Nigella sativa has various pharmacological properties and has been used throughout history for a variety of reasons. However, there is limited data about the effects of N. sativa (NS) on human cancer cells. This study aimed at observing the roles of methanolic extract of N. sativa on apoptosis and autophagy pathway in the Human PC3 (prostate cancer) cell line. The cell viability was checked by MTT assay. Clonogenic assay was performed to demonstrate clonogenicity and Western blot was used to check caspase-3, TIGAR, p53, and LC3 protein expression. The results demonstrated that PC3 cell proliferation was inhibited, caspase-3 and p53 protein expression was induced, and LC3 protein expression was modulated. The clonogenic assay showed that PC3 cell line colonization was restricted. To conclude: N. sativa methanol extract had potent anti-cancer effects that inhibited cell viability, induced apoptosis, and inhibited clonogenicity in the PC3 cell line.
A series of new copolyimides containing pendant 1,3,4-oxadiazole moiety were synthesized via multisteps. In the first step five N-(5-substituted-1,3,4-oxadiazole-2-yl)maleamic acids were prepared via reaction of maleic anhydride with 2-amino-5-substituted-1,3,4-oxadiazoles. The obtained amic acids were dehydrated in the second step affording the corresponding N-(5-substituted-1,3,4-oxadiazole-2-yl) maleimides. In the third step the newly synthesized maleimides were introduced successfully in free radical copolymerization reaction with four vinylic monomers including acrylo nitrile, methacrylonitrile, methyl acrylate and methyl meth acrylate respectively producing twelve new copolymers having different physical properties which may serve
... Show MoreThe current study aimed to synthesize silver nanoparticles (Ag NPs) in a safe and eco-friendly biological method using green tea extract, characterize them using UV-vis spectroscopy, FTIR, XRD, FESEM, and AFM tools, then investigate their antioxidant potentials against DPPH and their cytotoxicity toward lymphoma cells using MTT and ethidium bromide/propidium iodide dual staining assays, and their genotoxic capacities by assessing their effects on the gene expression of p53, caspase3, bcl-2, and bax genes by qPCR and DNA fragmentation. Results confirmed the synthesis of silver nanoparticles; the UV-vis results showed a peak of absorbance at 414 nm; FTIR analysis showed absorbance bel
... Show MoreThe current study was designed to evaluate the anti-inflammatory effect of GKB in the rat model of granulomatous inflammation. Thirty rats were distributed into five groups: The first group served as negative control group that received distilled water (DW) only without inducting inflammation, positive control group; treated with DW with the induction of inflammation and they were assigned to cotton pellet-induced granuloma, ginkgo biloba (GKB) treated group (200mg/kg/day), dexamethasone-treated group (1mg/kg), and Prednisolone treated group (5mg/kg). All the treatments were given orally for seven consecutive days. On day eight, the rats were anesthetized and the pellets together with granulation tissue were carefully removed
... Show MoreIn Iraq; there is a great demand for handling some epidemic diseases such as hyperglycemia and hyperlipidema through searching some medical plant extracts which the country is rich with and contains an important phytochemicals that may solve the incidence of such cases. Aloe vera that cultivated in Iraq, is known to be rich with biological active constituents. The aim of the present study; the plant gel extract was subjected to treat an induced diabetic lab mice through injecting streptozotocin dose with high fatty food to elevate lipid profile before induction of the gel oral treatment of the plant. In the study results: the oral administration of A.ver
... Show MoreIn this work, watershed transform method was implemented to detect and extract tumors and abnormalities in MRI brain skull stripped images. An adaptive technique has been proposed to improve the performance of this method.Watershed transform algorithm based on clustering techniques: K-Means and FCM were implemented to reduce the oversegmentation problem. The K-Means and FCM clustered images were utilized as input images to the watershed algorithm as well as of the original image. The relative surface area of the extracted tumor region was calculated for each application. The results showed that watershed trnsform algorithm succeedeed to detect and extract the brain tumor regions very well according to the consult of a specialist doctor a
... Show MoreA new, simple, accurate, fast and sensitive spectrophotometric method has been developed for the analysis of amiloride in pure and pharmaceutical formulations by continuous flow injection analysis. The method was based on the oxidation of the drug with Ce(IV)sulfate in acidic medium to formed a pinkish-red color species which determined using homemade Ayah 3SBGR x3-3D solar cell flow injection photometer. Optimum conditions were obtained using a high intensity green light emitted diode as a source. The linear dynamic range for the instrument response versus amiloride concentration was 0.0005- 10 mmol.L-1 while the L.O.D was of 9.471 ng / sample. The correlation coefficient (r) was 0.9824 while percentage linearity (r2%) was 96.50%. RSD%
... Show MoreNew chelating ligand derived from triazole and its complexes with metal ions Rhodium, Platinum and Gold were synthesized. Through a copper (I)-catalyzed click reaction, the ligand produced 1,3-dipolar cycloaddition between 2,6-bis((prop-2-yn-1-yloxy) methyl) pyridine and 1-azidododecane. All structures of these new compounds were rigorously characterized in the solid state using spectroscopic techniques like: 1HNMR, 13CNMR, Uv-Vis, FTIR, metal and elemental analyses, magnetic susceptibility and conductivity measurements at room temperature, it was found that the ligand acts as a penta and tetradentate chelate through N3O2, N2O2, and the geometry of the new complex
... Show MoreNew chelating ligand derived from triazole and its complexes with metal ions Rhodium, Platinum and Gold were synthesized. Through a copper (I)-catalyzed click reaction, the ligand produced 1,3-dipolar cycloaddition between 2,6-bis((prop-2-yn-1-yloxy) methyl) pyridine and 1-azidododecane. All structures of these new compounds were rigorously characterized in the solid state using spectroscopic techniques like: 1HNMR, 13CNMR, Uv-Vis, FTIR, metal and elemental analyses, magnetic susceptibility and conductivity measurements at room temperature, it was found that the ligand acts as a penta and tetradentate chelate through N3O2, N2O2, and the geometry of the new complexes are identified as octahedral for (Rh & Pt) complexes a
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