A newly developed analytical method characterized by its speed and sensitivity for the determination of metoclopramide hydrochloride (MCP-HCl) in pure and pharmaceutical preparation via turbidimetric measurement (0-180o) by Ayah 6SX1-T-2D Solar cell-CFI Analyser. The method was based on the reaction of phosphomolybdic acid with metoclopramide hydrochloride in acidic medium to form yellowish white precipitate for the ion-pair complex. Turbidity was measured via the reflection of incident light that collides on the surface precipitated particles at 0-180o. Chemical and physical parameters were studied and optimized. The calibration graph was linear in the range of 0.0005-3 or 0.0005- 4 mMol.L-1, with correlation coefficient r = 0.9947&0.9845 respectively. The limit of detection 3.543 ng/sample from the step wise dilution for the minimum concentration in the linear dynamic ranged of the calibration graph with RSD% lower than 0.3% for 1, 3 mMol.L-1 (n=8) concentration of metoclopramide hydrochloride . The method was successfully applied to the determination of metoclopramide hydrochloride in three pharmaceutical drugs. A comparison was made between the newly developed method analysis with the classical method (HANNA instrument for turbidity measurement) using the standard addition method via the use of t-test. It was noticed that there was no significant difference between two methods at 95 % confidence level.
A simple, rapid and sensitive spectrophotometirc method for the determination of trace amounts of promethazine hydrochloride in the aqueous solution is described. The method is based on the complexation of promethazine hydrochloride with In (III) in the presence of sodium hydroxide to form an soluble product with maximum absorption at 304nm. Beer’s law is obeyed over the concentration range of (2- 20μg/ml) with molar absorptivity of (1.92× 103 L.mol-1 .cm -1 ). The optimum conditions for all development are described and the proposed method has been successfully applied for the determination of promethazine hydrochloride in bulk drug.
A new approach and the developed FIA technique with many advantages (economic, fast, simple, accurate, and high throughput) are used to determine the decongestant drugs (Phenylephrine.HCl, Oxymetazoline.HCl) in biological samples, pharmaceutical formulations, and pure samples via continuous flow injection technique by oxidative coupling reaction, where the method depends on the interaction of the decongestant drug with organic reagents to produce colored compounds, where Phenylephrine reacts with 4-AAP at λmax503 nm to produce a red compound, and the Beer’s law range of 10-600 μg.mL-1 . As for Oxymetazoline, it reacts with DNPH at λmax 631nm to produce a green compound with a linear dynamic range of
... Show MoreSimple and rapid spectrophotometric determination of furosemide (FUR) has been investigated .The method is based on acid hydrolysis of FUR to free primary aromatic amine and diazotization followed by coupling with 3, 5 di methyl phenol (3, 5-DMPH) at basic medium. The absorbance was measured at 434 nm, the method was optimized for best condition, and beers’ law is obeyed over the range of 0.4-50 µg.mL-1 with molar absorptivity and sandal’s sensitivity 1.3899 x104 L moL-1 .cm-1 and 0.0238x104 µg.cm-2 respectively. Analysis of solution containing nineteen different concentrations of FUR gave a correlation coefficient of (0.9999) a
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A newly developed analytical method characterized by its speed and sensitivity for the determination of mefenamic acid (MFA) in pure and pharmaceutical preparation is established via turbidimetric measurement (0-180o) by Ayah 6SX1-ST-2D Solar cell CFI Analyser . The method was based on the reaction of
phosphomolybdic acid with mefenamic acid in aqueous medium to form blue color precipitate as an ion-pair complex . Turbidity was measured via the reflection of incident light that collides on the surface precipitated particles at 0-180o . The chemical and physical parameters were studied and optimized. The calibration graph was linear in the range of 0.3-7 or 0.3-10 mMol.L-1, with correlation coefficient r = 0.9907 or 0.9556 respectively
Inhibition of T cell derived cytokine production could help suppress osteoclast differentiation in inflammatory skeletal disorders. Bisphosphonates are typically prescribed to prevent inflammatory bone loss but are not tolerated by all patients and are associated with an increased risk of osteonecrosis of the jaw. In light of this other anti-resorptives such as phytoestrogens are being considered. However the effect of phytoestrogens on T cell-induced osteoclast formation is unclear. The effect of genistein and coumestrol on activated T cell-induced osteoclastogenesis and cytokine production was therefore examined. Concentrations of genistein and coumestrol (10−7 M) previously shown to directly inhibit osteoclast formation also suppressed
... Show MoreThe determination of captopril (CAP) using a new continuous flow injection analysis (CFIA) method was given in this work CAP in its pure state and some of its pharmaceutical preparations. The technique can be described as simple, fast, sensitive, easy to operate, and low-cost. The CAP reacted with ammonium ceric(IV) sulfate (ACS)2(NH4 )2SO4Ce(SO4)2. 3 H2O in an acidic medium and the reaction led to the formation of a white, slightly yellowish precipitate. The formed precipitate was studied using Ayah 6S×1-ST-2D Solar cell-CFI Analyzer, a through the reflection of accident light on the surfaces of the precipitate particles at (0-1800), expressed as the response
... Show MoreLiquid membrane electrodes for the determination iron(III) were constructed based on chloramphenicol sodium succinate and iron(III) CPSS-Fe(III) as ion pair complex, with four plasticizers Di-butyl phosphate (DBP); Di-butyl phthalate (DBPH); Di-octyl phthalate (DOP); Tri-butyl phosphate (TBP); in PVC matrix . These electrodes give Nernstian and sub-Nernstian slopes (19.79, 24.60, 16.01 and 13.82mV/decade) and linear ranges from (1x10-5-1x10-2 M, 1x10-5-1x10-2 M, 1x10-6-1x10-2 M and 1x10-5-1x10-2 M) respectively. The best electrode was based on DBP plasticizer which gave a slope 19.79 mV/decade, correlation coefficient 0.9999, detection limit of 9×10-6 M, lifetime 37 day displayed good stability and reproducibility and used to determine
... Show MoreA rapid, simple and sensitive spectrophotometric method for the determination of trace amounts of chromium (VI) was studied. The method is based on the reaction of chromium (VI) with promethazine forming a red colored species by chromium in hydrochloric acid medium and exhibits a maximum absorbance at 518 nm. A plot of absorbance with chromium (VI) gives a straight line indicating that Beer’s law has been obeyed over the range concentration of 0.05-4.0 µg/ml with a molar absorptivity of chromium(VI) 2.04  104 l.mol-1.cm-1 , Sandell’s sensitivity index of 0.0025 µg.cm-2 while the limit of detection (LOD) was found to be 0.0924 µg.ml
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