A total of 47 samples were collected from different clinical specimens (urine, wounds, burns, sputum, blood, stools) during the period from November 2013 to
January 2014, only 18 isolates (38.29%) were identified as Klebsiella pneumoniae ,11isolates (23.4%) as E. coli , 9 isolates (19.1%) as S. aureus, 3isolates (6.3%) as Psedomonase spp., 2 isolates (4.2 %) as K. terrigena and 4 isolates (8.5%) as K. oxytoca. The results were shown the elevation of K. pneumoniae percentage among the bacterial isolates depending on cultural, microscopically, biochemically characteristics and confirmed by using the API 20E and VITEK 2 system . Also K. pneumoniae isolates were more frequently in sputum samples then burns, stools , urine, wounds ,and blood in different percentages (33.3%, 16.6%, 16.6%, 11.1%,
11.1%, 11.1%) respectively. The in vitro sensitivity tests of K. pneumoniae isolates against (19) antimicrobial agents were determined through disc-diffusion method. It was found that all isolates were 100% resistant to (Ampicillin /Cloxacillin, Amoxicillin, Carbencillin, Oxacillin, Penicillin G and Ceftazipime, whereas they indicated variable resistance to the other antibiotics used. While Amikacin , Gentamicin and Imipenem revealed that were the most effective drugs used in the present study. Moreover K. pneumoniae isolated from sputum, urine and blood samples are more resistant to antimicrobial agents at percentages (78.9%), the most resistance isolates, 6 isolates were selected .The molecular detecting of some capsular polysaccharide genes (cps) were investigated by using PCR specific primers. Genotyping of 6 selected K. pneumoniae isolates were indicated by using K1,K2 specific PCR primers. The results were shown ,that there is no isolate belong to K1 serotype, while 4 isolates (66.6%) are belonged to K2 serotype, and 2
isolates (33.3%) showed negative result for both K1/K2.
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FH Ghanim, Journal of Global Pharma Technology, 2018
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Biological activity substances was investigated in watery extract of lentil which found to contain phenols, tannin, saponins and resins while, flavons, terpens and steroids were not exist in the extract details explained that 5%, 10% of lentil extract largly inhibited the growth of Psedumonas aeruginosa then Escherichia coli and Bacillus subtilis. The growth of both Staphylococcus aureus and Salmonella typhimurium were slightly affected by all extract concentration. Extracellular protease were screened in all bacterial species under study. Complete inhibition was achieved for extracellular protease while different percentage of protease inhibition were seen for intracellular proteases.
The aim of this study was to evaluate the possibility of using Staphylococcus epidermidis cells as a probiotic to treat some skin infections. For this purpose, S. epidermidis Y73, which is an active bacteriocin producer and non-biofilm forming isolate, was selected among 134 skin isolates through primary and secondary screening. Tryptic soya broth was selected as the best medium to support bacteriocin production, while the optimal pH and temperature for S. epidermidis Y73 growth were 7 and 37°C, respectively, which were invested in the formula preparation. Furthermore, the possibility of using this isolate as a probiotic was investigated by preparing 4 potential cellulosic pads with 4 different formulae whic
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This research includes the synthesis of some new different heterocyclic derivatives of 5-Bromoisatin. New sulfonylamide, diazine, oxazole, thiazole and 1,2,3-triazole derivatives of 5-Bromoisatin have been synthesized. The synthesis process started by the reaction of 5-Bromoisatin with different reagents to obtain schiff bases of 5-Bromoisatin intermediate compounds(1, 8, 19) by using glacial acetic acid as a catalyst in three routes. The first route, 5-Bromoisatin reacted with p-aminosulfonylchloride to product compound(1), then converted to sulfonyl amide derivatives(2-7) by the reaction of compound(1) with different substituted primary aromatic amine in absolute ethanol. The second route includes the reaction of 5-Bromoisatin rea
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