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PDF Sensing and differentiation between normal flora and pathogenic of E.coli Bacteria using 410 nm diode laser

Abstract: Background: Optical biosensors offer excellent properties and methods for detecting bacteria when compared to traditional analytical techniques.  It allows direct detection of many biological and chemical materials.  Bacteria are found in the human body naturally non-pathogenic and pathologically, as they are found in other living organisms.  One of these bacteria is Escherichia coli (E. coli) which are found in the human body in its natural and pathogenic form. E.coli bacteria cause many diseases, including Stomach, intestines, urinary system infections, and others. The aim of this study: is sensing and differentiation between normal flora and pathogenic E.coli. Material and method: The optical biosensor constructed of a multi-mode – no core- multi mode optical fibre that differentiates between pathogenic and non-pathogenic bacteria of E.coli by measuring the changing for light intensity using source of light 410nm laser diode.  Multi-mode - no core - multi-mode optical fibre (MM-NOC-MM) connected to the OSA analyser (HR2000) by means of an adapter and finally connected to a computer to show the results. Results: The intensity of the transmitted light recorded in the case of pathogenic bacteria is less than the intensity of the transmitted light recorded in the case of non-pathogenic bacteria. Conclusion: these results were obtained because of the ideal and better choice of the wavelength of the laser used with its absorption E.coli bacteria.

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Publication Date
Tue Nov 19 2024
Journal Name
Iraqi Journal Of Science
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Publication Date
Sat Dec 17 2022
Journal Name
Iraqi Journal Of Laser
PDF Cavity Disinfection Using Er,Cr:YSGG Laser Induced Photoacoustic Streaming Technique: Rand kareem Jassim Hussein Ali Jawad*

Abstract:

Aim: The goal of this research was to study the influence of Er,Cr:YSGG laser at short pulse duration (60 µsec) on the number of streptococcus mutans bacteria in vitro.

Material and Methods:  twenty-eight extracted third molars free of caries, cracks, and other irregularities were used. For the testing of the materials, both the agar well technique and a tooth cavity model were employed. The agar wells of plates that had been inoculated with Streptococcus mutans previously were stuffed with the test materials, in order to conduct the tests. The zones of inhibition were assessed using millimeter measurements, after an incubation period of 48 hours .In order to a

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Publication Date
Thu Feb 07 2019
Journal Name
Iraqi Journal Of Laser
In Growing Toenail Treatment using Carbon Dioxide Laser

An ingrowing toenail is a common problem affecting mainly adolescents and young adults, with a male predominance of 3:1. The disorder generally occurs in big toes. It is painful and often chronic and it affects work and social activities. Most patients initially complain of pain and later discharge, infection and difficulty in walking occur. The Objectives: The purpose of the study was to evaluate the efficacy and safety of (10600nm) CO2 laser in the treatment of ingrowing toe nail. Patients, Materials & Methods: This study was done in laser medicine research clinics from July 2013 to the end of December 2013; 10 patients including 7(70%) males and 3 (30%) females with age ranging from 18 years to 70 years with mean age of 44 years o

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Publication Date
Fri Feb 08 2019
Journal Name
Iraqi Journal Of Laser
Sealing Depth of Q-Switched Nd:YAG 1064nm Laser in the Treatment of Patients with Hypersensitive Teeth: An In Vitro Study

The purpose of this work was to study the effects of the Nd:YAG laser on exposed dentinal
tubules of human extracted teeth using a scanning electron microscope (SEM). Eighty 2.5mm-thick
slices were cut at the cementoenamel junction from 20 extracted human teeth with an electric saw. A
diamond bur was used to remove the cementum layer to expose the dentinal tubules. Each slice was
sectioned into four equal quadrants and the specimens were randomly divided into four groups (A to D ).
Groups B to D were lased for 2 mins using an Nd:YAG laser at 6 pulses per second at energy outputs of
80 , 100 and 120 mJ. Group A served as control. Under SEM observation, nonlased specimens showed
numerous exposed dentinal tubules. SEM o

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Publication Date
Thu Jan 07 2021
Journal Name
Jordan Journal Of Biological Sciences
Evaluation of Quorum-Sensing, Antibiotics Resistance, and Biofilm Formation in Pathogenic Bacteria from the Hospital Environments

Abstract Background: Multidrug-resistant bacteria (MDR) often contaminate hospital environment and cause serious illnesses. Quorum Sensing (QS) regulates a variety of downstream cellular processes, including antibiotics resistance mechanisms and biofilm formation, and causes harm to the host. This study investigates antibacterial susceptibility and biofilm formation of pathogenic bacteria in hospital environment. Methods: Hundred bacterial isolates were collected from various environments in the Medical City hospital. The antimicrobial susceptibility technique was evaluated through disk diffusion method. Next, biofilms formation was detected by the microliter plate assay. Finally, PCR was used to analyze the frequency of QS system gene

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Publication Date
Tue Dec 13 2005
Journal Name
Iraqi Journal Of Laser
Clinical Observation and Performance of High Power Diode Laser in the Treatment of Oral Lesions

In this work, oral lesions belong to 17 patients, 7 males and 10 females. Their ages range between 15 and 45 years. Follow up was conducted after one day, 7 days, 14 days, one month, and finally 3 months postoperatively. The study lasted for 1.5 year. Surgical diode laser with wavelength of 810 ± 20 nm, with two power levels of 10 and 15 W were used in contact and in non-contact mode via optical fiber. The postoperative outcome revealed; greater haemostatic capability, dry, sealed wound and noticeable lack in pain sensation

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Publication Date
Tue Jun 01 2010
Journal Name
Al-khwarizmi Engineering Journal
355nm Wavelength Generation of Nd:YAG Laser Using Olive Oil

 This project introduces a prospective material for photonic laser applications. The material is olive oil which is classified as organic compound, having a good nonlinear optical properties candidate to be used in photonic applications. A high purity sample of olive oil has been used. The theoretical calculation to generate third harmonic wave using olive oil has been determine using MATLAB program. THG (λ=355nm) intensity has been determined at two cases of sample thicknesses 1mm and 10mm. The minimum threshold incident intensity to obtain THG intensity are equal Iω=7530 mW/cm2 at L=1mm and Iω= 6220 mW/cm2 at L=10mm. The possibility of generation of third harmonic in olive oil inside

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Publication Date
Fri Jan 01 2021
Journal Name
Materials Today:proceeding
Scopus (2)
Scopus
Publication Date
Tue Jul 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The association between abnormal vaginal microbial flora and preterm labor. Does a simple Gram's stain of vaginal swab gives more useful data than culture ?

Background: Preterm labor is the major cause of perinatal mortality and morbidity and it is a challenging obstetrical complication as treatment is marginally effective if ever and no improvement in incidence over the last 5 decades , although may causes were identified the majority are idiopathic and in this category infection seems to be involved as a causative agent

Objectives: to evaluate the association of abnormal vaginal flora diagnosed by

Fac Med Baghdad

2014; Vol.56, No.2

Received: Jan., 2014

Accepted March. 2014

 

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Crossref
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Publication Date
Mon Oct 17 2022
Journal Name
Journal Of The Faculty Of Medicine Baghdad
the Antibacterial and anti-biofilm effects of Sumac (Rhus coriaria L) fruits extracts against some multidrug-resistant pathogenic bacteria: Antibacterial and antibiofilm effect of sumac (Rhus coriaria L) fruits extracts against some multidrug_resistant pathogenic bacteria

Background: The prevalence of multidrug-resistant bacteria and their contribution to increased morbidity and mortality due to the difficult-to-treat diseases caused by these bacteria, has demonstrated a need to develop and use alternative antimicrobial agents to control multidrug-resistant bacteria. There has been a growing interest in medicinal plants and herbs and their extracts for the discovery of new natural therapeutic alternatives. Therefore, this current study aimed to know the antibacterial activity of aqueous and alcoholic extracts of the Sumac (Rhus coriaria L) fruits against multidrug-resistant clinical bacterial isolates and the effect of these extracts on biofilm production as an important virulenc

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Crossref
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