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Gentamicin Modulates the Gene Expression of hla in Methicillin Resistance Staphylococcus aureus Biofilm
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Objective: The present work was undertaken to investigate the impact of sub inhibitory concentration of gentamicin on hla gene expression in methicillin resistant Staphylococcus aureus isolates. Methods: The bacterial isolates used in this study represent 33 MRSA strains, previously isolated form patients visiting several hospitals in Baghdad. Gentamicin, vancomycin, and oxacillin MIC were determined using broth dilution method. Microtiter plate method was adopted to investigate the biofilm forming capacity. Alpha hemolysin was detected by culturing MRSA isolates on rabbit blood agar. Furthermore, hla gene was detected in MRSA isolates using conventional PCR technique; while, qRT-PCR method was performed to assay the hla expression in planktonic and biofilm cells in presence and absence of gentamicin. Results: the present results demonstrated that 12 (36.36%) isolates were gentamicin-resistant; whereas, all isolates were resistant to oxacillin and sensitive to vancomycin. Out of 33 MRSA, 3, 23, and 7 isolates formed a weak, moderate, and strong biofilm, respectively. Phenotypically, 30 isolates produced alpha hemolysin on rabbit blood agar plates; nevertheless, hla gene was located in 29 isolates. Of considerable interest, the addition of gentamicin significantly (P < 0.05) reduced the hemolysis activity; while, insignificant fold change (less than two) of hla gene was observed in all tested isolates in the presence of sub MIC of gentamicin (16 µg/ml). Conclusion: gentamicin upregulated the hla gene expression in biofilm cells; hitherto, this increment was isolate specific.

Publication Date
Fri Nov 01 2024
Journal Name
Cytokine
Lupeol stimulates iNOS, TNF-α, and IL-10 expression in the U937 cell line infected with old-world Leishmania donovani
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Publication Date
Tue Mar 01 2016
Journal Name
Al-academy
THE METHOD OF EXPRESSION IN INNATE AND POPULAR IRAQI SCULPTURAL CONTEMPORARY STUDYING BETWEEN (Hadi KadhimNassif) and (Ibrahim al nakash): كفاح عبد المجيد
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The innate art,is a major tributary in the art world, It is a bold achievement, has a special flavor, With self-identity, linked to the origins and roots of human ,That goes back to the past and the history and culture of peoples. This business is not limited to only aesthetic side, but is loaded with religious and magical experience, and have social functions, This creations stayed live, breathe, grow, and change with the transformation of society, these processors emerged, from local people have not been trained academically.Their Elvis came from community culture,Which are, Mesopotamian thoroughbreds.This culture that we desperately need today, in order to increase ourselves Iraqi force, In the face of the modern world, which is locke

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Publication Date
Thu Apr 25 2019
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
STUDY OF THE SYNERGISTIC EFFECT OF BACTERIOCIN PRODUCED FROM Leuconostoc mesenteroides ssp. cremoris With Antibiotics Against Multi Resistance Diarrheal Bacteria: STUDY OF THE SYNERGISTIC EFFECT OF BACTERIOCIN PRODUCED FROM Leuconostoc mesenteroides ssp. cremoris With Antibiotics Against Multi Resistance Diarrheal Bacteria
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The study included the collection of samples of raw cow milk to isolate Leuconostoc bacteria, samples were sub cultured on De-Man Rogosa Sharpe-Vancomycin medium, the pure colonies were selected and subjected to the cultural and microscopically tests, according to that 25 cocci bacterial isolates were obtained, then isolates were subjected to biochemical tests. Result of tests showed that 12 isolates belong to the genus Leuconostoc out of 25 cocci bacterial isolates, Vitek2 system was used as a supplementary step. Results of final identification showed that 3 sub species were obtained included Leuconostoc mesenteroides ssp. cremoris 9 out of 12 isolates, while it was 2 isolates of Leuconostoc mesenteroides ssp. mesenteroides and one isol

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Publication Date
Mon Jun 12 2006
Journal Name
Iraqi Journal Of Laser
Laser-Induced Low-Resistance Ohmic Contacts on n-Si
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In the present work, the feasibility of formation near-ideal ohmic behavior of In/n-Si contact efficiently by 300 s duration Nd:YAG pulsed laser processing has been recognized. Several laser pulses energy densities have been used, and the optimal energy density that gives best results is obtained. Topography of the irradiated region was extensively discussed and supported with micrographic illustrations to determine the surface condition that can play the important role in the ohmic contact quality. I-V characteristics in the forward and reverse bias and barrier height measurements have been studied for different irradiated samples to determine the laser energy density that gives best ohmic behavior. Comparing the current results with

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Publication Date
Wed Jan 01 2020
Journal Name
University Of Bucharest Rom Biotechnol Printed In Romania.
Antibiotic resistance determinants of Acinetobacter baumannii strains isolated from nosocomial infections
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Publication Date
Wed Jun 24 2026
Journal Name
Journal Of Applied Animal Research
Antimicrobial resistance of <i>Escherichia coli</i> isolated from broiler chicken
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Recent studies highlighted the hazard of emerging antimicrobial resistance (AMR) bacteria in domesticated animals, with multidrug resistance (MRD) patterns in poultry recorded with alarming intensity as a major reservoir. This resistance has been associated with the misuse and overuse of antimicrobial agents in poultry farms. This current study aimed to determine the multidrug resistance (MDR) patterns of Escherichia coli isolated from broiler chickens. A total of 32 human samples were taken from worker handlers (positive: 4 samples, 12.5%), and 40 samples were collected from poultry chickens (positive: 13 samples, 32.5%). The findings revealed marked resistance against six of the ten agents in the isolates (MDR being 100% in all samples an

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Publication Date
Mon Mar 08 2021
Journal Name
Baghdad Science Journal
Effect of growth regulators on sex expression and some quantitatve properties
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This study was conducted in an orchard pomegranate's Department of Horticulture College of Agriculture, University of Baghdad for two seasons 1999-2000 on cultivars pomegranate Salimi and narrators seedless to study the effect spraying Nizant growth in sex ratio of flowers and recipes flowering and winning was selected 27 trees per class 15 years old planted

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Publication Date
Sat Jul 22 2023
Journal Name
Journal Of Engineering
Investigation Parameters of Resistance Spot Welding For AA1050 Aluminum Alloy Sheets
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The parameters of resistance spot welding (RSW) performed on low strength commercial aluminum sheets are investigated experimentally, the performance requirements and weldability issues were driven the choice of a specific aluminum alloy that was AA1050. RSW aluminum alloys has a major problem of inconsistent quality from weld to weld comparing with welding steel
alloys sheet, due to the higher thermal conductivity, higher thermal expansion, narrow plastic temperature range, and lower electrical resistivity. Much effort has been devoted to the study of describing the relation between the parameters of the process (welding current, welding time, and electrode force) and weld strength. Shear-tensile strength tests were performed to ind

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Publication Date
Thu Jul 20 2023
Journal Name
Polymers In Medicine
Effect of subinhibitory doses of rifaximin on in vitro Pseudomonas aeruginosa adherence and biofilm formation to biotic and abiotic surface models
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Publication Date
Sun Sep 01 2024
Journal Name
Al-rafidain Journal Of Medical Sciences ( Issn 2789-3219 )
The Sensitivity of Immunohistochemical Expression of p53 as an Indicator of the Malignant Potential of Gastric Hyperplastic Polyps: A Retrospective Study
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Background: Hyperplastic polyps account for 30–93% of gastric polyps. Recently, there have been studies about the development of dysplasia in this type of polyp. Every cell in the body contains the p53 gene, which has anti-cancer properties. Objective: The sensitivity of immunohistochemical expression of p53 is evaluated among gastric hyperplastic polyps with and without dysplasia and gastric adenomatous polyps to know its usefulness as a diagnostic marker. Methods: A retrospective cross-sectional study was done on fifty formalin-fixed paraffin-embedded blocks of gastric polyps (44 hyperplastic polyps without dysplasia, 3 hyperplastic polyps with dysplasia, and 3 adenomas). Cases were collected from the archives of the pathology d

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