Science
Biochemical and Genetic Study in Blood of β– Thalassaemia Children in Mosul City, Iraq

The present study aimed to demonstrate the extent to which the activity of a number of enzymes and genetic variation of β-globin genes were affected in the blood of 65 children with β - thalassemia major of both sexes. The patients, with an age range of 2 – 15 years, were registered in the Thalassemia Center at Ibn Al-Atheer Teaching Hospital for Children in the city of Mosul / Iraq. They were under continuous treatment after being diagnosed by specialist doctors. The study also involved 30 healthy children of both sexes with the same age range who were considered as a control group.

       The results showed significant increases (p≤0.05) in the activities of alanine transaminase (ALT), aspart

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Publication Date
Wed Apr 07 2021
Journal Name
Journal Of Legal Sciences
Substitution as a consequence of liability arising from breach of the promise of preference: A comparative study

     The judicial transformation led by the French Court of Cassation about the consequence of liability arising from breach of the promise of preference led to the adoption of a new principle، whereby the beneficiary of the promise of preference would substitute with the third party in the concluded contract in violation of his right in preference. This shift، in turn، has been reflected on the legislative situation in France، where the French legislator adopted this principle in the New Contract Law of 2016.        

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Publication Date
Sun Mar 01 2015
Journal Name
International Journal Of Computer Science And Mobile Computing
Publication Date
Sun Jun 07 2015
Journal Name
Baghdad Science Journal
A comparative study between conventional methods and Vidas UP Salmonella (SPT) to investigate salmonella species from local and imported meat

The study was preformed for investigating of Salmonella from meat, and compared Vidas UP Salmonella (SPT) with the traditional methods of isolation for Salmonella , were examined 42 meat samples (Beef and Chicken) from the Local and Imported From local markets in the city of Baghdad from period December 2013 -February 2014 the samples were cultured on enrichment and differential media and examined samples Vidas, and confirmed of isolates by cultivation chromgenic agar, biochemical tests ,Api20 E systeme , In addition serological tests , and the serotypes determinate in the Central Public Health Laboratory / National Institute of Salmonella The results showed the contamination in imported meat was more than in the local meat 11.9% and 2

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Publication Date
Wed Jan 30 2013
Journal Name
Al-kindy College Medical Journal
Regeneration of Pain, Touch, Cold, and Warmth, sensations in split thickness human skin grafts in adults (A clinical study)

Back ground: Skin grafting is the most common form
of reconstructive surgery, and regeneration of
sensations in skin grafts is a complex process
influenced by many factors such as , the thickness of
the graft, the depth of the grafted bed, meshing of the
graft, the condition of the bed and the surrounding
area. So many studies performed on this subject, some
of them clinically based on subjective type of sensation
tests, and others histological to detect the presence of
nerve fibers in the grafted skin
Objectives: To detect return of sensations to split
thickness skin grafts by clinical methods.
Methods: From Oct. 1995 to Oct. 2010, a clinical
prospective study performed in Al wasity Hospital for

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