To promote sustainable steel-concrete composite structures, it is essential to develop special shear connectors that facilitate accelerated construction and deconstruction. A lockbolt demountable shear connector (LBDSC) was recently proposed. While the LBDSC has been evaluated using horizontal and vertical (standard) push-out tests, it is essential to further assess the disassembly mechanism and the positive flexural performance of prefabricated demountable composite beams (PDCBs) under both serviceability and ultimate limit states. Two full-scale test specimens of PDCBs with LBDSC were designed with partial shear connections and assessed using a three or four-point load beam setup under both cyclic and static monotonic loading conditions. The experimental results indicate that the failure modes of the PDCBs include longitudinal cracking in the grout between concrete slabs along the composite beam. Additionally, the demountable composite beams exhibited typical ductile flexural failure. The disassembly and reassembly of the PDCB were evaluated using a cyclic loading setup, and the results indicated that the structural performance of the PDCB was not compromised. The experimental results for load-carrying capacity and elastic stiffness of the PDCB were compared to the corresponding values from composite beam theory and the provisions in Eurocode 4. The agreement between the tested and calculated results validates the applicability of the Eurocode 4 equations to demountable composite beams utilizing LBDSCs.