Abstract: Background: Staphylococcus aureus is Gram-positive bacteria that lives as a normal flora in living organisms but can be pathogenic to humans. Although a relatively unspectacular, nonmotile coccoid bacterium, S. aureus is a dangerous human pathogen in both community-acquired and nosocomial infections. Due to the increasing emergence of new strains of this antibiotic-resistant bacteria, it has become essential to approach different methods to control this pathogen. One of these methods is the antimicrobial photodynamic inactivation process using a low-level laser, in this paper, the Photodynamic effects of Rose Bengal and LLLL on the virulence factors of S.aureus were evaluated. The aim of the study The present study aims to evaluate the Photodynamic effects on S. aureus using laser irradiation and Rose Bengal as an external photosensitizer. Methods: sixty samples from sputum were taken. Then ten isolated from these samples were chosen to be under the study, where RB was used at a concentatin of 100 μg/ml that is activated by diode laser (532 nm) with power density of 1 W/m2and exposure time (1, 2& 3) minute. Results: show that there is no effect on the inhibition of virulence factors except at the last minute, that is, the virulence factors decrease at the third minute only in the absence of a photosensitizer agent, while there is a direct effect of activated Rose Bengal on S. aureus isolated from the sputum of Iraqi patients with pneumonia, where all times of exposure of (RB + 532 nm) were effect on the virulence factors by inhibiting it. Conclusions: show that the diode laser of 532 nm has no effect on the virulence factor of S. aureus isolated from sputum except at the third minute, while RB activated by diode laser (532 nm) have an effective action on all virulence factors of S. aureus isolated from sputum at all times of exposure, accordingly, it was concluded that when using a laser diode alone, the bacterial viability decreases at the third minute only, While when using Rose Bengal activated by a diode laser, the viability of bacteria is reduced at all times of exposure.
Background: One of the most dominant bacterial infections is urinary tract infection (UTI), both in the community and in the hospital settings Since drug resistance become one of the predominant problems of health worldwide, it is necessary to use new methods to overcome drug-resistant bacteria. In this regard, medicinal plants (Medicago sativa ) are considered one of the richest sources to produce antibiotics.
Aim of the study: evaluate the antibacterial effect of the fraction of ethyl acetate of Medicago Sativa extract against Escherichia Coli isolated from urine samples of patients with urinary tract infections.
Material and methods:
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The biological effects of pulsed N2-laser on the old world screw-worm fly, Chrysomya bezziana Villeneuve in the pupal stage were investigated. Different laser parameters were involved in this work. The old pupae of 1, 2, 3, 4 and 5 days were exposed to laser radiation during 10, 30 and 60 second with repetition rate 10, 20 and 30 pulse/second. The percent of normal adults emergence (female and male) was investigated. The results showed that the adults emergence was highly decreased as the repetition rate and exposure time increased when the pupae irradiated for 1, 2 and 3 days old as compared with 4 and 5 days. The results also indicated that the pupal period was significantly increased of irradiated pupae for 1, 2, 3 and 4 days old, whi
... Show MoreSKF Sami I. Jafar, Mohammad J. Kadhim, Engineering and Technology Journal, 2018 - Cited by 4
A theoretical study has been proposed to investigate the effects of different laser radiations (Nd - glass, DF and C02) as a heating source on different glass samples (Optical glass, Bk - 7 and Soda - lime glass) and different waves lengths (10.6, 3.8, 1.6) ???. The heat changes as which are resulted due irradiation with laser sources have been determined by using the one dimension mathematical relation as a function of time (t) and depth (z). The results of the study show ed that the irradiation with C02 laser had a greater effect than DF laser, while the effects of Nd - glass laser were minimal with a power density of (1.8*10?? w/m2) within atime(l^sec).(Forboth Kinds) The change in the temperatures were not exceeded than (70"K) in all sa
... Show MoreNowadays laser in medicine is a rapidly growing field in both researches and applications and many studies have been done in bacteriology against different types of lasers. The effect of the laser depends on many factors, one of the laser factors was the wavelength. The red wavelength band has been considered as a stimulated wavelength for prokaryotic and eukaryotic cells. In this study, three clinical species of Gram-negative bacteria (E. coli, Proteus mirabilis, and Pseudomonas aeruginosa) were exposed to 650 nm band of red region wavelength by a diode laser. The effect of that wavelength appeared on the growth curves for each species along 3 days after laser treatment. The energy density (E.D.) 2.8 J/cm2 gave a positive effect on grow
... Show MoreIn this work, nanostructured TiO2 thin films were grown by pulsed laser deposition (PLD) technique on glass substrates. TiO2 thin films then were annealed at 400-600 °C in air for a period of 2 hours. Effect of annealing on the structural and morphological were studied. Many growth parameters have been considered to specify the optimum conditions, namely substrate temperature (300 °C), oxygen pressure (10-2 Torr), laser fluence energy density (0.4 J/cm2), using double frequency Q-switching Nd:YAG laser beam (wavelength 532nm), repetition rate (1-6 Hz) and the pulse duration of 10 ns. The results of the X-ray test show that all nanostructures tetragonal are polycrystalline. These results show that grain size increase fr
... Show MoreCarbon nanoparticles (CNPs) formed by one-step laser ablation in deionized water were carefully studied. Scanning electron microscopy, atomic force microscopy, Raman spectroscopy, and UV–V spectroscopy were used to obtain morphological, chemical, and optical properties of CNPs. SEM outcomes established that the synthesized nanoparticles are semi-spherical with a wide particle size distribution. Raman investigation showed two typical and expected peaks ~ (1300 - 2700) cm−1, which are confirming to transverse and longitudinal modes of the carbon structure. The absorption spectra proved that the intensity of spectra increases as particle size and concentration increase.
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Background: Phosphodiesterase-5 (PDE-5) inhibitorsrestore nitric oxide (NO) signaling and may reducecirculating inflammatory markers, and improve metabolicparameters through a number of mechanisms. Dailyadministration of the PDE-5 inhibitor, tadalafil (TAD) mayattenuate inflammation; improve fasting plasma glucose andtriglyceride levels and body weight. This study aims toevaluate the efficacy of low dose PDE-5 inhibitor, tadalafil(TAD) in controlling dysglycemia and body weight in obesediabetic men.Methods: Forty obese men with type 2 diabetes aged 30-50years incorporated in this study, all with A1c of 7-8.5%,attending obesity unit in AL-Kindy college of medicine.Weight, height, BMI, FPG, A1c, cholesterol, TG, HDL andLDL measured for all
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