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Molecular characterization of HBB gene mutations in beta-thalassemia patients of Southern Iraq
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Introduction and Aim: Beta-thalassemia is a serious inherited genetic disorder and an increasing health burden globally. Beta -thalassemia is caused by genetic globin abnormalities within the hemoglobin beta (HBB) gene. This study aimed to characterize the HBB gene mutations in beta -thalassemia among southern Iraqi patients. Materials and Methods: The study included 30 beta -thalassemia patients referred to the Thi-Qar Center for Genetic Diseases, Iraq and 15 control samples from a random group of apparently healthy individuals. Genomic DNA was isolated from blood sample collected from each individual. The DNA was amplified for specific regions of the HBB gene and the amplified products sequenced. The sequences generated were analysed for mutations using sequence analysis tools. Results: Molecular analysis revealed several mutations in the HBB gene including translocation, deletion and substitution mutations in the population tested positive for the beta -thalassemia trait. Conclusion:  Thalassemia major is a serious concern in southern Iraq and therefore this study emphasizes a need for complete mutation profiling of the beta -globin gene as a strategy for screening of carriers within the population. Such examinations could be useful in pre-marital genetic counseling and for undertaking prevention and treatment measures.

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Publication Date
Fri Jun 30 2023
Journal Name
Iraqi Journal Of Science
Expression of Protamine-1 Gene and Association with Interleukin-12 gene in Infertile Men: A Case-Control Study in Iraqi Patients
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     Approximately 15% of all couples all over the world suffer from difficulty conceiving their first child. The word "infertility" is used to describe this problem.

When a couple had  regular, unprotected sexual intercourse for a year or longer, it indicates that they have been unsuccessful in their efforts to conceive.

      The current investigation aims to find out if there is a relationship between PROTAMIN-1(PRM-1) and INTERLEUKINE-12 (IL-12) gene expressions and their effect on the development of infertility. The current investigation comprised 100 teratozoospermia patients and 100 healthy fertile controls who had their semen examined. Samples were given by Al-Nahrain University's Biotech

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Publication Date
Tue May 30 2023
Journal Name
Iraqi Journal Of Science
Ltb4r Gene Expression in Chronic Myeloid Leukemia in Iraq
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     The current study was carried out to explore gene expression of the LTB4R gene with the development of chronic myeloid leukemia (CML) in Iraqi patients. The differences in the expression of this gene between patients and healthy controls were studied. The correlation of gender and age with CML patients compared with controls was included as well as the correlation of gene expression folding 2-ΔΔCt of LTB4R with clinical parameters (WBC, RBC, haemoglobin, platelets, and BCR-ABL gene). Results revealed significant increases in the mean of gene expression level (ΔCt) of patient groups compared to the corresponding ΔCt means in the healthy control group, the gene expression folding (2-∆∆Ct) of the L

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Publication Date
Tue Mar 30 2021
Journal Name
Iraqi Journal Of Science
A Molecular Study of Toxic Shock Syndrome Toxin gene (tsst-1) in β-lactam Resistant Staphylococcus aureus Clinical Isolates
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Three hundred and sixty different samples were collected from different sources, including wound, burn, nasal, and oral swabs from several hospitals in Baghdad. A number of 150 (53%) Staphylococcus aureus samples were isolated and identified among a total of 283 samples. Then, the spread of the Toxic Shock Syndrome Toxin-1 gene (tsst-1) was investigated in β-lactamase resistant S. aureus. According to the source of samples, the distribution of S. aureus  isolates was found to be significantly higher (p < 0.01) in wound samples as compared to other sources. According to the age, a highly significant  distribution (p < 0.01) was recorded in the age group of 15-30 years,

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Publication Date
Mon Aug 21 2017
Journal Name
Biomedical And Pharmacology Journal
Gene Expression and Clinical Manifestations in Iraqi Patients with Acromegaly
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Publication Date
Thu Feb 01 2024
Journal Name
Baghdad Science Journal
Molecular study of the relationship of gene expression of some genes with the temperature variation of bacterial growth
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Pseudomonas aeruginosa is an opportunistic pathogen responsible for serious infections. At least three different exopolysaccharides, alginate, polysaccharide synthesis locus (Psl), and pellicle exopolysaccharide (Pel) make up the biofilm matrix in P. aeruginosa . The effect of temperature on the biofilm formation and gene expression was examined by microtiter plate and real-time quantitative polymerase chain reaction (qRT-PCR). To be able to determine the effect of temperature on biofilm formation and gene expression of P. aeruginosa, 303 clinical and environmental samples were collected. Pseudomonas aeruginosa was isolated from 61 (20.1%) and 48 (15.8%) of the clinical and e

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Publication Date
Mon Nov 29 2021
Journal Name
Iraqi Journal Of Science
Late Pleistocene - Holocene Paleoecology of Southern Mesopotamia, Iraq
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The distributions of rivers, back swamps, delta, and ancient marine shore line of Southern Mesopotamia during Late Pleistocene – Holocene have been studied depending on the ostracoda and foraminifera assemblages as ecological indicators to determine the ecofacies and paleosalinities, which diagnose different depositional environments. The data are collected from 86 boreholes (including 12 deep boreholes), covering almost the entire Southern Mesopotamia.
Rates of sedimentation are calculated and corrected for compaction in this study, relative to previously measured C14 dating by some authors, for the marine and non- marine (Ur Flood) deposits.
Those rates of sedimentation found in this study are varies from a borehole to another,

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Publication Date
Sun Oct 22 2023
Journal Name
Iraqi Journal Of Science
Morphotectonics of Shatt Al-Arab River Southern Iraq
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A morphotectonic analysis is conducted on Shatt Al-Arab drainage basin. This study aims to analysis of the river patterns of Shatt Al-Arab channel and their relationship with the development of subsurface geological structures and the neotectonic activity, as well as an attempt to determine the relative amount to this activity.
Transverse river profile analysis is derived quantifiable and comparable parameters such as neotectonic index (Eh*Ln), Eh, Ch, and Bs. These parameters are useful to detect the morphotectonic indicators of Shatt Al-Arab basin. The analysis showed the role of the subsurface structures that affecting the river cross sections shape, through channel incision, as in (Dair and NuhrUmr) cross sections, while in the ot

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Publication Date
Wed Feb 01 2017
Journal Name
Heliyon
Environmental assessment of Al-Hammar Marsh, Southern Iraq
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Publication Date
Mon Mar 02 2020
Journal Name
Iraqi Geological Journal
RESERVOIR MODELING OF MISHRIF FORMATION IN MAJNOON OIL FIELD, SOUTHERN IRAQ
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The Middle Cenomanian-Early Turonian Mishrif Formation includes important carbonate reservoirs in Iraq and some other surrounding countries due to their high reservoir quality and wide geological extension. The 2D models of this study for facies, effective porosity and water saturation indicate the vertical and lateral heterogeneity of the Mishrif Formation reservoir properties in the Majnoon oil field. Construction of 2D reservoir model of the Mishrif Formation to explain the distribution of facies and petrophysical properties (effective porosity and water saturation) by using RockWorks software. The increase of effective porosity is attributed to the presence of shoal facies.The high water saturation is attributed to the existence of rest

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Publication Date
Fri Jun 03 2022
Journal Name
International Journal Of Health Sciences
Corrosion risk assessment of the oil flow line in Southern Iraq
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The risk assessment for three pipelines belonging to the Basra Oil Company (X1, X2, X3), to develop an appropriate risk mitigation plan for each pipeline to address all high risks. Corrosion risks were assessed using a 5 * 5 matrix. Now, the risk assessment for X1 showed that the POF for internal corrosion is 5, which means that its risk is high due to salinity and the presence of CO, H2S and POF for external corrosion is 1 less than the corrosion, while for Flowline X2 the probability of internal corrosion is 4 and external is 4 because there is no Cathodic protection applied due to CO2, H2S and Flowline X3 have 8 leaks due to internal corrosion so the hazard rating was very high 5 and could be due to salinity, CO2, fluid flow rate

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