This study includes collection of 70 swabs samples of burns from patients were
admitted in three hospitals (Baghdad, Al- Numaan and burns injuries Hospital). All
swabs samples were cultured on blood and MacConkey agar media to isolate and
identify pathogenic bacteria according to their morphological , biochemical and
growth characters. Growth of bacteria on selective media showed the following
results: Pseudomonas aeroginosa 44.28% , Klebsiella pneumonia 30% ,
Staphylococcu saureus 8.57% , Escherichia coli 4.28% , Proteus vulgaris 4.28 % ,
Enterobacter spp. 5.71% , Acinetobacter baumanni 2.89 %. Different concentrations
were prepared from leaves ethanolic crude extract of Catharanthus roseus , then the
anti-bacterial activity of this extract was evaluated in vivo. Burns were achieved
experimentally in Albino mice and contaminated with pathogenic bacteria and
evaluate the ability of extract in healing of these infected burns. Results showed that
the extract accelerate burns healing within 7days.
Abstract
The experiment has been carried out in the Syrian National Commission of Biotechnology, during the growing season 2018/2019, to study the effect of abiotic stresses (salinity and osmotic stresses) on the activity of some antioxidant enzymes and biochemical traits in Catharanthus roseus. The experiment has been laid according to (CRD) with three replications. The seeds have been sterilized by NaOCl solution (0.5% v/v), then planted on MS medium. Plantlets have been moved to MS medium enriched with NAA (1 mg.L-1) and BA (2 mg.L-1). The callus has been initiated from leaves using MS medium containing NAA (1 mg L-1) and KIN (2 mg.L-1). After 60 days, callus
... Show MoreAn increasing trend to use probiotic and study their effects on the pathogens has been conductor where they are defined as live micro-organisms that give a health benefit to the host when ingested in sufficient quantities, including the yeast Saccharomyces boulardii In addition research show that a magnetic field (MF) has a biological effect. This study aims to investigate the effects of magnetic field on the inhibitory action of Saccharomyces boulardii against bacteria isolated from urinary tract infection, Study the sensitivity of bacterial isolates to antibiotics after diagnosis by microscopic, Cultural and biochemical examinations as well as Api20 E examinations were used gram negative bacteria , Most isolates were resistant to an
... Show MoreThe present study was conducted to reveal the effect of crude oil on some fungal species isolation from soil in order to evaluate the role of these fungi in environmental balance of soil . The results showed a variation in numbers and percentage of the fungal isolates Aspergillus fumigatus dominated over all isolates with a frequency of (32.47) . In respect of the effect of different concentrations of the crude oil, low concentrations (0.05, 0.1) % showed no effect on radial growth ( mean colony diameter) of the isolated fungi grown &nbs
... Show MoreSoil bacteria play an interesting role in the reduction of Ag+ ions and the formation of silver nanoparticles (AgNPs), which may be a good source for nanoparticles and play a major role in nanotechnology applications. The concept of this project was to study the effects of these environmentally produced nanoparticles on the growth of some pathogenic bacteria. The environmental bacteria were isolated from soil, purified on broth cultures, and centrifuged, while the supernatant was extracted to detect its ability to convert silver nitrate to nanoparticles. The AgNPs was detected by Atomic Force Microscopy (AFM), while Granularity Cumulating Distribution (GCD) was employed to estimate the AgNPs sizes. The results showed the
... Show MoreBackground: The prevalence of multidrug-resistant bacteria and their contribution to increased morbidity and mortality due to the difficult-to-treat diseases caused by these bacteria, has demonstrated a need to develop and use alternative antimicrobial agents to control multidrug-resistant bacteria. There has been a growing interest in medicinal plants and herbs and their extracts for the discovery of new natural therapeutic alternatives. Therefore, this current study aimed to know the antibacterial activity of aqueous and alcoholic extracts of the Sumac (Rhus coriaria L) fruits against multidrug-resistant clinical bacterial isolates and the effect of these extracts on biofilm production as an important virulenc
... Show MorePlants and their extracts preparations have been used as medicines against infectious diseases. In present work, Cassia senna (leaves), Piper nigrum (fruits) were extracted with different organic solvents to investigate their antifungal activities in vitro. However, the effective of plant extracts against some pathologic fungi (Tricophyton rubrum, T. tonsurans, T. violaceum, Microsporum audouinii, M. canis and M. gypseum) were evaluated at concentrations ranged between (0.005–5%) using agar diffusion methods and compared with standard antifungal drug (Clotrimazole). Results showed that methanol extract of C. senna and ethanol extract of P. nigrum displayed excellent inhibition on dermatophytes compared with standard antifungal drug, th
... Show MoreThe objective was to study the effect of prepared ginkgo biloba extracts against Candida albicans isolated from healthy persons. Conducting susceptibility test, biofilm formation test, phytochemical screening test, and antioxidant activity test. One hundred oral swabs sample were obtained from healthy persons with oral lesion attending dentistry teaching hospital in dentistry college, their age ranged from 1-30 years of both sexex. The studied samples collected through 8 months (April - December / 2018). This study included two different types of ginkgo bilola extracts were prepared as aqueous and ethanolic extracts. Many tests were used, which included isolation and identification of C.albicans, conduct susceptibility test, biofilm form
... Show MoreIn this study Isolated Pathogenic bacteria which causes Tonsillitis in Children with ages between 3-17 years. They are admitted to Central Children Hospital (Al-Karch) and Ebn-Albalady Hospital (Al-Rusafa). 200 cases were collected which include 120 Male and 80 Female. The result of the recent study shows that the isolation percentage was 40% from Male and 35% from Female. In this study Fifty six isolated were Identified, 20 were ?-hemolytic Streptococcus which was Streptococcus pyogenes, formed (36%) from all isolated.6 Pathogenic bacteria were ?- hemolytic Streptococcus which was Streptococcus pneumoniae formed (11%). The number of Moraxella catarrhalis bacteria was 12 formed (21%), the number of Haemophilus influenzae was 1
... Show MoreThis study was conducted in Wasit governorate for the period between February 2012 to February 2013 to determine the impact of Castor seed oil at different concentrations 100, 75, 50 and 25% in inhibition of the growth of E. coli isolated from 52 male and female patients (2-70 yrs) attending Al Zahra Hospital and Al Karama Hospital in Kut city. Oil was extracted from seeds of Castor had the ability to inhibit E. coli isolated from patients presented with recurrent urinary tract infections . Zone of inhibition accomplishing was 9.06 mm in diameter.HPLC analysis revealed that the content of α-linolenic in Castor seed oil (18.90 μg\ml) was higher than other fatty acids followed by oleic. Perhaps this is why it able to inhibit E. coli; whi
... Show MoreA study were conducted to examinate the effect of organic and aqueous (Hot, Cold) Extracts from leaves of Duranta repens on the growth and activities of the following types of Bacteria:- Staphylococcus aureus,Streptococcus pyogens ,Escherichia coli,Klebsilla pneumonia, in addition to the yeast Candida albicans and the fungi Aspergullis niger ,Aspergulls flavus.The result showed that gram Positive Bacteria is more sensitive to the extracts than gram negative bacteria with Minimum inhibitory concentration (MIC) value (50,25,50,100)% and Minimum Bactericidal Concentration (MBC) value (100,50,200,100)% for all types Bacteria respectively . The most active extract against A.niger ,A,flavus was cold and hot aqueous extract from the leaves with d
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