Pseudomonas aeruginosa, a ubiquitous environmental organism, is a difficult-to-treat opportunistic pathogen due to its broad-spectrum antibiotic resistance and its ability to form biofilms. In this study, we investigate the link between resistance to a clinically important antibiotic, imipenem, and biofilm formation. First, we observed that the laboratory strain P. aeruginosa PAO1 carrying a mutation in the oprD gene, which confers resistance to imipenem, showed a modest reduction in biofilm formation.We also observed an inverse relationship between imipenem resistance and biofilm formation for imipenem-resistant strains selected in vitro, as well as for clinical isolates.We identified two clinical isolates of P. aeruginosa from the sputum of cystic fibrosis patients that formed robust biofilms, but were sensitive to imipenem (MIC≤2 μg/ml). To test the hypothesis that there is a general link between imipenem resistance and biofilm formation, we performed transposon mutagenesis of these two clinical strains to identify mutants defective in biofilm formation, and then tested these mutants for imipenem resistance. Analysis of the transposon mutants revealed a role for previously described biofilm factors in these clinical isolates of P. aeruginosa, including mutations in the pilY1, pilX, pilW, algC, and pslI genes, but none of the biofilmdeficient mutants became imipenem resistant (MIC≥8 μg/ml), arguing against a general link between biofilm formation and resistance to imipenem. Thus, assessing biofilm formation capabilities of environmental isolates is unlikely to serve as a good predictor of imipenem resistance. We also discuss our findings in light of the limited literature addressing planktonic antibiotic resistance factors that impact biofilm formation.
Mixed Kirkuk and Sharki-Baghdad crude oils were distilled into narrow fractions. The range of these narrow fractions were 10oC, starting from IBP to 350oC. The total distillates from mixed Kirkuk and Sharki-Baghdad crude oils were 58.25 vol % and 44.65 vol %, respectively.The hydrocarbons compositions (paraffin, naphthene, aromatic) in light fractions starting from IBP to 250oC were determined by using PONA analysis method. The results show that the paraffin content decreases with increasing mid percent boiling point of the fraction, while the naphthene, and aromatic increase with the increase of mid percent boiling point of mixed Kirkuk and Sharki-Baghdad crude oils. Three groups of empirical equations were developed for the prediction
... Show MoreThe aim of the research is to find out the nature of the relationship between the interrelationships and the interrelationships between risk management strategies (avoiding danger, risk exposure, risk exhibition, preventive programs and activities to reduce risks) and the dangers of container transportation, as an independent variable in the dangers of container transportation as a dependent variable, the study started from the problem that the company suffers from The practical aspect of the study was applied at the National Insurance Corporation, and the researcher designed a diagram according to the study's schematic diagram. The study, and for the purpose of data and information related to that, it relied on the descriptive a
... Show MorePurpose: This research seeks to provide a point of view based on the creation of sustainable value to the customer of the banks in the context of total quality management and relationship marketing. It aims to develop a model to measure the value of sustainable customer peduncular under total quality management PAL (administrative leadership, involvement of employees, continuous improvement, process improvement, staff training), through the mediation of relationship marketing and objective dimensions (administrative leadership, involvement of employees, continuous improvement, improving processes , staff training), and to explore any of the variables and dimensions more influential in the creation of sustainable value to the cust
... Show MoreThis paper includes simulating an electron gun design to investigate the effect of the distance between the cathode and the Wehnelt cylinder bore on the quality of the ion beam emittance and to obtain the best distance of that geometrical factor. A study of a group of designs was conducted to find this distance. The SIMION8.0 program has been used to calculate the trajectories of the electrons with initial kinetic energy of (0.1 eV) and electron current of (1×10-12 A). The investigation also includes comparisons between the equipotential line trajectories for each design as well as the field distribution and the electron trajectories. The best emittance distance was concluded to be (3 mm).
The Fort Munro Formation is well exposed along DG Khan-Fort Munro road, a type locality. The section was measured and sampled for the biostratigraphy, where it has a conformable lower contact with Mughalkot Formation and is underlain by the Pab Sandstone. The total observed thickness was 105 meters, and 26 samples were collected from top to the bottom at random intervals. Fifty thin sections were studied carefully, and five species of large benthic foraminifera, including Orbitoidestissoti, Orbitoidesapiculata, Orbitoides media, Orbitoideshottingeri, and Omphalocyclusmacroporus were identified along with miliolids. Gastropods, Bivalves and Echinoderms were also observed. Based on identified microfossils, the de
... Show MoreThis study deals with the petrology of the lower succession of the Injana Formation in the Shorr Shareen area, Wasit Governorate, Eastern Iraq. The study revealed that the sandstone is litharenite consists of 45.56% rock fragments, 22.13% quartz and 8.5% feldspars. The matrix is about 8.39%, consisting of silt and clay particles. The cement is variable (carbonates 8.42%, evaporites 1.78% and iron oxides 0.96%). The grain assemblage infers that the source of the rock fragments is nearby. The petrographic analyses indicate that the studied Injana sandstones are immature mineralogically because of their content of unstable constituents, such as lithic fragments and feldspars. In addition, the presence of such fresh feldspars indica
... Show MoreSedimentary structures of Gercus Formation in NE Iraq was little studied in the last decades. In this study the identified sedimentary structures display alternative graded and fining upward cycles, load and flute casts, submarine channels, sand and clay balls and pillow structures, convolute and slump beddings, of marine turbidity origin. The foreland part of Tethys basin characterized by deep marine Tanjero and Kolosh Formations followed by the Gercus formation with conformable relationships. The Eocene aged Flysch comprises predominantly litharenitic sandstones and interbedded mudstones, both of turbiditic affinities and most likely derived from a NE Arabian Plate source. The sediments provide excellent examples of distal fan sands as
... Show MoreThis paper contains studying of the Evaluation for the Petrophysical Properties of
Yamama Formation in Ratawi Field which occurs in about 70 km to the west of
Basrah city in Mesopotamia zone (Zubair subzone). The study includes a
petrophysical evaluation and (3 Dimensions) geological model for each unit
especially the three hydrocarbon units comprising the Yamama Formation in (5)
boreholes which are Rt-3, Rt-4, Rt-5, Rt-6 and Rt-7 distributed on the crest and
flanks of the Ratawi structure that are carried out in the present study. The
formation's boundaries were determined using well logs, available core intervals and
by Petrophysical data and it is found that it can be subdivided into three main
reservoir uni
The major objective of this paper is to recognize the flow units of Yamama Formation in the west Qurna oil field, south of Iraq. To attain this objective, four wells namely, WQ-23, WQ-148, WQ-60, and WQ-203 are selected and analyzed. The two techniques hat proposed by some scientists to identify flow units are tested and verified. Results are also enhanced using well logs interpretation and the flow areas are proposed through the studying of the behavior of different well logs. Results of applying the two proposed techniques identify six flow reservoir units for the wells WQ-23, WQ-148, WQ-60, and WQ-203, respectively. This study also shows that the flow reservoir properties in the Yamama Formation improved towards the northeast of the W
... Show More