Introduction and Aim: Beta-thalassemia is a serious inherited genetic disorder and an increasing health burden globally. Beta -thalassemia is caused by genetic globin abnormalities within the hemoglobin beta (HBB) gene. This study aimed to characterize the HBB gene mutations in beta -thalassemia among southern Iraqi patients. Materials and Methods: The study included 30 beta -thalassemia patients referred to the Thi-Qar Center for Genetic Diseases, Iraq and 15 control samples from a random group of apparently healthy individuals. Genomic DNA was isolated from blood sample collected from each individual. The DNA was amplified for specific regions of the HBB gene and the amplified products sequenced. The sequences generated were analysed for mutations using sequence analysis tools. Results: Molecular analysis revealed several mutations in the HBB gene including translocation, deletion and substitution mutations in the population tested positive for the beta -thalassemia trait. Conclusion: Thalassemia major is a serious concern in southern Iraq and therefore this study emphasizes a need for complete mutation profiling of the beta -globin gene as a strategy for screening of carriers within the population. Such examinations could be useful in pre-marital genetic counseling and for undertaking prevention and treatment measures.
Rice is a major staple food for more than two thirds of the world population. Pathogenesis-related proteins-10 (PR10) have a range of 154 to 163 amino acid with molecular weight ~ 17 kDa. They are acidic and generally intracellular and cytosolic proteins accumulate in plants in response to biotic and abiotic stresses. In the present study, a PR10 gene and its corresponding protein were characterized in O. sativa, O. barthii, O. glaberrima, O. glumipatula, O. meridionalis, O. nivara, O. rufipogon and O. punctata. The results revealed a narrow range of variation at both DNA and protein levels in all examined species except O. glumipatula. The latter showed a relatively
... Show MoreAn aggregate of 30 samples (water, sediments, and plants) was collected from Al-Chibayish Marsh, located in Dhi-Qar Governorate southern of Iraq to investigate the bioaccumulation and biomagnification in marsh plants (flora) and to assess the marsh plants pollution condition. The study was conducted by testing the macro elements, microelements, heavy metals, and organic compositions in water, sediments, and plants. Plant analyses revealed that the Salvinia natans plant species had the highest concentrations of macroelements Mg, Ca, Na, P, and N compared with other marsh plants and sediments. As a result, the cation binds itself to be more than one charged cationic site and this behaviour was observed in Salvinia natans sp. which has the
... Show MoreAnalyze the relationship between genetic variations in the MTHFR gene at SNPs (rs1801131 and rs1801133) and the therapy outcomes for Iraqi patients with rheumatoid arthritis (RA). The study was conducted on a cohort of 95 RA Iraqi patients. Based on their treatment response, the cohort was divided into two groups: the responder (47 patients) and the nonresponder (48 patients), identified after at least three months of methotrexate (MTX) treatment. A polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) technique was employed to analyze the MTHFR variations, specifically at rs1801133 and rs1801131. Overall, rs1801131 followed both codominant and dominate models, in which in
Background: This study evaluate the immunohistochemical expression profile of transforming growth factor beta-1 in inflamed gingival tissue of patients with gingivitis and chronic periodontitis compared to healthy subjects and, determine the correlation between this cytokine and the clinical periodontal parameters, intensity of inflammation and chronic periodontitis severity. Materials and methods: Gingival tissue specimens were taken from 23 chronic periodontitis patients, 20 gingivitis patients and 20 periodontally healthy subjects. The periodontal status was evaluated by dichotomous measurements of the clinical periodontal parameters (PLI, GI, BOP, PPD, CAL). The gingival specimens were fixed immediately in 10% formalin and processed ro
... Show MoreAn interpretive (structural and stratigraphic) study of the two,-dimensional seismic, data of East Nasiriya area (30 km to the south east of Nasiriya oil field within Thi-Qar province, southeastern Iraq) was carried out using Petrel 2017 program. The study area has an importance due to its location between many oil fields, but still without exploration of oil wells. Twenty five seismic lines were used, date back to different types of seismic surveys conducted in the region at different time periods. Also, the seismic velocity surveys of the nearest wells to oil fields, such as Nasiriya-1 and Subba-8, in addition to their sonic and density logs were used. A synthetic seismogram with a good matching with the seismic section was achie
... Show MoreBackground Immunological gene and serum level for interleukin- 9 rs 17317275 have been established to have linked to predisposition systemic lupus erythematosus (SLE) and its severity. SLE is a severe, systemic autoimmune disease characterized by autoantibody generation, complement activation, and immune complex deposition. In the pathophysiology of SLE, cytokines have a pleiotropic function. Recently, IL-9 was discovered to mediate strong anti-inflammatory effects in numerous cells or experimental autoimmune models. Objective This study aimed to determine the role of age, IL-9 serum level and genetic polymorphism, C-reactive protein (CRP), Anti-nuclear antibody (ANA) and Anti- double-stranded DNA (anti-dsDNA) to recognize SLE pathogenesis.
... Show MoreBackground: Thalassemias are a group of heterogeneous genetic disorders, in which the rate of production of hemoglobin is partially or completely suppressed due to reduced rate of synthesis of α or β- chain
Objectives: to estimate the prevalence of Hepatitis C infection among B thalassemia patients attending Ibn-AL-Baladi center of blood diseases in AL-Sader city, in AL-Resafa Quarter of Baghdad and to determine the possible risk factors.
Type of the study: Cross- sectional study.
Methods: A cross sectional study conducted on B Thalassemia patients attending the blood diseases center in Ibn-AL-Baladi hospital during the period from 1st
... Show MoreThe study is an attempt to predict reservoir characterization by improving the estimation of petro-physical properties (porosity), through integration of wells information and 3D seismic data in early cretaceous carbonate reservoir Yamama Formation of (Abu-Amoud) field in southern part of Iraq. Seismic inversion (MBI) was used on post- stack 3 dimensions seismic data to estimate the values of P-acoustic impedance of which the distribution of porosity values was estimated through Yamama Formation in the study area. EMERGE module on the Hampson Russel software was applied to create a relationship between inverted seismic data and well data at well location to construct a perception about the distribution of porosity on the level of all uni
... Show MoreThe characterizations of reservoir require reliable knowledge of certain fundamental reservoir properties. Log measurements can define or at least infer these properties: resistivity, porosity, shale volume, lithology, and water, oil, or gas saturation and permeability. The current study represents evaluation of petrophysical properties in well LU-12 for Zubair Formation in Luhais Oil Field, southern Iraq. The petrophysical evaluation was based on geophysical well logs data to delineate the reservoir characteristics of Zubair Formation. The available geophysical well logs such as (sonic, density, neutron, gamma ray, SP, and resistivity logs) are digitized using the Didger software. The environmental corrections and petrophysical paramete
... Show MoreThirteen isolates were collected from various clinical sources during the periodfrom 22/10/2017 to 22/12/2017. All the isolates were diagnosed based on the microscopic and biochemical propertiesby Vitek-2 Compact system. All isolates formed biofilm 100%, with 30% of isolatesbiofilm produced strongly and 70% on medium. The results of the present study have shown the presence of Curli fimbriae genes in E. cloacae bacteria from cases of urinary tract infections, infected patient with blood bacteremia and inflammation of wounds. Curli fimbriae is considered to be an important factor in the virulence of E.cloacae bacteria, which plays an important role in adhering and combining cells on solid surfaces to form the biofilmand helps in the adhesion
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