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PDF Cavity Disinfection Using Er,Cr:YSGG Laser Induced Photoacoustic Streaming Technique: Rand kareem Jassim Hussein Ali Jawad*
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Abstract:

Aim: The goal of this research was to study the influence of Er,Cr:YSGG laser at short pulse duration (60 µsec) on the number of streptococcus mutans bacteria in vitro.

Material and Methods:  twenty-eight extracted third molars free of caries, cracks, and other irregularities were used. For the testing of the materials, both the agar well technique and a tooth cavity model were employed. The agar wells of plates that had been inoculated with Streptococcus mutans previously were stuffed with the test materials, in order to conduct the tests. The zones of inhibition were assessed using millimeter measurements, after an incubation period of 48 hours .In order to accomplish the tooth cavity model test, cylindrical cavities were invented in the occlusal surface of the teeth, which was kept even. The teeth were stored for 72 hours at 37°C in a broth culture of Streptococcus mutans. Following this, the teeth were divided arbitrarily into four groups of seven teeth (each including 14 cavity preparations). The experimental cavities in the first group (A) were not treated and considered as a control. In group B, a cavity disinfectant based on chlorhexidine was applied to the experimental cavities for 60 seconds. In group C, an erbium, chromium doped yttrium scandium gallium garnet laser was used at a short pulse duration (60 µsec) (0.25 watts, 15 Hz, 1% air, 1% water). In the last group, a chlorhexidine cavity disinfectant was applied for 60 seconds, followed by a laser treatment for 30 seconds with the same parameters as those described previously. The teeth were stored in saline for a period of three days. Standard amounts of dentin chips were retrieved from the cavity walls. ANOVA test was used to analyze repeated measure mean between tested concentration and control. Data expressed as mean ±SE.  LSD tests was used to calculate the significant differences between tested mean.

Result: After the statistical test, the highly significant difference in the diameter of inhibition zone was observed in group D (26 mm) where both chlorhexidine and laser were used followed by group B (18.71 mm) where the chlorhexidine gluconate based cavity disinfectant used alone, the least significant difference observed in group C (10.26mm) where the laser used alone.

Conclusion: According to this in vitro study, a photon-induced photoacoustic streaming technique using an Er,Cr:YSGG pulse laser at short pulse duration effectively agitates a chlorhexidine-based cavity disinfectant, which leads to the inhibition of Sterptococcus Mutans.

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Publication Date
Tue Nov 01 2016
Journal Name
Current Science
Histopathological changes in golden hamsters induced by Leishmania tropica
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Publication Date
Thu Jan 07 2021
Journal Name
Indian Journal Of Forensic Medicine & Toxicology
Guggulsterone Suppresses Ovalbumin- Induced Inflammation in Rat Asthmatic Model
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The present article includes an experimental study of the behavior of dry and saturated dense sandy soil under the action of a single impulsive load. Dry and saturated dense sand models were tested under impact loads. Different falling masses from different heights were conducted using the falling weight deflectometer (FWD) to provide the single pulse energy. The responses of dense soils were evaluated at surface of soil under impact load. These responses include; displacements, velocities, and accelerations that are developed due to the impact acting at top and the displacement at different depths within the soil using the falling weight deflectometer (FWD) and accelerometers (ARH-500A waterproof, and low capacity acceleration tran

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Publication Date
Wed Mar 10 2021
Journal Name
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THIAMINE – INDUCED FORMATION OF THE MONOPYRROLE MOIETY OF PRODIGIOSIN
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Thiamine stimulates the production of a red pigment , which is chromatographically and spectrophotometrically identical to prodigiosin , by growing cultures of serratia marcescens mutant 9-3-3 . this mutant is blocked in the formation of 2- methyl -3- amyl pyrorol( MAP),the monopyrrole moiety of prodigiosin , but accumulates 4-methoxy-2, 2-bipyrrole -5- carboxaldehyde (MBC) and can couple this compound with( MAP) to form prodigiosin . Addition of thiamine caused production of( MAP) , and as little as 0.02 mg of thiamine / ml in peptone- glycerol medium stimulated production of measurable amounts of prodigiosin. Phosphate saltes and another type of peptone decreased the thiamine- induced formation of prodigiosin ,yeast extract and glyc

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An In Vitro Histological Study of Human Skin Wound Soldering Using 980 nm Diode Laser in Continuous versus Pulsed Modes.
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Laser skin wound soldering offers many distinct advantages over conventional closure and laser welding techniques. Objective : to compare the histological effects of human skin wound soldering using 50 % human albumin solder and compound charcoal photosensitiser with 980 nm diode laser acting in various modes of action and parameters. Study Design/Materials and Methods: In this in vitro experimental study , Multiple 3-4 cm long full thickness incisions in a specimen of human skin were soldered using a 4 mm spot diameter beam of 980 nm diode laser(at different laser parameters and modes of action) with 50 % human albumin solder mixed with the compound charcoal at 5 % W/V concentration .After obtaining a successful wound soldering , the wo

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Publication Date
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Thu Oct 29 2020
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